|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 February 2001, Vol. 97, No. 4, pp. 1023-1026
IMMUNOBIOLOGY
VH1-69 gene is preferentially used by
hepatitis C virus-associated B cell lymphomas and by normal B cells
responding to the E2 viral antigen
Chunghuang Hubert Chan,
Kenneth G. Hadlock,
Steven K. H. Foung, and
Shoshana Levy
From the Department of Medicine, Division of Oncology
and Pathology, Stanford University Medical Center, Stanford, CA.
Hepatitis C virus (HCV)-associated B cell lymphomas were
previously shown to express a restricted repertoire of immunoglobulin VH and VL genes, VH1-69 and
V A27, respectively. Although this suggests a role for antigen
selection in the pathogenesis of these lymphomas, the driving antigen
involved in the clonal expansion has not been identified. B cell
response to a viral antigen, the HCV envelope glycoprotein 2 (E2), was
analyzed in an asymptomatic HCV-infected patient. Single B cells,
immortalized as hybridomas and selected for binding E2, were analyzed
for their V gene usage. Sequences of these V region genes demonstrated
that each hybridoma expressed unique VH and VL
genes. Remarkably, these anti-E2 hybridomas preferentially used the
VH1-69 gene. Analysis of replacement to silent mutation
ratios indicated that the genes underwent somatic mutation and
antigenic selection. In a separate report, human anti-E2 antibodies
were also shown to express the same VH gene. These data
strengthen the hypothesis that the HCV-associated lymphomas are derived
from clonally expanded B cells stimulated by HCV.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
D. Saadoun, D. A. Landau, L. H. Calabrese, and P. P. Cacoub
Hepatitis C-associated mixed cryoglobulinaemia: a crossroad between autoimmunity and lymphoproliferation
Rheumatology,
August 1, 2007;
46(8):
1234 - 1242.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Naas, M. Ghorbani, I. Alvarez-Maya, M. Lapner, R. Kothary, Y. De Repentigny, S. Gomes, L. Babiuk, A. Giulivi, C. Soare, et al.
Characterization of liver histopathology in a transgenic mouse model expressing genotype 1a hepatitis C virus core and envelope proteins 1 and 2
J. Gen. Virol.,
August 1, 2005;
86(8):
2185 - 2196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Carbonari, E. Caprini, T. Tedesco, F. Mazzetta, V. Tocco, M. Casato, G. Russo, and M. Fiorilli
Hepatitis C Virus Drives the Unconstrained Monoclonal Expansion of VH1-69-Expressing Memory B Cells in Type II Cryoglobulinemia: A Model of Infection-Driven Lymphomagenesis
J. Immunol.,
May 15, 2005;
174(10):
6532 - 6539.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Vallisa, P. Bernuzzi, L. Arcaini, S. Sacchi, V. Callea, R. Marasca, A. Lazzaro, E. Trabacchi, E. Anselmi, A. L. Arcari, et al.
Role of Anti-Hepatitis C Virus (HCV) Treatment in HCV-Related, Low-Grade, B-Cell, Non-Hodgkin's Lymphoma: A Multicenter Italian Experience
J. Clin. Oncol.,
January 20, 2005;
23(3):
468 - 473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ahmad and F. Alvarez
Role of NK and NKT cells in the immunopathogenesis of HCV-induced hepatitis
J. Leukoc. Biol.,
October 1, 2004;
76(4):
743 - 759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Y. Keck, A. Op De Beeck, K. G. Hadlock, J. Xia, T.-K. Li, J. Dubuisson, and S. K. H. Foung
Hepatitis C Virus E2 Has Three Immunogenic Domains Containing Conformational Epitopes with Distinct Properties and Biological Functions
J. Virol.,
September 1, 2004;
78(17):
9224 - 9232.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. H. Sasso, M. Martinez, S. L. Yarfitz, P. Ghillani, L. Musset, J.-C. Piette, and P. Cacoub
Frequent Joining of Bcl-2 to a JH6 Gene in Hepatitis C Virus-Associated t(14;18)
J. Immunol.,
September 1, 2004;
173(5):
3549 - 3556.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-Y. Keck, V. M. H. Sung, S. Perkins, J. Rowe, S. Paul, T. J. Liang, M. M. C. Lai, and S. K. H. Foung
Human Monoclonal Antibody to Hepatitis C Virus E1 Glycoprotein That Blocks Virus Attachment and Viral Infectivity
J. Virol.,
July 1, 2004;
78(13):
7257 - 7263.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Zucca, A. Conconi, E. Pedrinis, S. Cortelazzo, T. Motta, M. K. Gospodarowicz, B. J. Patterson, A. J. M. Ferreri, M. Ponzoni, L. Devizzi, et al.
Nongastric marginal zone B-cell lymphoma of mucosa-associated lymphoid tissue
Blood,
April 1, 2003;
101(7):
2489 - 2495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Hermine, F. Lefrere, J.-P. Bronowicki, X. Mariette, K. Jondeau, V. Eclache-Saudreau, B. Delmas, F. Valensi, P. Cacoub, C. Brechot, et al.
Regression of Splenic Lymphoma with Villous Lymphocytes after Treatment of Hepatitis C Virus Infection
N. Engl. J. Med.,
July 11, 2002;
347(2):
89 - 94.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Algara, M. S. Mateo, M. Sanchez-Beato, M. Mollejo, I. C. Navas, L. Romero, F. Sole, M. Salido, L. Florensa, P. Martinez, et al.
Analysis of the IgVH somatic mutations in splenic marginal zone lymphoma defines a group of unmutated cases with frequent 7q deletion and adverse clinical course
Blood,
February 15, 2002;
99(4):
1299 - 1304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. R. Quinn, C. H. Chan, K. G. Hadlock, S. K. H. Foung, M. Flint, and S. Levy
The B-cell receptor of a hepatitis C virus (HCV)-associated non-Hodgkin lymphoma binds the viral E2 envelope protein, implicating HCV in lymphomagenesis
Blood,
December 15, 2001;
98(13):
3745 - 3749.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Marasca, P. Vaccari, M. Luppi, P. Zucchini, I. Castelli, P. Barozzi, A. Cuoghi, and G. Torelli
Immunoglobulin Gene Mutations and Frequent Use of VH1-69 and VH4-34 Segments in Hepatitis C Virus-Positive and Hepatitis C Virus-Negative Nodal Marginal Zone B-Cell Lymphoma
Am. J. Pathol.,
July 1, 2001;
159(1):
253 - 261.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Knight, V. Agnello, M. Boiocchi, V. De Re, D. Gasparotto, and S. De Vita
WA monoclonal rheumatoid factors and non-Hodgkin lymphoma
Blood,
May 15, 2001;
97(10):
3319 - 3321.
[Full Text]
[PDF]
|
|
|
| |
