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Blood, 1 January 2001, Vol. 97, No. 1, pp. 288-296
PHAGOCYTES
Characterization of human sialoadhesin, a sialic acid binding
receptor expressed by resident and inflammatory macrophage
populations
Adele Hartnell,
Jane Steel,
Helen Turley,
Margaret Jones,
David G. Jackson, and
Paul R. Crocker
From the Imperial Cancer Research Fund Laboratories;
the Department of Cellular Science; and the Nuffield Department of
Medicine, University of Oxford, Institute of Molecular Medicine, John
Radcliffe Hospital, Oxford, United Kingdom; Imperial Cancer Research
Fund, London, United Kingdom; and Wellcome Trust Biocentre, Department
of Biochemistry, University of Dundee, Dundee, Scotland.
Sialoadhesin is a macrophage-restricted cellular interaction
molecule and a prototypic member of the Siglec family of sialic acid
binding immunoglobulin (Ig)-like lectins. So far, it has only
been characterized in rodents. Here, we report the molecular cloning,
binding properties, and expression pattern of human sialoadhesin. The
predicted protein sequences of human and mouse sialoadhesin are
about 72% identical, with the greatest similarity in the
extracellular region, which comprises 17 Ig domains in both species. A
recombinant protein consisting of the first 4 N-terminal domains of
human sialoadhesin fused to the Fc region of human IgG1
mediated sialic acid-dependent binding with a specificity similar to
its mouse counterpart, preferring sialic acid in the  2,3 glycosidic
linkage over the 2,6 linkage. By flow cytometry with
peripheral blood leukocytes, recombinant sialoadhesin bound
strongly to granulocytes with intermediate binding to monocytes,
natural killer cells, B cells, and a subset of CD8 T cells. Using
antibodies raised to the recombinant protein, sialoadhesin
was immunoprecipitated from the THP-1 human monocytic cell line as an
approximate 200-kd glycoprotein. The expression pattern of human
sialoadhesin was found to be similar to that of the mouse receptor,
being absent from monocytes and other peripheral blood leukocytes, but
expressed strongly by tissue macrophages in the spleen, lymph node,
bone marrow, liver, colon, and lungs. High expression was also found on
inflammatory macrophages present in affected tissues from patients with
rheumatoid arthritis.
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