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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Keller, S. A. Articles by Cesarman, E. Search for Related Content PubMed PubMed Citation Articles by Keller, S. A. Articles by Cesarman, E. Related Collections Neoplasia Apoptosis Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 October 2000, Vol. 96, No. 7, pp. 2537-2542 NEOPLASIA Inhibition of NF-B induces apoptosis of KSHV-infected primary effusion lymphoma cells Shannon A. Keller, Elaine J. Schattner, and Ethel Cesarman From the Weill Graduate School of Medical Sciences of Cornell University, and the Departments of Medicine and Pathology of Weill Medical College of Cornell University, New York, NY. Kaposi sarcoma-associated herpesvirus (KSHV), or human herpervirus 8 (HHV-8), is a -herpesvirus that infects human lymphocytes and is associated with primary effusion lymphoma (PEL). Currently, the role of viral infection in the transformation of PEL cells is unknown. One possibility is that KSHV, like the lymphotropic viruses Epstein-Barr virus (EBV) and human T-cell leukemia virus I (HTLV-I), activates the transcription factor NF-B to promote survival and proliferation of infected lymphocytes. To examine this possibility, we assessed NF-B activity in KSHV-infected PEL cell lines and primary tumor specimens by electrophoretic mobility shift assay (EMSA). We observed that NF-B is constitutively activated in all KSHV-infected lymphomas, and consists of 2 predominant complexes, p65/p50 heterodimers and p50/p50 homodimers. Inhibition experiments demonstrated that Bay 11-7082, an irreversible inhibitor of IB phosphorylation, completely and specifically abrogated the NF-B/DNA binding in PEL cells. PEL cells treated with Bay 11 demonstrated down-regulation of the NF-B inducible cytokine interleukin 6 (IL-6), and apoptosis. These results suggest that NF-B activity is necessary for survival of KSHV-infected lymphoma cells, and that pharmacologic inhibition of NF-B may be an effective treatment for PEL. © 2000 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. L. Di Bartolo, M. Cannon, Y.-F. Liu, R. Renne, A. Chadburn, C. Boshoff, and E. 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