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Blood, 15 September 2000, Vol. 96, No. 6, pp. 2163-2171
IMMUNOBIOLOGY
Interleukin-9 enhances interleukin-5 receptor expression,
differentiation, and survival of human eosinophils
Abdelilah Soussi Gounni,
Bernard Gregory,
Esra Nutku,
Fadi Aris,
Koussih Latifa,
Eleanor Minshall,
Janet North,
Jan Tavernier,
Roy Levit,
Nick Nicolaides,
Douglas Robinson, and
Qutayba Hamid
From Meakins-Christie Laboratories,
McGill University, Montreal, Quebec, Canada; the Department of
Allergy and Clinical Immunology, Imperial College School of Medicine,
National Heart and Lung Institute, London, England; the Department
of Medical Protein Chemistry, Flanders InterUniversity Institute
for Biotechnology, Ghent, Belgium; and Magainin Pharmaceuticals,
Plymouth Meeting, PA.
Interleukin-9 (IL-9) has been implicated in the pathogenesis
of allergic disorders. To examine the interaction between IL-9 and
eosinophils, we evaluated mature peripheral blood eosinophils for their
expression of the specific  -subunit of the IL-9 receptor (IL-9R-). The expression of IL-9R- by human eosinophils was detected at the messenger RNA (mRNA) and protein levels by reverse transcriptase-polymerase chain reaction (RT-PCR), flow cytometry, and immunocytochemical analysis, respectively. Functional analyses demonstrated that recombinant human (rh)IL-9 inhibited in vitro peripheral blood human eosinophil apoptosis in a
concentration-dependent manner. We then examined the role of IL-9 in
eosinophil differentiation using the human cord blood CD34+
cells and human promyelocytic leukemia cells (HL-60). The
addition of IL-9 to CD34+ cells cultured in IL-3 and IL-5
enhanced eosinophil development, and IL-9 alone induced
the expression of IL-5R-. IL-9 also up-regulated the
IL-5R- chain cell surface expression during terminal
eosinophil differentiation of the HL-60 cell line. Our findings suggest
that IL-9 may potentiate in vivo eosinophil function by increasing their survival and IL-5-mediated differentiation and
maturation. Taken together, these results suggest a mechanism by
which IL-9 potentiates airway and tissue eosinophilia.
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