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Blood, 15 October 2000, Vol. 96, No. 8, pp. 2740-2745
HEMATOPOIESIS
Expression of the collagen receptor glycoprotein VI during
megakaryocyte differentiation
Oscar Berlanga,
Regis Bobe,
Marion Becker,
George Murphy,
Mireille Leduc,
Cassian Bon,
Fiona A. Barry,
Jonathan M. Gibbins,
Paloma Garcia,
Jonathan Frampton, and
Stephen
P. Watson
From the Department of Pharmacology, University of
Oxford, United Kingdom; Institute of Molecular Medicine, John Radcliffe
Hospital, Oxford, United Kingdom; Unité des venins, Institut
Pasteur, Paris, France; School of Animal and Microbial Sciences,
University of Reading, Reading, United Kingdom.
This study examined the expression of the platelet collagen
receptor glycoprotein VI (GPVI) in megakaryocyte cell lines and primary megakaryocytes by reverse transcriptase-polymerase chain reaction and by flow cytometry and ligand blotting using the
snake venom toxin convulxin. Expression of GPVI is increased in the megakaryoblastic cell lines HEL and CMK on differentiation with the
phorbol ester phorbol 12-myristate 13-acetate (PMA), along with the Fc
receptor  -chain (FcR -chain). The increase in GPVI expression is associated with marked potentiation of tyrosine phosphorylation and Ca++ elevation in response to
convulxin. Syk, linker for activated T cells, and phospholipase C2
(PLC2) are among the proteins tyrosine phosphorylated on convulxin
stimulation in PMA-differentiated HEL cells. Studies on primary murine
megakaryocytes grown in vitro confirmed that GPVI is up-regulated in
parallel with functional activation, assessed by measurement of
[Ca++]i, during differentiation. The results
demonstrate that expression of GPVI is up-regulated along with
the FcR -chain during differentiation of megakaryocytes.
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