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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Haan, G. Articles by Van Zant, G. Search for Related Content PubMed PubMed Citation Articles by de Haan, G. Articles by Van Zant, G. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2000, Vol. 96, No. 4, pp. 1374-1379 HEMATOPOIESIS Distinct functional properties of highly purified hematopoietic stem cells from mouse strains differing in stem cell numbers Gerald de Haan, Stephen J. Szilvassy, Todd E. Meyerrose, Bert Dontje, Barry Grimes, and Gary Van Zant From the Department of Cell Biology, University of Groningen, the Netherlands; Division of Hematology/Oncology, Blood and Marrow Transplant Program, Lucille P. Markey Cancer Center, and Department of Physiology, University of Kentucky Medical Center, Lexington, KY. We have previously demonstrated that young adult DBA/2 (DBA) mice have more stem cells than C57BL/6 (B6) mice, as measured in a cobblestone area-forming cell (CAFC) assay using unfractionated marrow. To study the nature of this difference, we have now compared the proliferative fate of single, highly enriched Sca-1+c-kit+Lin stem cells from these strains. Although equal in frequency, functional comparison revealed that Sca-1+c-kit+Lin cells from DBA mice contained twice as many cells with CAFC activity. DBA clones persisted much longer in vitro, and developed later in time. To assess whether these differences were of any functional relevance in vivo, we compared engraftment of lethally irradiated mice transplanted with 1000 B6 or DBA Sca-1+c-kit+Lin cells. Recipients of enriched DBA cells recovered much faster than animals transplanted with B6 cells. We also studied endogenous hematopoietic recovery after 5-fluorouracil (5-FU) treatment in vivo. Progenitors and peripheral blood cells recovered twice as fast in DBA mice. Thus, DBA stem cells have superior proliferative potential compared with phenotypically identical stem cells obtained from B6 mice. Such genetically determined quantitative and qualitative differences in stem cell behavior likely contribute to the dramatically different hematopoietic recovery rates observed in human transplant patients. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Rezzoug, Y. Huang, M. 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