|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 September 2000, Vol. 96, No. 5, pp. 1947-1952
NEOPLASIA
V(D)J recombinase-mediated transposition of the BCL2
gene to the IGH locus in follicular lymphoma
Jan-Willem Vaandrager,
Ed Schuuring,
Katja Philippo, and
Philip M. Kluin
From the Department of Pathology, Leiden University
Medical Center, Leiden, The Netherlands.
Using DNA fiber fluorescence in-situ hybridization
(FISH) and 3-color interphase FISH, 2 cases of follicular lymphoma were identified in which the BCL2 gene was excised from 18q21
and inserted into the immunoglobulin heavy chain (IGH)
locus at 14q32. Both the insertion breakpoint at 14q32 and the deletion
breakpoint at 18q21 were cloned using inverse polymerase chain
reaction. Sequence analysis showed that the JH sequences were
juxtaposed to the 5'-side of BCL2, and the DH sequences
were juxtaposed to the 3'-side of BCL2. There were
breakpoints at both the JH and DH recombination signal sequences, and
N-nucleotides were present at all breakpoint junctions. At the
BCL2 locus, the 3'-breakpoints in both cases were localized
at exactly the same nucleotide position, 6.2 kilobase downstream
of the major breakpoint region, directly adjacent to a complete cryptic
recombination signal sequence (RSS) consisting of a heptamer, a
nonamer, and a 23-base pair (bp) spacer. The BCL2
5'-breakpoints were approximately 600 bp upstream of the gene,
within the CA repeats. Although less evident than for the BCL2
3'-breakpoints, cryptic RSSs were also identified at these
breakpoints, with a 12-bp spacer. On the basis of structural characteristics of these rearrangements, a model is proposed in which
the BCL2 gene is deleted from its locus by recombination activation gene-1/-2 (RAG-1/-2)-mediated excision. The
gene is subsequently inserted into the recombining
IGH locus, a process involving the formation of hybrid
joints between the IGH coding ends and the
BCL2 signal ends.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Katzenberger, G. Ott, T. Klein, J. Kalla, H. K. Muller-Hermelink, and M. M. Ott
Cytogenetic Alterations Affecting BCL6 Are Predominantly Found in Follicular Lymphomas Grade 3B with a Diffuse Large B-Cell Component
Am. J. Pathol.,
August 1, 2004;
165(2):
481 - 490.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Barrans, P. A.S. Evans, S. J.M. O'Connor, R. G. Owen, G. J. Morgan, and A. S. Jack
The Detection of t(14;18) in Archival Lymph Nodes: Development of a Fluorescence in Situ Hybridization (FISH)-Based Method and Evaluation by Comparison with Polymerase Chain Reaction
J. Mol. Diagn.,
August 1, 2003;
5(3):
168 - 175.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P L Roddam, S Rollinson, M O'Driscoll, P A Jeggo, A Jack, and G J Morgan
Genetic variants of NHEJ DNA ligase IV can affect the risk of developing multiple myeloma, a tumour characterised by aberrant class switch recombination
J. Med. Genet.,
December 1, 2002;
39(12):
900 - 905.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Jiang, F. Lin, R. Price, J. Gu, L. J. Medeiros, H. Z. Zhang, S.-S. Xie, N. P. Caraway, and R. L. Katz
Rapid Detection of IgH/BCL2 Rearrangement in Follicular Lymphoma by Interphase Fluorescence in Situ Hybridization with Bacterial Artificial Chromosome Probes
J. Mol. Diagn.,
August 1, 2002;
4(3):
144 - 149.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Ott, T. Katzenberger, A. Lohr, S. Kindelberger, T. Rudiger, M. Wilhelm, J. Kalla, A. Rosenwald, J. G. Muller, M. M. Ott, et al.
Cytomorphologic, immunohistochemical, and cytogenetic profiles of follicular lymphoma: 2 types of follicular lymphoma grade 3
Blood,
May 15, 2002;
99(10):
3806 - 3812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Albinger-Hegyi, B. Hochreutener, M.-T. Abdou, I. Hegyi, M. T. Dours-Zimmermann, M. O. Kurrer, P. U. Heitz, and D. R. Zimmermann
High Frequency of t(14;18)-Translocation Breakpoints Outside of Major Breakpoint and Minor Cluster Regions in Follicular Lymphomas : Improved Polymerase Chain Reaction Protocols for Their Detection
Am. J. Pathol.,
March 1, 2002;
160(3):
823 - 832.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. L. Fenton, J.-W. Vaandrager, W. M. Aarts, R. J. Bende, K. Heering, M. van Dijk, G. Morgan, C. J. M. van Noesel, E. Schuuring, and P. M. Kluin
Follicular lymphoma with a novel t(14;18) breakpoint involving the immunoglobulin heavy chain switch mu region indicates an origin from germinal center B cells
Blood,
January 15, 2002;
99(2):
716 - 718.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Marculescu, T. Le, P. Simon, U. Jaeger, and B. Nadel
V(D)J-mediated Translocations in Lymphoid Neoplasms: A Functional Assessment of Genomic Instability by Cryptic Sites
J. Exp. Med.,
January 7, 2002;
195(1):
85 - 98.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
