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Related Collections Hemostasis, Thrombosis, and Vascular Biology Immunobiology Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 June 2001, Vol. 97, No. 12, pp. 3812-3819 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Severe impairment of leukocyte rolling in venules of core 2 glucosaminyltransferase-deficient mice Markus Sperandio, Aravinda Thatte, Dan Foy, Lesley G. Ellies, Jamey D. Marth, and Klaus Ley From the Department of Biomedical Engineering, University of Virginia, Charlottesville, and the Howard Hughes Medical Institute, Department of Cellular and Molecular Medicine, University of California, La Jolla. Leukocyte capture and rolling are mediated by selectins expressed on leukocytes (L-selectin) and the vascular endothelium (P- and E-selectin). To investigate the role of core 2 1-6-N-glucosaminyltransferase (C2GlcNAcT-I) for synthesis of functional selectin ligands in vivo, leukocyte rolling flux and velocity were studied in venules of untreated and tumor necrosis factor- (TNF)-pretreated autoperfused cremaster muscles of C2GlcNAcT-I-deficient (core 2/) and littermate control mice. In untreated core 2/ mice, leukocyte rolling was dramatically reduced with markedly increased rolling velocities (81 ± 4 µm/s vs 44 ± 3 µm/s). The reduced rolling in core 2/ mice was due mainly to severely impaired binding of P-selectin to P-selectin glycoprotein ligand-1 (PSGL-1). Some rolling remained after blocking PSGL-1 in controls but not in core 2/ mice. In TNF-pretreated mice, rolling was markedly reduced in core 2/ mice owing to impaired P-selectin- and E-selectin-mediated rolling. Rolling velocities in core 2/ mice treated with an E-selectin-blocking monoclonal antibody (59 ± 4 µm/s) were significantly higher than in controls (14 ± 1 µm/s), which provides further evidence for the severe impairment in P-selectin-mediated rolling. In conclusion, P-selectin ligands including PSGL-1 are largely C2GlcNAcT-I dependent. In addition, E-selectin-mediated rolling in vivo is partially dependent on the targeted C2GlcNAcT-I. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Wang, W. Zhang, R. Tang, R. P. Hebbel, M. A. Kowalska, C. Zhang, J. D. Marth, M. Fukuda, C. Zhu, and Y. Huo Core2 1-6-N-Glucosaminyltransferase-I Deficiency Protects Injured Arteries From Neointima Formation in ApoE-Deficient Mice Arterioscler Thromb Vasc Biol, July 1, 2009; 29(7): 1053 - 1059. [Abstract] [Full Text] [PDF] H. Wang, R. Tang, W. Zhang, K. Amirikian, Z. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020