|
|
 Previous Article | Table of Contents | Next Article 
Blood, Vol. 96 No. 2 (July 15), 2000:
pp. 771-772
BRIEF REPORT
Immunohistochemical staining for megakaryocyte c-mpl may
complement morphologic distinction between polycythemia vera and
secondary erythrocytosis
Ayalew Tefferi,
Soo-Young Yoon, and
Chin-Yang Li
From the Division of Hematology and Internal Medicine and the
Division of Hematopathology, Mayo Clinic and Mayo Foundation,
Rochester, MN.
Recent studies have shown decreased megakaryocyte expression of the
thrombopoietin receptor (c-mpl) in patients with polycythemia vera (PV) but not in those with reactive erythrocytosis. We examined the diagnostic utility of this observation in 22 patients with PV, 7 patients with secondary erythrocytosis (SE), and 10 normal controls.
Commercial antibodies against c-mpl were used with standard immunoperoxidase methods. Megakaryocyte c-mpl staining
intensity was uniformly moderate-to-strong in the healthy controls and
in all the patients with SE. In contrast, staining intensity in 9 patients with PV (41%) was uniformly weak. Furthermore, in 12 of the
remaining 13 patients with PV, the c-mpl staining pattern in
each case was heterogeneous and was associated with weak staining intensity in more than 20% of the megakaryocyte population. These preliminary data suggest that c-mpl immunostains may complement bone marrow histopathology in distinguishing PV from nonclonal causes
of erythrocytosis.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Teofili, M. Martini, T. Cenci, G. Petrucci, L. Torti, S. Storti, F. Guidi, G. Leone, and L. M. Larocca
Different STAT-3 and STAT-5 phosphorylation discriminates among Ph-negative chronic myeloproliferative diseases and is independent of the V617F JAK-2 mutation
Blood,
July 1, 2007;
110(1):
354 - 359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. R. Moliterno, D. M. Williams, O. Rogers, and J. L. Spivak
Molecular mimicry in the chronic myeloproliferative disorders: reciprocity between quantitative JAK2 V617F and Mpl expression
Blood,
December 1, 2006;
108(12):
3913 - 3915.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Schmitt-Graeff, J. Thiele, H. M. Kvasnicka, U. Germing, M. Luebbert, C. Waller, S. Haxelmanns, R. Nitschke, H. L. Pahl, and J. L. Spivak
Visualization of c-Mpl Expression in Ph-Negative Myeloproliferative Diseases and So-Called Essential Thrombocythemia with Ringed Sideroblasts by Confocal Laser Scanning Microscopy.
Blood (ASH Annual Meeting Abstracts),
November 16, 2004;
104(11):
2440 - 2440.
[Abstract]
|
|
|
|
|
|
|
A. Tefferi, T. L. Lasho, A. P. Wolanskyj, and R. A. Mesa
Neutrophil PRV-1 expression across the chronic myeloproliferative disorders and in secondary or spurious polycythemia
Blood,
May 1, 2004;
103(9):
3547 - 3548.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Teofili, F. Pierconti, A. Di Febo, N. Maggiano, N. Vianelli, S. Ascani, E. Rossi, S. Pileri, G. Leone, L. M. Larocca, et al.
The expression pattern of c-mpl in megakaryocytes correlates with thrombotic risk in essential thrombocythemia
Blood,
June 28, 2002;
100(2):
714 - 717.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. A. Mesa, C. A. Hanson, C.-Y. Li, S.-Y. Yoon, S. V. Rajkumar, G. Schroeder, and A. Tefferi
Diagnostic and prognostic value of bone marrow angiogenesis and megakaryocyte c-Mpl expression in essential thrombocythemia
Blood,
May 13, 2002;
99(11):
4131 - 4137.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. De Stefano, L. Teofili, G. Leone, H. L. A. Janssen, and F. R. Rosendaal
Acquired and inherited risk factors for splanchnic venous thrombosis
Blood,
May 15, 2001;
97(10):
3314 - 3316.
[Full Text]
[PDF]
|
|
|
| |
