|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2000, Vol. 96, No. 4, pp. 1457-1464
IMMUNOBIOLOGY
Cloning of human early B-cell factor and identification of target
genes suggest a conserved role in B-cell development in man
and mouse
Ramiro Gisler,
Sten
Erik W. Jacobsen, and
Mikael Sigvardsson
From the Immunology Group, Cell and Molecular
Biology, Lund University; and the Stem Cell Laboratory, Lund
University Hospital, Lund, Sweden.
Early B-cell factor (EBF) is a helix-loop-helix transcription
factor suggested to be essential for B-cell development in the mouse.
Several genetic targets for EBF have been identified in mice, among
these the surrogate light chain  5 and the
signal-transducing molecules Ig (mb-1) and Ig
(B29). This article reports cloning of the human homologue
of EBF, hEBF. This protein has 93% sequence and 98.8%
amino acid homology with mouse EBF. The encoded protein binds DNA and
is expressed in cells of the B lineage, but not in cell populations
representing T lymphocytes or myeloid cells. It is also shown that
EBF-binding sites are functionally conserved in the human
mb-1 and B29 promoters because hEBF interacts
with these in the electrophoretic mobility shift assay (EMSA) and have the ability to increase the activity of reporter constructs under the
control of these promoters in nonlymphoid HeLa cells. A third genetic
target for hEBF is the promoter of the human surrogate light chain
14.1. This promoter contains 5 independent binding sites
capable of interacting with hEBF in the EMSA, and the activity of the
promoter was induced 24-fold in co-transfection experiments. These
findings suggest that the human homologue of mouse EBF displays conserved biochemical features as well as genetic targets, indicating that this protein also has an important role in human B-cell development.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. A. Jimenez, P. Akerblad, M. Sigvardsson, and E. D. Rosen
Critical Role for Ebf1 and Ebf2 in the Adipogenic Transcriptional Cascade
Mol. Cell. Biol.,
January 15, 2007;
27(2):
743 - 757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Smith and M. Sigvardsson
The roles of transcription factors in B lymphocyte commitment, development, and transformation
J. Leukoc. Biol.,
June 1, 2004;
75(6):
973 - 981.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Wang, J. W. Lewcock, P. Feinstein, P. Mombaerts, and R. R. Reed
Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection
Development,
March 15, 2004;
131(6):
1377 - 1388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-H. Wang, Z. Zhang, P. D. Burrows, H. Kubagawa, S. L. Bridges Jr, H. W. Findley, and M. D. Cooper
V(D)J recombinatorial repertoire diversification during intraclonal pro-B to B-cell differentiation
Blood,
February 1, 2003;
101(3):
1030 - 1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Liberg, M. Sigvardsson, and P. Akerblad
The EBF/Olf/Collier Family of Transcription Factors: Regulators of Differentiation in Cells Originating from All Three Embryonal Germ Layers
Mol. Cell. Biol.,
December 15, 2002;
22(24):
8389 - 8397.
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Akerblad, U. Lind, D. Liberg, K. Bamberg, and M. Sigvardsson
Early B-Cell Factor (O/E-1) Is a Promoter of Adipogenesis and Involved in Control of Genes Important for Terminal Adipocyte Differentiation
Mol. Cell. Biol.,
November 15, 2002;
22(22):
8015 - 8025.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. M. K. Smith, R. Gisler, and M. Sigvardsson
Cloning and Characterization of a Promoter Flanking the Early B Cell Factor (EBF) Gene Indicates Roles for E-Proteins and Autoregulation in the Control of EBF Expression
J. Immunol.,
July 1, 2002;
169(1):
261 - 270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Gisler and M. Sigvardsson
The Human V-PreB Promoter Is a Target for Coordinated Activation by Early B Cell Factor and E47
J. Immunol.,
May 15, 2002;
168(10):
5130 - 5138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Malone, M. D. Miner, J. R. Doerr, J. P. Jackson, S. E. Jacobsen, R. Wall, and M. Teitell
CmC(A/T)GG DNA methylation in mature B cell lymphoma gene silencing
PNAS,
August 10, 2001;
(2001)
181206898.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Malone, M. D. Miner, J. R. Doerr, J. P. Jackson, S. E. Jacobsen, R. Wall, and M. Teitell
CmC(A/T)GG DNA methylation in mature B cell lymphoma gene silencing
PNAS,
August 28, 2001;
98(18):
10404 - 10409.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
