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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Storms, R. W. Articles by Smith, C. Search for Related Content PubMed PubMed Citation Articles by Storms, R. W. Articles by Smith, C. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 September 2000, Vol. 96, No. 6, pp. 2125-2133 HEMATOPOIESIS Hoechst dye efflux reveals a novel CD7+CD34 lymphoid progenitor in human umbilical cord blood Robert W. Storms, Margaret A. Goodell, Alan Fisher, Richard C. Mulligan, and Clay Smith From the Center for Genetic and Cellular Therapies, Division of Experimental Surgery, Department of Surgery, Duke University Medical Center; the Duke Comprehensive Cancer Center, Duke University Medical Center; and the Division of Oncology and Transplantation; Duke University Medical Center, Durham, NC; and the Gene Therapy Program, Children's Hospital, Harvard Medical School, Boston, MA. A novel Hoechst 33342 dye efflux assay was recently developed that identifies a population of hematopoietic cells termed side population (SP) cells. In the bone marrow of multiple species, including mice and primates, the SP is composed primarily of CD34 cells, yet has many of the functional properties of hematopoietic stem cells (HSCs). This report characterizes SP cells from human umbilical cord blood (UCB). The SP in unfractionated UCB was enriched for CD34+ cells but also contained a large population of CD34 cells, many of which were mature lymphocytes. SP cells isolated from UCB that had been depleted of lineage-committed cells (Lin UCB) contained CD34+ and CD34 cells in approximately equivalent proportions. Similar to previous descriptions of human HSCs, the CD34+Lin SP cells were CD38dimHLA-DRdimThy-1dimCD45RACD71 and were enriched for myelo-erythroid precursors. In contrast, the CD34Lin SP cells were CD38HLA-DRThy-1CD71 and failed to generate myelo-erythroid progeny in vitro. The majority of these cells were CD7+CD11b+CD45RA+, as might be expected of early lymphoid cells, but did not express other lymphoid markers. The CD7+CD34Lin UCB SP cells did not proliferate in simple suspension cultures but did differentiate into natural killer cells when cultured on stroma with various cytokines. In conclusion, the human Lin UCB SP contains both CD34+ multipotential stem cells and a novel CD7+CD34Lin lymphoid progenitor. This observation adds to the growing body of evidence that CD34 progenitors exist in humans. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Moshaver, A. van Rhenen, A. Kelder, M. van der Pol, M. Terwijn, C. Bachas, A. H. Westra, G. J. Ossenkoppele, S. Zweegman, and G. J. Schuurhuis Identification of a Small Subpopulation of Candidate Leukemia-Initiating Cells in the Side Population of Patients with Acute Myeloid Leukemia Stem Cells, December 1, 2008; 26(12): 3059 - 3067. [Abstract] [Full Text] [PDF] B. Grzywacz, N. Kataria, M. Sikora, R. A. Oostendorp, E. A. Dzierzak, B. R. Blazar, J. S. Miller, and M. R. Verneris Coordinated acquisition of inhibitory and activating receptors and functional properties by developing human natural killer cells Blood, December 1, 2006; 108(12): 3824 - 3833. [Abstract] [Full Text] [PDF] M. Fischer, M. Schmidt, S. 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