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Blood, 1 December 2000, Vol. 96, No. 12, pp. 3985-3987
BRIEF REPORT
An expansion phase precedes terminal erythroid
differentiation of hematopoietic progenitor cells from cord blood in
vitro and is associated with up-regulation of cyclin E and
cyclin-dependent kinase 2
Mu-Shui Dai,
Charlie R. Mantel,
Zhen-Biao Xia,
Hal E. Broxmeyer, and
Li Lu
From the Departments of Microbiology/Immunology,
Medicine (Hematology/ Oncology), and The Walther
Oncology Center, Indiana University School of Medicine, Indianapolis,
and the Walther Cancer Institute, Indianapolis, IN.
The dynamics of cell cycle regulation were investigated during in
vitro erythroid proliferation and differentiation of CD34+
cord blood cells. An unusual cell cycle profile with a majority of
cells in S phase (70.2%) and minority of cells in G1 phase (27.4%)
was observed in burst-forming unit-erythrocytes (BFU-E)-derived erythroblasts from a 7-day culture of CD34+ cells
stimulated with interleukin 3 (IL-3), granulocyte-macrophage colony-stimulating factor (GM-CSF), Steel factor, and Epo. Terminal erythroid differentiation was accompanied by a rapid increase of G0/G1
phase cells. Expression of cyclin E and cyclin-dependent kinase 2 (cdk2) correlated with the proportion of S phase cells. Cyclin D3 was
moderately up-regulated during the proliferation phase, and both cyclin
E and D3 were rapidly down-regulated during terminal
differentiation. This suggests that the high proliferation potential of
erythroblasts is associated with temporal up-regulation of cyclin E and cdk2.
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