|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 October 2000, Vol. 96, No. 7, pp. 2440-2450
HEMATOPOIESIS
Biologic significance of GATA-1 activities in Ras-mediated
megakaryocytic differentiation of hematopoietic cell lines
Itaru Matsumura,
Akira Kawasaki,
Hirokazu Tanaka,
Junko Sonoyama,
Sachiko Ezoe,
Naoko Minegishi,
Koichi Nakajima,
Masayuki Yamamoto, and
Yuzuru Kanakura
From the Department of Hematology/Oncology, Osaka
University Medical School, Osaka; Center for TARA and Institute of
Basic Medical Institute, University of Tsukuba, Tsukuba; and Department
of Immunology, Osaka City University Medical School, Osaka, Japan.
Lineage-specific transcription factors play crucial roles in
the development of hematopoietic cells. In a previous study, it was
demonstrated that Ras activation was involved in
thrombopoietin-induced megakaryocytic differentiation. In this study,
constitutive Ras activation by H-rasG12V evoked
megakaryocytic maturation of erythroleukemia cell lines F-36P and K562,
but not of myeloid cell line 32D cl3 that lacks GATA-1. However, the
introduction of GATA-1 led to reprogramming of 32D cl3 toward
erythrocytic/megakaryocytic lineage and enabled it to undergo
megakaryocytic differentiation in response to H-rasG12V. In
contrast, the overexpression of PU.1 and c-Myb changed the phenotype of
K562 from erythroid to myeloid/monocytic lineage and rendered K562 to
differentiate into granulocytes and macrophages in response to
H-rasG12V, respectively. In GATA-1-transfected 32D cl3,
the endogenous expression of PU.1 and c-Myb was easily detectable, but
their activities were reduced severely. Endogenous GATA-1 activities were markedly suppressed in PU.1-transfected and c-myb-transfected K562. As for the mechanisms of these reciprocal inhibitions,
GATA-1 and PU.1 were found to associate through their DNA-binding
domains and to inhibit the respective DNA-binding activities of
each other. In addition, c-Myb bound to GATA-1 and inhibited
its DNA-binding activities. Mutant GATA-1 and PU.1 that retained their
own transcriptional activities but could not inhibit the reciprocal
partner were less effective in changing the lineage phenotype of 32D
cl3 and K562. These results suggested that GATA-1 activities may be
crucial for Ras-mediated megakaryocytic differentiation and that its
activities may be regulated by the direct interaction with other
lineage-specific transcription factors such as PU.1 and c-Myb.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
T. Ito, C. Nishiyama, N. Nakano, M. Nishiyama, Y. Usui, K. Takeda, S. Kanada, K. Fukuyama, H. Akiba, T. Tokura, et al.
Roles of PU.1 in monocyte- and mast cell-specific gene regulation: PU.1 transactivates CIITA pIV in cooperation with IFN-{gamma}
Int. Immunol.,
July 1, 2009;
21(7):
803 - 816.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fukushima, I. Matsumura, S. Ezoe, M. Tokunaga, M. Yasumi, Y. Satoh, H. Shibayama, H. Tanaka, A. Iwama, and Y. Kanakura
FIP1L1-PDGFR{alpha} Imposes Eosinophil Lineage Commitment on Hematopoietic Stem/Progenitor Cells
J. Biol. Chem.,
March 20, 2009;
284(12):
7719 - 7732.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kitajima, M. Tanaka, J. Zheng, H. Yen, A. Sato, D. Sugiyama, H. Umehara, E. Sakai, and T. Nakano
Redirecting differentiation of hematopoietic progenitors by a transcription factor, GATA-2
Blood,
March 1, 2006;
107(5):
1857 - 1863.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ezoe, I. Matsumura, K. Gale, Y. Satoh, J. Ishikawa, M. Mizuki, S. Takahashi, N. Minegishi, K. Nakajima, M. Yamamoto, et al.
GATA Transcription Factors Inhibit Cytokine-dependent Growth and Survival of a Hematopoietic Cell Line through the Inhibition of STAT3 Activity
J. Biol. Chem.,
April 1, 2005;
280(13):
13163 - 13170.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ishiko, I. Matsumura, S. Ezoe, K. Gale, J. Ishiko, Y. Satoh, H. Tanaka, H. Shibayama, M. Mizuki, T. Era, et al.
Notch Signals Inhibit the Development of Erythroid/Megakaryocytic Cells by Suppressing GATA-1 Activity through the Induction of HES1
J. Biol. Chem.,
February 11, 2005;
280(6):
4929 - 4939.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Passioura, A. Dolnikov, S. Shen, and G. Symonds
N-Ras-Induced Growth Suppression of Myeloid Cells Is Mediated by IRF-1
Cancer Res.,
February 1, 2005;
65(3):
797 - 804.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Sevinsky, A. M. Whalen, and N. G. Ahn
Extracellular Signal-Regulated Kinase Induces the Megakaryocyte GPIIb/CD41 Gene through MafB/Kreisler
Mol. Cell. Biol.,
May 15, 2004;
24(10):
4534 - 4545.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Rekhtman, K. S. Choe, I. Matushansky, S. Murray, T. Stopka, and A. I. Skoultchi
PU.1 and pRB Interact and Cooperate To Repress GATA-1 and Block Erythroid Differentiation
Mol. Cell. Biol.,
November 1, 2003;
23(21):
7460 - 7474.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Mizuki, J. Schwable, C. Steur, C. Choudhary, S. Agrawal, B. Sargin, B. Steffen, I. Matsumura, Y. Kanakura, F. D. Bohmer, et al.
Suppression of myeloid transcription factors and induction of STAT response genes by AML-specific Flt3 mutations
Blood,
April 15, 2003;
101(8):
3164 - 3173.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Starck, N. Cohet, C. Gonnet, S. Sarrazin, Z. Doubeikovskaia, A. Doubeikovski, A. Verger, M. Duterque-Coquillaud, and F. Morle
Functional Cross-Antagonism between Transcription Factors FLI-1 and EKLF
Mol. Cell. Biol.,
February 15, 2003;
23(4):
1390 - 1402.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. L. Darley, L. Pearn, N. Omidvar, M. Sweeney, J. Fisher, S. Phillips, T. Hoy, and A. K. Burnett
Protein kinase C mediates mutant N-Ras-induced developmental abnormalities in normal human erythroid cells
Blood,
December 1, 2002;
100(12):
4185 - 4192.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ezoe, I. Matsumura, S. Nakata, K. Gale, K. Ishihara, N. Minegishi, T. Machii, T. Kitamura, M. Yamamoto, T. Enver, et al.
GATA-2/estrogen receptor chimera regulates cytokine-dependent growth of hematopoietic cells through accumulation of p21WAF1 and p27Kip1 proteins
Blood,
November 15, 2002;
100(10):
3512 - 3520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Hong, A. Y. Kim, S. Ky, C. Rakowski, S.-B. Seo, D. Chakravarti, M. Atchison, and G. A. Blobel
Inhibition of CBP-Mediated Protein Acetylation by the Ets Family Oncoprotein PU.1
Mol. Cell. Biol.,
June 1, 2002;
22(11):
3729 - 3743.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Nishiyama, M. Hasegawa, M. Nishiyama, K. Takahashi, Y. Akizawa, T. Yokota, K. Okumura, H. Ogawa, and C. Ra
Regulation of Human Fc{epsilon}RI {alpha}-Chain Gene Expression by Multiple Transcription Factors
J. Immunol.,
May 1, 2002;
168(9):
4546 - 4552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Sonoyama, I. Matsumura, S. Ezoe, Y. Satoh, X. Zhang, Y. Kataoka, E. Takai, M. Mizuki, T. Machii, H. Wakao, et al.
Functional Cooperation among Ras, STAT5, and Phosphatidylinositol 3-Kinase Is Required for Full Oncogenic Activities of BCR/ABL in K562 Cells
J. Biol. Chem.,
March 1, 2002;
277(10):
8076 - 8082.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Kumano, S. Chiba, K. Shimizu, T. Yamagata, N. Hosoya, T. Saito, T. Takahashi, Y. Hamada, and H. Hirai
Notch1 inhibits differentiation of hematopoietic cells by sustaining GATA-2 expression
Blood,
December 1, 2001;
98(12):
3283 - 3289.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Kawasaki, I. Matsumura, J.-i. Miyagawa, S. Ezoe, H. Tanaka, Y. Terada, M. Tatsuka, T. Machii, H. Miyazaki, Y. Furukawa, et al.
Downregulation of an AIM-1 Kinase Couples with Megakaryocytic Polyploidization of Human Hematopoietic Cells
J. Cell Biol.,
January 16, 2001;
152(2):
275 - 288.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
