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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ciric, B. Articles by Pease, L. R. Search for Related Content PubMed PubMed Citation Articles by Ciric, B. Articles by Pease, L. R. Related Collections Immunobiology Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 January 2001, Vol. 97, No. 1, pp. 321-323 BRIEF REPORT Clonal evolution in Waldenstrom macroglobulinemia highlights functional role of B-cell receptor Bogoljub Ciric, Virginia VanKeulen, Moses Rodriguez, Robert A. Kyle, Morie A. Gertz, and Larry R. Pease From the Department of Immunology, Mayo Medical and Graduate School, Rochester; Division of Hematology and Internal Medicine, Mayo Clinic, Rochester, MN. The course of clonal evolution of 2 related clones in the blood of a patient with Waldenstrom macroglobulinemia (WM) indicates the functional importance for the expression of the B-cell receptor for the survival of these malignant cells. Protein and nucleotide sequencing of the paraproteins' variable regions revealed 2 predominant V and 2 VH sequences, each set comprised in the ratio 1:1.5. The 2 VH sequences and 2 V sequences shared the same VDJ and VJ junctional sequences, respectively, indicating that 2 malignant clones had evolved from a common ancestor. This is the first report on intraclonal heterogeneity in WM. Comparison of the V and VH sequences with the closest matching known germline genes showed that they contained approximately 10 somatic mutations each. The distribution and type of mutations demonstrate that mutations have continued to accumulate in the malignant clones and that selection has been operating to preserve immunoglobulin structure. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: P. Martin-Jimenez, R. Garcia-Sanz, A. Balanzategui, M. Alcoceba, E. Ocio, M{a} L. Sanchez, M. Gonzalez, and J. San Miguel Molecular characterization of heavy chain immunoglobulin gene rearrangements in Waldenstrom's macroglobulinemia and IgM monoclonal gammopathy of undetermined significance Haematologica, May 1, 2007; 92(5): 635 - 642. [Abstract] [Full Text] [PDF] J. Kriangkum, B. J. Taylor, S. P. Treon, M. J. Mant, T. Reiman, A. R. Belch, and L. M. Pilarski Molecular Characterization of Waldenstrom's Macroglobulinemia Reveals Frequent Occurrence of Two B-Cell Clones Having Distinct IgH VDJ Sequences Clin. Cancer Res., April 1, 2007; 13(7): 2005 - 2013. [Abstract] [Full Text] [PDF] M. R. Murali, A. Kratz, and K. E. Finberg Case 40-2006 -- A 64-Year-Old Man with Anemia and a Low Level of HDL Cholesterol N. Engl. J. Med., December 28, 2006; 355(26): 2772 - 2779. [Full Text] [PDF] J. Kriangkum, B. J. Taylor, S. P. Treon, M. J. Mant, A. R. Belch, and L. M. Pilarski Clonotypic IgM V/D/J sequence analysis in Waldenstrom macroglobulinemia suggests an unusual B-cell origin and an expansion of polyclonal B cells in peripheral blood Blood, October 1, 2004; 104(7): 2134 - 2142. [Abstract] [Full Text] [PDF] S. S. Sahota, F. Forconi, C. H. Ottensmeier, D. Provan, D. G. Oscier, T. J. Hamblin, and F. K. Stevenson Typical Waldenstrom macroglobulinemia is derived from a B-cell arrested after cessation of somatic mutation but prior to isotype switch events Blood, July 30, 2002; 100(4): 1505 - 1507. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020