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Blood, 1 November 2000, Vol. 96, No. 9, pp. 3272-3275
BRIEF REPORT
Assessment of bone marrow stem cell reserve and function and
stromal cell function in patients with autoimmune cytopenias
Helen A. Papadaki,
Frances
M. Gibson,
Sian Rizzo,
Edward C. Gordon-Smith, and
Judith C. W. Marsh
From the Department of Hematology, St George's
Hospital Medical School, London, United Kingdom.
To investigate whether bone marrow (BM) stem cell compartment
and/or BM microenvironment are affected by the immune insult in
autoimmune cytopenias (AICs), BM stem cell reserve and function and BM
stromal function were studied in 15 AIC patients. Stem cells
were evaluated by means of flow cytometry, clonogenic progenitor cell
assays, long-term BM cultures (LTBMCs), and limiting dilution assay for
quantification of long-term-culture initiating cells (LTC-ICs).
Stromal cell function was assessed with the use of preformed irradiated
LTBMCs from patients and normal controls, recharged with normal
CD34+ cells. AIC patients exhibited a high number of
CD34+, CD34+/CD38+, and
CD34+/CD38 cells; high frequency of
granulocyte-macrophage colony forming units in the BM mononuclear
cell fraction; high colony recovery in LTBMCs; and normal
LTC-IC frequency. Patient BM stromal layers displayed normal
hematopoietic-supporting capacity and increased production of
granulocyte-colony stimulating factor. Data from this study support the
concept that AIC patients with severe, resistant disease might be
appropriate candidates for autologous stem cell transplantation.
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