|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 February 2001, Vol. 97, No. 4, pp. 1001-1008
IMMUNOBIOLOGY
Ordered recombination of immunoglobulin light chain genes
occurs at the IGK locus but seems less strict at the
IGL locus
Mirjam van der Burg,
Talip Tümkaya,
Marjan Boerma,
Sandra de
Bruin-Versteeg,
Anton W. Langerak, and
Jacques J. M. van
Dongen
From the Department of Immunology, Erasmus University
Rotterdam/ University Hospital Rotterdam, Rotterdam, The Netherlands.
Regulation of allelic and isotypic exclusion of human
immunoglobulin (Ig) light-chain genes was studied in 113 chronic B-cell leukemias as a "single-cell" model that allowed complete analysis of each light chain allele. Our data show that monospecific Ig light
chain expression is in about 90% of cases determined by ordered
recombination: Ig gene (IGK) rearrangements, followed by
IGK deletions and Ig gene (IGL)
rearrangements, resulting in the presence of only one functional Ig
light chain rearrangement. In about 10% (10 cases), 2 functional Ig
light chain rearrangements (IGK/IGL or IGL/IGL,
but not IGK/IGK) were identified. This might be explained
by the fact that regulation of the ordered recombination process is not
fully strict, particularly when the IGL locus is involved.
Unfavorable somatic mutations followed by receptor editing might have
contributed to this finding. Eight of these 10 cases indeed contained
somatic mutations. In cases with 2 functional Ig light chain
rearrangements, both alleles were transcribed, but monospecific Ig
expression was still maintained. This suggests that in these cases
allelelic exclusion is not regulated at the messenger RNA level but
either at the level of translation or protein stability or via
preferential pairing of Ig light and Ig heavy chains. Nevertheless,
ordered rearrangement processes are the main determinant for
monospecific Ig light chain expression.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
E. M. Ghia, S. Jain, G. F. Widhopf II, L. Z. Rassenti, M. J. Keating, W. G. Wierda, J. G. Gribben, J. R. Brown, K. R. Rai, J. C. Byrd, et al.
Use of IGHV3-21 in chronic lymphocytic leukemia is associated with high-risk disease and reflects antigen-driven, post-germinal center leukemogenic selection
Blood,
May 15, 2008;
111(10):
5101 - 5108.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Forconi, E. Sozzi, D. Rossi, S. S. Sahota, T. Amato, D. Raspadori, L. Trentin, L. Leoncini, G. Gaidano, and F. Lauria
Selective influences in the expressed immunoglobulin heavy and light chain gene repertoire in hairy cell leukemia
Haematologica,
May 1, 2008;
93(5):
697 - 705.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Gonzalez, M. van der Burg, R. Garcia-Sanz, J. A. Fenton, A. W. Langerak, M. Gonzalez, J. J. M. van Dongen, J. F. San Miguel, and G. J. Morgan
Immunoglobulin gene rearrangements and the pathogenesis of multiple myeloma
Blood,
November 1, 2007;
110(9):
3112 - 3121.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-G. Velez, M. Kane, S. Liu, S. B. Gauld, J. C. Cambier, R. M. Torres, and R. Pelanda
Ig Allotypic Inclusion Does Not Prevent B Cell Development or Response
J. Immunol.,
July 15, 2007;
179(2):
1049 - 1057.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. van Zelm, T. Szczepanski, M. van der Burg, and J. J.M. van Dongen
Replication history of B lymphocytes reveals homeostatic proliferation and extensive antigen-induced B cell expansion
J. Exp. Med.,
March 19, 2007;
204(3):
645 - 655.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. C. van Zelm, M. van der Burg, D. de Ridder, B. H. Barendregt, E. F. E. de Haas, M. J. T. Reinders, A. C. Lankester, T. Revesz, F. J. T. Staal, and J. J. M. van Dongen
Ig Gene Rearrangement Steps Are Initiated in Early Human Precursor B Cell Subsets and Correlate with Specific Transcription Factor Expression
J. Immunol.,
November 1, 2005;
175(9):
5912 - 5922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Liu, M.-G. Velez, J. Humann, S. Rowland, F. J. Conrad, R. Halverson, R. M. Torres, and R. Pelanda
Receptor Editing Can Lead to Allelic Inclusion and Development of B Cells That Retain Antibodies Reacting with High Avidity Autoantigens
J. Immunol.,
October 15, 2005;
175(8):
5067 - 5076.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. K. Pai, A. E. Chakerian, J. M. Binder, M. Amin, and D. S. Viswanatha
B-Cell Clonality Determination Using an Immunoglobulin {kappa} Light Chain Polymerase Chain Reaction Method
J. Mol. Diagn.,
May 1, 2005;
7(2):
300 - 307.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. W. Langerak, B. Nadel, A. de Torbal, I. L. M. Wolvers-Tettero, E. J. van Gastel-Mol, B. Verhaaf, U. Jager, and J. J. M. van Dongen
Unraveling the Consecutive Recombination Events in the Human IGK Locus
J. Immunol.,
September 15, 2004;
173(6):
3878 - 3888.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Noordzij, S. de Bruin-Versteeg, N. S. Verkaik, J. M. J. J. Vossen, R. de Groot, E. Bernatowska, A. W. Langerak, D. C. van Gent, and J. J. M. van Dongen
The immunophenotypic and immunogenotypic B-cell differentiation arrest in bone marrow of RAG-deficient SCID patients corresponds to residual recombination activities of mutated RAG proteins
Blood,
August 28, 2002;
100(6):
2145 - 2152.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. van der Burg, B. H. Barendregt, E. J. van Gastel-Mol, T. Tumkaya, A. W. Langerak, and J. J. M. van Dongen
Unraveling of the Polymorphic C{lambda}2-C{lambda}3 Amplification and the Ke+Oz- Polymorphism in the Human Ig{lambda} Locus
J. Immunol.,
July 1, 2002;
169(1):
271 - 276.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Szczepanski, M. J. Willemse, B. Brinkhof, E. R. van Wering, M. van der Burg, and J. J. M. van Dongen
Comparative analysis of Ig and TCR gene rearrangements at diagnosis and at relapse of childhood precursor-B-ALL provides improved strategies for selection of stable PCR targets for monitoring of minimal residual disease
Blood,
April 1, 2002;
99(7):
2315 - 2323.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
