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Blood, 1 December 2000, Vol. 96, No. 12, pp. 3979-3981
BRIEF REPORT
High marrow seeding efficiency of human lymphomyeloid
repopulating cells in irradiated NOD/SCID
mice
Johanne D. Cashman and
Connie J. Eaves
From the Terry Fox Laboratory, British Columbia Cancer
Agency, and the Department of Medical Genetics, University of British
Columbia Vancouver, BC, Canada.
Transplantable human hematopoietic stem cells (competitive
repopulating units [CRU]) can be quantitated based on their ability to produce large populations of lymphoid and myeloid progeny within 6 weeks in the marrow of intravenously injected, sublethally irradiated NOD/SCID mice. It is shown that the proportions of total
injected human fetal liver and cord blood CRU in the marrow of mice 24 hours after transplantation are 5% and 7%, respectively, as
determined by limiting-dilution assays in other primary and secondary
NOD/SCID mice. The similarity in these 2 seeding efficiency values
suggests that mechanisms regulating the ability of human hematopoietic stem cells to enter the marrow from the blood, at least in this xenotransplant model, do not change between fetal life and
birth. In addition, it appears that previously reported human stem cell frequencies and their in vivo self-renewal activity measured in NOD/SCID mice have been markedly underestimated.
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