|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 398-404
PLENARY PAPER
Detection of differentially expressed genes in lymphomas using
cDNA arrays: identification of clusterin as a new diagnostic marker for
anaplastic large-cell lymphomas
Axel Wellmann,
Catherine Thieblemont,
Stefania Pittaluga,
Akira Sakai,
Elaine S. Jaffe,
Paul Siebert, and
Mark Raffeld
From the Hematopathology Section, Laboratory of Pathology, National
Cancer Institute, National Institutes of Health, Bethesda, MD; and Gene
Cloning & Analysis, Clontech Laboratories, Palo Alto, CA.
This study reports the first use of gene array technology for the
identification of a tumor-specific marker in lymphoid neoplasms. The
differential gene expression of 31 hematopoietic cell lines, representing most major lymphoma subgroups of B- and T-cell origin, was
assessed by hybridizing labeled complementary DNA to Atlas human
expression arrays containing 588 genes. Genes known to be specific for
B, T, or myelomonocytic lineages were appropriately identified in the
arrays, validating the general utility of this approach. One gene,
clusterin, not previously known to be expressed in lymphoid
neoplasms, was specifically found in all 4 anaplastic large-cell
lymphoma (ALCL) cell lines, but not in any of the 27 remaining tumor
lines. Using a monoclonal antibody against clusterin, its differential
expression was confirmed by Western blotting and immunohistochemistry.
A total of 198 primary lymphomas (representing most major lymphoma
subtypes), including 36 cases of systemic ALCL, were surveyed for
clusterin expression by immunohistochemistry and Western blotting. All
of the 36 ALCL cases marked for clusterin, with most cases showing
moderate to strong staining in the majority of neoplastic cells.
Clusterin expression was not related to expression of anaplastic
lymphoma kinase-1. With 2 exceptions, none of the remaining 162 non-ALCL cases marked with the clusterin antibody, including Hodgkin
disease and primary cutaneous ALCL. In reactive lymphoid tissues, only
follicular dendritic cells and fibroblastic reticular cells exhibited
staining. Clusterin is a highly conserved glycoprotein implicated in
intercellular and cell matrix interactions, regulation of the
complement system, lipid transport, stress responses, and apoptosis.
Although its function in ALCL is unknown, the unique expression of
clusterin within this category of lymphoma provides an additional
marker for the diagnosis of ALCL. This study illustrates the enormous
potential of gene array technologies for diagnostic marker discovery.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
U. Bacher, A. Kohlmann, and T. Haferlach
Perspectives of gene expression profiling for diagnosis and therapy in haematological malignancies
Brief Funct Genomic Proteomic,
May 27, 2009;
(2009)
elp011v1.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Chandra, J. A. Plaza, Z. Zuo, A. H. Diwan, H. Koeppen, M. Duvic, L. J. Medeiros, and V. G. Prieto
Clusterin Expression Correlates With Stage and Presence of Large Cells in Mycosis Fungoides
Am J Clin Pathol,
April 1, 2009;
131(4):
511 - 515.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Lamant, A. d. Reynies, M.-M. Duplantier, D. S. Rickman, F. Sabourdy, S. Giuriato, L. Brugieres, P. Gaulard, E. Espinos, and G. Delsol
Gene-expression profiling of systemic anaplastic large-cell lymphoma reveals differences based on ALK status and two distinct morphologic ALK+ subtypes
Blood,
March 1, 2007;
109(5):
2156 - 2164.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Quintanilla-Martinez, S. Pittaluga, C. Miething, M. Klier, M. Rudelius, T. Davies-Hill, N. Anastasov, A. Martinez, A. Vivero, J. Duyster, et al.
NPM-ALK-dependent expression of the transcription factor CCAAT/enhancer binding protein beta in ALK-positive anaplastic large cell lymphoma
Blood,
September 15, 2006;
108(6):
2029 - 2036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Rodriguez-Pineiro, M. P. de la Cadena, A. Lopez-Saco, and F. J. Rodriguez-Berrocal
Differential Expression of Serum Clusterin Isoforms in Colorectal Cancer
Mol. Cell. Proteomics,
September 1, 2006;
5(9):
1647 - 1657.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Jacobsen
Anaplastic Large-Cell Lymphoma, T-/Null-Cell Type
Oncologist,
July 1, 2006;
11(7):
831 - 840.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. So, S. Sinnemann, D. Huntsman, L. Fazli, and M. Gleave
Knockdown of the cytoprotective chaperone, clusterin, chemosensitizes human breast cancer cells both in vitro and in vivo
Mol. Cancer Ther.,
December 1, 2005;
4(12):
1837 - 1849.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R Rust, G Harms, T Blokzijl, M Boot, A Diepstra, J Kluiver, L Visser, S-C Peh, M Lim, W A Kamps, et al.
High expression of Mcl-1 in ALK positive and negative anaplastic large cell lymphoma
J. Clin. Pathol.,
May 1, 2005;
58(5):
520 - 524.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Janik, J. C. Morris, S. Pittaluga, K. McDonald, M. Raffeld, E. S. Jaffe, N. Grant, M. Gutierrez, T. A. Waldmann, and W. H. Wilson
Elevated serum-soluble interleukin-2 receptor levels in patients with anaplastic large cell lymphoma
Blood,
November 15, 2004;
104(10):
3355 - 3357.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. L. Ebert and T. R. Golub
Genomic approaches to hematologic malignancies
Blood,
August 15, 2004;
104(4):
923 - 932.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. P. Trougakos, A. So, B. Jansen, M. E. Gleave, and E. S. Gonos
Silencing Expression of the Clusterin/Apolipoprotein J Gene in Human Cancer Cells Using Small Interfering RNA Induces Spontaneous Apoptosis, Reduced Growth Ability, and Cell Sensitization to Genotoxic and Oxidative Stress
Cancer Res.,
March 1, 2004;
64(5):
1834 - 1842.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. TURECI, H. BIAN, F. O. NESTLE, L. RADDRIZZANI, J. A. ROSINSKI, A. TASSIS, H. HILTON, M. WALSTEAD, U. SAHIN, and J. HAMMER
Cascades of transcriptional induction during dendritic cell maturation revealed by genome-wide expression analysis
FASEB J,
May 1, 2003;
17(8):
836 - 847.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Nishikori, Y. Maesako, C. Ueda, M. Kurata, T. Uchiyama, and H. Ohno
High-level expression of BCL3 differentiates t(2;5)(p23;q35)-positive anaplastic large cell lymphoma from Hodgkin disease
Blood,
April 1, 2003;
101(7):
2789 - 2796.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. TURECI, J. DING, H. HILTON, H. BIAN, H. OHKAWA, M. BRAXENTHALER, G. SEITZ, L. RADDRIZZANI, H. FRIESS, M. BUCHLER, et al.
Computational dissection of tissue contamination for identification of colon cancer-specific expression profiles
FASEB J,
March 1, 2003;
17(3):
376 - 385.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. D. Medh
Microarray-Based Expression Profiling of Normal and Malignant Immune Cells
Endocr. Rev.,
June 1, 2002;
23(3):
393 - 400.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Husson, E. G. Carideo, D. Neuberg, J. Schultze, O. Munoz, P. W. Marks, J. W. Donovan, A. C. Chillemi, P. O'Connell, and A. S. Freedman
Gene expression profiling of follicular lymphoma and normal germinal center B cells using cDNA arrays
Blood,
January 1, 2002;
99(1):
282 - 289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. M. Weitzel, C. Radtke, and H. J. Seitz
Two thyroid hormone-mediated gene expression patterns in vivo identified by cDNA expression arrays in rat
Nucleic Acids Res.,
December 15, 2001;
29(24):
5148 - 5155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Z. Rassidakis, A. H. Sarris, M. Herling, R. J. Ford, F. Cabanillas, T. J. McDonnell, and L. J. Medeiros
Differential Expression of BCL-2 Family Proteins in ALK-Positive and ALK-Negative Anaplastic Large Cell Lymphoma of T/Null-Cell Lineage
Am. J. Pathol.,
August 1, 2001;
159(2):
527 - 535.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
W.-K. Hofmann, S. de Vos, K. Tsukasaki, W. Wachsman, G. S. Pinkus, J. W. Said, and H. P. Koeffler
Altered apoptosis pathways in mantle cell lymphoma detected by oligonucleotide microarray
Blood,
August 1, 2001;
98(3):
787 - 794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. P. Greer, M. C. Kinney, and T. P. Loughran Jr.
T Cell and NK Cell Lymphoproliferative Disorders
Hematology,
January 1, 2001;
2001(1):
259 - 281.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
