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Blood, 15 June 2001, Vol. 97, No. 12, pp. 3683-3690
PLENARY PAPER
Identification of a novel, human multilymphoid progenitor in
cord blood
Qian-Lin Hao,
Judy Zhu,
Mary A. Price,
Kimberly J. Payne,
Lora W. Barsky, and
Gay M. Crooks
From the Division of Research Immunology/Bone Marrow
Transplantation, Childrens Hospital of Los Angeles, CA.
The earliest stages of lymphoid commitment from human pluripotent
hematopoietic stem cells have not been defined. A clonogenic subpopulation of CD34+CD38 cord blood
cells were identified that expressed high levels of the CD7 antigen and
possessed only lymphoid potential.
CD34+CD38CD7+ (CD7+)
cells uniformly coexpressed CD45RA and HLA-DR;
c-kit and Thy-1 expression was absent to low.
Clonal analysis demonstrated that single CD7+ cells could
generate B cells, natural killer cells, and dendritic cells but were
devoid of myeloid or erythroid potential. In contrast, control
CD34+CD38CD7
(CD7) cells generated both lymphoid and myelo-erythroid
cells. The lymphoid potential (generation of lymphoid progeny in bulk
and single cell cultures) of CD7+ cells was equivalent to
that of the pluripotent CD7 cells. RNA expression studies
showed that CD7+ cells expressed PU.1 and GATA-3, but did
not express Pax-5, terminal deoxynucleotide transferase,
or CD3 . In contrast to the previously described murine common
lymphoid progenitor, the  chain of the receptor for interleukin-7
was not detected by fluorescence-activated cell sorting
analysis or RNA polymerase chain reaction in CD7+
cells. These studies identify a clonogenic lymphoid progenitor with
both B-cell and natural killer cell lineage potential with a molecular
profile that suggests a developmental stage more primitive than
previously identified lymphoid progenitors. The CD7+
phenotype distinguishes primitive human lymphoid progenitors from
pluripotent stem cells, thus allowing the study of regulation of
early human lymphopoiesis and providing an alternative to pluripotent stem cells for genetic manipulation and transplantation.
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