|
|
Previous Article | Table of Contents | Next Article 
Blood, 1 June 2001, Vol. 97, No. 11, pp. 3433-3440
HEMATOPOIESIS
Chemotaxis and transendothelial migration of CD34+
hematopoietic progenitor cells induced by the inflammatory mediator
leukotriene D4 are mediated by the 7-transmembrane
receptor CysLT1
Frank Bautz,
Claudio Denzlinger,
Lothar Kanz, and
Robert Möhle
From the Department of Medicine II, University of
Tübingen, Germany.
Recent studies suggest that bone marrow (BM)-derived chemotactic
mediators such as chemokines play key roles in hematopoietic stem cell
trafficking. Lipid mediators, particularly leukotrienes, are involved
in leukocyte chemotaxis during inflammation but have also been detected
in the normal BM. Therefore, the effects of leukotrienes on
hematopoietic progenitor cells were analyzed. Cysteinyl leukotrienes,
particularly leukotriene D4 (LTD4), induced strong intracellular
calcium fluxes and actin polymerization in mobilized and BM
CD34+ progenitors. Chemotaxis and in vitro transendothelial
migration of CD34+ and more primitive
CD34+/CD38 cells were 2-fold increased by
LTD4 at an optimum concentration of 25 to 50 nM. Accordingly,
CD34+ cells expressed the 7-transmembrane LTD4 receptor
CysLT1 by reverse transcriptase-polymerase chain reaction and Western
blot. Effects of LTD4 were suppressed by the CysLT1 receptor antagonist
MK-571 and reduced by pertussis toxin. In contrast, LTB4 induced strong responses only in mature granulocytes. LTD4-induced calcium fluxes were
also observed in granulocytes but were not reduced by MK-571, suggesting that these effects were mediated by other receptors (eg,
CysLT2) rather than by CysLT1. In addition, expression of 5-lipoxygenase, the key enzyme of leukotriene biosynthesis, was detected in both hematopoietic progenitor cells and mature leukocytes. The study concludes that the functionally active LTD4 receptor CysLT1
is preferentially expressed in immature hematopoietic progenitor cells.
LTD4 released in the BM might regulate progenitor cell trafficking and
could also act as an autocrine mediator of hematopoiesis. This would be
a first physiologic effect of cysteinyl leukotrienes apart
from the many known pathophysiologic actions related to allergy and inflammation.

CiteULike Connotea Del.icio.us Digg Reddit Technorati What's this?
This article has been cited by other articles:

|
 |

|
 |
 
A. M. Boehmler, A. Drost, L. Jaggy, G. Seitz, T. Wiesner, C. Denzlinger, L. Kanz, and R. Mohle
The CysLT1 Ligand Leukotriene D4 Supports {alpha}4{beta}1- and {alpha}5{beta}1-Mediated Adhesion and Proliferation of CD34+ Hematopoietic Progenitor Cells
J. Immunol.,
June 1, 2009;
182(11):
6789 - 6798.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y.-S. Maeng, H.-J. Choi, J.-Y. Kwon, Y.-W. Park, K.-S. Choi, J.-K. Min, Y.-H. Kim, P.-G. Suh, K.-S. Kang, M.-H. Won, et al.
Endothelial progenitor cell homing: prominent role of the IGF2-IGF2R-PLC{beta}2 axis
Blood,
January 1, 2009;
113(1):
233 - 243.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Tabatabai, C. Herrmann, G. von Kurthy, M. Mittelbronn, S. Grau, B. Frank, R. Mohle, M. Weller, and W. Wick
VEGF-dependent induction of CD62E on endothelial cells mediates glioma tropism of adult haematopoietic progenitor cells
Brain,
October 1, 2008;
131(10):
2579 - 2595.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
R. M. Lemoli and A. D'Addio
Hematopoietic stem cell mobilization
Haematologica,
March 1, 2008;
93(3):
321 - 324.
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
L. Rossi, R. Manfredini, F. Bertolini, D. Ferrari, M. Fogli, R. Zini, S. Salati, V. Salvestrini, S. Gulinelli, E. Adinolfi, et al.
The extracellular nucleotide UTP is a potent inducer of hematopoietic stem cell migration
Blood,
January 15, 2007;
109(2):
533 - 542.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Tabatabai, B. Frank, R. Mohle, M. Weller, and W. Wick
Irradiation and hypoxia promote homing of haematopoietic progenitor cells towards gliomas by TGF-{beta}-dependent HIF-1{alpha}-mediated induction of CXCL12
Brain,
September 1, 2006;
129(9):
2426 - 2435.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
G. Tabatabai, O. Bahr, R. Mohle, I. Y. Eyupoglu, A. M. Boehmler, J. Wischhusen, J. Rieger, I. Blumcke, M. Weller, and W. Wick
Lessons from the bone marrow: how malignant glioma cells attract adult haematopoietic progenitor cells
Brain,
September 1, 2005;
128(9):
2200 - 2211.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
I. Prinz, C. Gregoire, H. Mollenkopf, E. Aguado, Y. Wang, M. Malissen, S. H.E. Kaufmann, and B. Malissen
The Type 1 Cysteinyl Leukotriene Receptor Triggers Calcium Influx and Chemotaxis in Mouse {alpha}{beta}- and {gamma}{delta} Effector T Cells
J. Immunol.,
July 15, 2005;
175(2):
713 - 719.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
Y. Kanaoka and J. A. Boyce
Cysteinyl Leukotrienes and Their Receptors: Cellular Distribution and Function in Immune and Inflammatory Responses
J. Immunol.,
August 1, 2004;
173(3):
1503 - 1510.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
T. Kimura, A. M. Boehmler, G. Seitz, S. Kuci, T. Wiesner, V. Brinkmann, L. Kanz, and R. Mohle
The sphingosine 1-phosphate receptor agonist FTY720 supports CXCR4-dependent migration and bone marrow homing of human CD34+ progenitor cells
Blood,
June 15, 2004;
103(12):
4478 - 4486.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Parameswaran, R. Watson, G. M. Gauvreau, R. Sehmi, and P. M. O'Byrne
The Effect of Pranlukast on Allergen-induced Bone Marrow Eosinophilopoiesis in Subjects with Asthma
Am. J. Respir. Crit. Care Med.,
April 15, 2004;
169(8):
915 - 920.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
K. Chibana, Y. Ishii, T. Asakura, and T. Fukuda
Up-Regulation of Cysteinyl Leukotriene 1 Receptor by IL-13 Enables Human Lung Fibroblasts to Respond to Leukotriene C4 and Produce Eotaxin
J. Immunol.,
April 15, 2003;
170(8):
4290 - 4295.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
E. A. Mellor, K. F. Austen, and J. A. Boyce
Cysteinyl Leukotrienes and Uridine Diphosphate Induce Cytokine Generation by Human Mast Cells Through an Interleukin 4-regulated Pathway that Is Inhibited by Leukotriene Receptor Antagonists
J. Exp. Med.,
March 4, 2002;
195(5):
583 - 592.
[Abstract]
[Full Text]
[PDF]
|
 |
|

|
 |

|
 |
 
S. Fruehauf, K. Srbic, R. Seggewiss, J. Topaly, and A. D. Ho
Functional characterization of podia formation in normal and malignant hematopoietic cells
J. Leukoc. Biol.,
March 1, 2002;
71(3):
425 - 432.
[Abstract]
[Full Text]
[PDF]
|
 |
|
|
|