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Blood, 15 June 2001, Vol. 97, No. 12, pp. 3763-3767
HEMATOPOIESIS
Expression of the neuronal cyclin-dependent kinase 5 activator p35Nck5a in human monocytic cells is associated with
differentiation
Fei Chen and
George P. Studzinski
From the Department of Pathology and Laboratory
Medicine, UMDNJ-New Jersey Medical School, Newark.
Although cyclin-dependent kinase 5 (Cdk5) is widely expressed in
human tissues, its activator p35Nck5a is generally considered to be
neuron specific. In addition to neuronal cells, active Cdk5 complexes
have been reported in developing tissues, such as the embryonic muscle
and ocular lens, and in human leukemia HL60 cells induced to
differentiate by an exposure to 1,25-dihydroxyvitamin D3;
however, its activator in these cells has not been demonstrated. The
results of this study indicate that p35Nck5a is associated with Cdk5 in
monocytic differentiation of hematopoietic cells. Specifically,
p35Nck5a is expressed in normal human monocytes and in leukemic cells
induced to differentiate toward the monocytic lineage, but not in
lymphocytes or cells induced to granulocytic differentiation by
retinoic acid. It is present in a complex with Cdk5 that has protein
kinase activity, and when ectopically expressed together with Cdk5 in
undifferentiated HL60 cells, it induces the expression of CD14 and
"nonspecific" esterase, markers of monocytic phenotype. These
observations not only indicate a functional relationship between Cdk5
and p35Nck5a, but also support a role for this complex in monocytic differentiation.
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