|
|||||||||
|
|
|
|
|
|
|
|
|
|
|
IMMUNOBIOLOGY
From the Department of Immunology, Saga Medical School,
Nabeshima, Japan.
Conflicting findings regarding proadhesion and antiadhesion
in cell-to-cell interactions were previously reported for CD43. We
examined possible differences in the role of the 130-kd glycoform and
the 115-kd glycoform of CD43 in cellular adhesion in vitro. We
generated a monoclonal antibody (MFT3) that discriminates between helper and nonhelper murine T-cell clones. Characterization of MFT3 with use of biochemical analysis and complementary DNA (cDNA) transfection experiments showed that it is specific for the 130-kd glycoform of CD43. T-cell clones that expressed the 130-kd CD43 glycoform showed decreased homocytic aggregation and decreased adhesion
to spleen cells, B-lymphoma cell lines, and fibroblastic cell lines
compared with T-cell clones negative for the 130-kd glycoform.
Expression of core 2 This article has been cited by other articles:
| |||||||||||
 |
 |
 |
|||||||
 |
 |
 |
 |
 |
 |
 |
 |
||
| Copyright © 2000 by American Society of Hematology Online ISSN: 1528-0020 | |||||||||
-1,
6-N-acetylglucosaminyltransferase (C2GnT) cDNA together
with CD43 cDNA resulted in expression of both the 130-kd CD43 glycoform
and the 115-kd CD43 glycoform in fibroblastic cell lines. Using these
cell lines, we showed that the 130-kd glycoform but not the 115-kd
glycoform of CD43 has an antiadhesive function in cellular
interactions. Our findings suggest that the antiadhesive function of
CD43 is primarily carried out by the 130-kd glycoform.
