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Blood, Vol. 96 No. 3 (August 1), 2000:
pp. 1100-1105
LSP1 modulates the locomotion of monocyte-differentiated U937
cells
Yao Li,
Qihong Zhang,
Rosemary Aaron,
Lee Hilliard, and
Thomas H. Howard
From the Department of Pediatrics, Division of Hematology/Oncology,
The University of Alabama at Birmingham, School of Medicine,
Birmingham, AL.
To examine the effect of lymphocyte specific protein 1 (LSP1) on
phagocytic cell motility, stable transfection of LSP1-null U937 cell
line with an episomal expression vector carrying the LSP1 complementary
DNA created lines expressing varied LSP1 levels. Mock transfectants
without LSP1 (U937 ) and cell lines with LSP1 levels
similar to those of monocytes (U937+) or 4-fold those of
monocytes (U937++++) express LSP1 as indicated and
express other actin-binding proteins at normal levels
before or after monocytic induction (MI) with dibutyryl
cyclic adenosine monophosphate. The cell lines were compared for rate
of growth and cell division and, after monocytic differentiation, were
video-tracked to measure locomotion as distance moved in 2 hours and
examined for morphologic changes. Rates of cell division and growth
were similar for different U937 cell lines at all LSP1 levels. In
contrast, mean rate of locomotion (micrometers moved in 2 hours) was
slower in MI-U937++++ (7.78 + 1.11µm,
n = 3) and MI-U937 (23.89 + 2.78µm, n = 3)
than in MI-U937+ cells (50.77 + 4.11µm, n = 3).
Compared with MI-U937, the locomotive histogram
(n = 150 cells) of MI-U937+ or
MI-U937++++ cells shows all cells move respectively
faster or slower as an entire cell population. In
LSP1+ U937 phagocytes, high LSP1 levels inhibit some
(locomotion) but not all (cytokinesis) cell motile behaviors and cause
the formation of surface projections. In contrast, normal LSP1 levels
in U937 phagocytes enhance some (locomotion) but not all (cytokinesis) cellular motile behaviors and have no effect on cell morphology. Therefore, LSP1 level has a unique biphasic effect on cellular locomotion. The data suggest LSP1 is an important regulator of phagocyte locomotion.
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