|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 601-609
New induction of leukotriene
A4 hydrolase by interleukin-4 and interleukin-13 in human
polymorphonuclear leukocytes
Masafumi Zaitsu,
Yuhei Hamasaki,
Muneaki Matsuo,
Akiko Kukita,
Kosuke Tsuji,
Michiko Miyazaki,
Rika Hayasaki,
Eriko Muro,
Shuichi Yamamoto,
Ikuko Kobayashi,
Tomohiro Ichimaru,
Osamu Kohashi, and
Sumio Miyazaki
From the Departments of Pediatrics and Microbiology, Saga Medical
School, Saga, Japan.
Interleukin (IL)-4, IL-10, and IL-13, Th2 cell-derived cytokines,
play major roles in the pathophysiology of allergic diseases. These
cytokines up-regulate or down-regulate the production of arachidonic
acid metabolites. In this study, we have investigated the effect of
IL-4, IL-10, IL-13, and other cytokines on A23187-stimulated synthesis
of leukotriene (LT) B4 in human polymorphonuclear
leukocytes (PMNs). Production of LTB4 was measured by
specific radioimmunoassay and high performance liquid chromatography.
Messenger RNA (mRNA) expression of cytosolic phospholipase
A2 (cPLA2), 5-lipoxygenase (5-LO), and
LTA4 hydrolase, which were involved in the synthesis of
LTB4, was determined by reverse transcription-polymerase
chain reaction and Northern blot analysis. Protein synthesis of
their enzymes was determined by Western blot analysis.
IL-4 and IL-13 enhanced A23187-stimulated LTB4 synthesis
and increased mRNA expression and protein synthesis of LTA4
hydrolase, but not those of cPLA2 or 5-LO.
These results indicate that IL-4 and IL-13 transcriptionally or
post-transcriptionally up-regulate the synthesis of LTB4, a potent chemotactic factor to PMNs, at the enzyme level of
LTA4 hydrolase, and this up-regulation mechanism may
participate in the development of allergic inflammation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
B. D. Levy, P. Kohli, K. Gotlinger, O. Haworth, S. Hong, S. Kazani, E. Israel, K. J. Haley, and C. N. Serhan
Protectin D1 Is Generated in Asthma and Dampens Airway Inflammation and Hyperresponsiveness
J. Immunol.,
January 1, 2007;
178(1):
496 - 502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T Gebhardt, A Lorentz, F Detmer, C Trautwein, H Bektas, M P Manns, and S C Bischoff
Growth, phenotype, and function of human intestinal mast cells are tightly regulated by transforming growth factor {beta}1
Gut,
July 1, 2005;
54(7):
928 - 934.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Kolodsick, G. B. Toews, C. Jakubzick, C. Hogaboam, T. A. Moore, A. McKenzie, C. A. Wilke, C. J. Chrisman, and B. B. Moore
Protection from Fluorescein Isothiocyanate-Induced Fibrosis in IL-13-Deficient, but Not IL-4-Deficient, Mice Results from Impaired Collagen Synthesis by Fibroblasts
J. Immunol.,
April 1, 2004;
172(7):
4068 - 4076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Chen, N. Li, S. Wang, N. Wu, J. Hong, X. Jiao, M. J. Krasna, D. G. Beer, and C. S. Yang
Leukotriene A4 Hydrolase in Rat and Human Esophageal Adenocarcinomas and Inhibitory Effects of Bestatin
J Natl Cancer Inst,
July 16, 2003;
95(14):
1053 - 1061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zaitsu, Y. Hamasaki, K. Tsuji, M. Matsuo, I. Fujita, Y. Aoki, E. Ishii, and O. Kohashi
Dexamethasone accelerates catabolism of leukotriene C4 in bronchial epithelial cells
Eur. Respir. J.,
July 1, 2003;
22(1):
35 - 42.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Harizi, M. Juzan, J.-F. Moreau, and N. Gualde
Prostaglandins Inhibit 5-Lipoxygenase-Activating Protein Expression and Leukotriene B4 Production from Dendritic Cells Via an IL-10-Dependent Mechanism
J. Immunol.,
January 1, 2003;
170(1):
139 - 146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Smith, C. A. Evans, A. Pierce, S. J. Gaskell, and A. D. Whetton
Changes in the Proteome Associated with the Action of Bcr-Abl Tyrosine Kinase Are Not Related to Transcriptional Regulation
Mol. Cell. Proteomics,
November 1, 2002;
1(11):
876 - 884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Crepaldi, L. Silveri, F. Calzetti, C. Pinardi, and M. A. Cassatella
Molecular basis of the synergistic production of IL-1 receptor antagonist by human neutrophils stimulated with IL-4 and IL-10
Int. Immunol.,
October 1, 2002;
14(10):
1145 - 1153.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Profita, A. Sala, L. Siena, P. M. Henson, R. C. Murphy, A. Paterno, A. Bonanno, L. Riccobono, A. Mirabella, G. Bonsignore, et al.
Leukotriene B4 Production in Human Mononuclear Phagocytes Is Modulated by Interleukin-4-Induced 15-Lipoxygenase
J. Pharmacol. Exp. Ther.,
March 1, 2002;
300(3):
868 - 875.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Eberhard, S. Jepsen, L. Pohl, H. K. Albers, and Y. Acil
Bacterial Challenge Stimulates Formation of Arachidonic Acid Metabolites by Human Keratinocytes and Neutrophils In Vitro
Clin. Vaccine Immunol.,
January 1, 2002;
9(1):
132 - 137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Thivierge, J. Stankova, and M. Rola-Pleszczynski
IL-13 and IL-4 Up-Regulate Cysteinyl Leukotriene 1 Receptor Expression in Human Monocytes and Macrophages
J. Immunol.,
September 1, 2001;
167(5):
2855 - 2860.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
