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Related Collections Hematopoiesis and Stem Cells Transplantation Cell Adhesion and Motility Chemokines, Cytokines, and Interleukins Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 February 2001, Vol. 97, No. 3, pp. 799-804 TRANSPLANTATION In vitro migratory capacity of CD34+ cells is related to hematopoietic recovery after autologous stem cell transplantation Carlijn Voermans, Marisha L. K. Kooi, Sjoerd Rodenhuis, Hans van der Lelie, C. Ellen van der Schoot, and Winald R. Gerritsen From the Divisions of Medical Oncology and Biometrics, Netherlands Cancer Institute; CLB, Sanquin Blood Supply Foundation, Laboratory for Experimental and Clinical Immunology, and Department of Hematology, Academic Medical Center, University of Amsterdam; and Department of Medical Oncology, Division of Gene Therapy Program, University Hospital of Vrije Universiteit, Amsterdam, Netherlands. To investigate whether the migratory ability of peripheral blood-derived CD34+ cells of patients undergoing autologous peripheral blood stem cell transplantation is related to the homing efficiency of these cells, the migration in vitro of these cells was determined and correlated with in vivo hematopoietic recovery. Large inter-individual differences of the in vitro migratory ability of the CD34+ cells were observed, ranging from 1.1% to 16.4% for spontaneous migration and 6.2% to 40.8% for SDF-1-induced (100 ng/mL) migration. Significantly faster hematologic recovery was observed in those patients who received transplanted CD34+ cells that showed high spontaneous and SDF-1-induced migration in vitro (P < .05). Moreover, CD34+ cells from healthy G-CSF-mobilized donors exhibited significantly higher spontaneous and SDF-1-induced (P < .01) migration than CD34+ cells from patients mobilized with chemotherapy and G-CSF. The lower migratory capacity in vitro of patient-derived CD34+ cells was not due to lower expression of CXCR-4 but probably reflected decreased motogenic behavior of the cells. These results indicate that the migratory capacity of the cells is important for hematopoietic recovery. The data suggest that the engraftment potential of autologous stem cells is more or less impaired by treatment before or during the mobilization procedure and might possibly be restored by in vitro manipulation of the cells. In addition, an exponential relation between CXCR-4 expression and number of CD34+ cells that mobilized to the peripheral blood was found (P < .001), suggesting that CXCR-4 expression plays a role in the mobilization of CD34+ cells. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Lin, E. De Stanchina, X. K. Zhou, Y. She, D. Hoang, S. W. Cheung, B. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020