SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Salzer, U. Articles by Prohaska, R. Search for Related Content PubMed PubMed Citation Articles by Salzer, U. Articles by Prohaska, R. Related Collections Red Cells Signal Transduction Brief Reports Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 February 2001, Vol. 97, No. 4, pp. 1141-1143 BRIEF REPORT Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts Ulrich Salzer and Rainer Prohaska From the Institute of Medical Biochemistry, University of Vienna, Vienna Biocenter, Vienna, Austria. Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents, have a low buoyant density, and preferentially contain lipid-modified proteins, like glycosyl phosphatidylinositol (GPI)-anchored proteins. The lipid rafts were isolated from human erythrocytes and major protein components were identified. Apart from the GPI-anchored proteins, the most abundant integral proteins were found to be the distantly related membrane proteins stomatin (band 7.2b), flotillin-1, and flotillin-2. Flotillins, already described as lipid raft components in neurons and caveolae-associated proteins in A498 kidney cells, have not been recognized as red cell components yet. In addition, it was shown that the major cytoskeletal proteins, spectrin, actin, band 4.1, and band 4.2, are partly associated with the lipid rafts. Stomatin and the flotillins are present as independently organized high-order oligomers, suggesting that these complexes act as separate scaffolding components at the cytoplasmic face of erythrocyte lipid rafts. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Donovan and M. Bramkamp Characterization and subcellular localization of a bacterial flotillin homologue Microbiology, June 1, 2009; 155(6): 1786 - 1799. [Abstract] [Full Text] [PDF] C. Morenilla-Palao, M. Pertusa, V. Meseguer, H. Cabedo, and F. Viana Lipid Raft Segregation Modulates TRPM8 Channel Activity J. Biol. Chem., April 3, 2009; 284(14): 9215 - 9224. [Abstract] [Full Text] [PDF] A. Minami, M. Fujiwara, A. Furuto, Y. Fukao, T. Yamashita, M. Kamo, Y. Kawamura, and M. Uemura Alterations in Detergent-Resistant Plasma Membrane Microdomains in Arabidopsis thaliana During Cold Acclimation Plant Cell Physiol., February 1, 2009; 50(2): 341 - 359. [Abstract] [Full Text] [PDF] N. Mohandas and P. G. Gallagher Red cell membrane: past, present, and future Blood, November 15, 2008; 112(10): 3939 - 3948. [Abstract] [Full Text] [PDF] M. G. Kirchhof, L. A. Chau, C. D. Lemke, S. Vardhana, P. J. Darlington, M. E. Marquez, R. Taylor, K. Rizkalla, I. Blanca, M. L. Dustin, et al. Modulation of T Cell Activation by Stomatin-Like Protein 2 J. Immunol., August 1, 2008; 181(3): 1927 - 1936. [Abstract] [Full Text] [PDF] S. C. Murphy, S. Fernandez-Pol, P. H. Chung, S. N. Prasanna Murthy, S. B. Milne, M. Salomao, H. A. Brown, J. W. Lomasney, N. Mohandas, and K. Haldar Cytoplasmic remodeling of erythrocyte raft lipids during infection by the human malaria parasite Plasmodium falciparum Blood, September 15, 2007; 110(6): 2132 - 2139. [Abstract] [Full Text] [PDF] T. Jahn, E. Leifheit, S. Gooch, S. Sindhu, and K. Weinberg Lipid rafts are required for Kit survival and proliferation signals Blood, September 15, 2007; 110(6): 1739 - 1747. [Abstract] [Full Text] [PDF] F. A. Kuypers Membrane Lipid Alterations in Hemoglobinopathies Hematology, January 1, 2007; 2007(1): 68 - 73. [Abstract] [Full Text] [PDF] J. P. Laliberte, L. W. McGinnes, M. E. Peeples, and T. G. Morrison Integrity of membrane lipid rafts is necessary for the ordered assembly and release of infectious newcastle disease virus particles. J. Virol., November 1, 2006; 80(21): 10652 - 10662. [Abstract] [Full Text] [PDF] E. Umlauf, M. Mairhofer, and R. Prohaska Characterization of the Stomatin Domain Involved in Homo-oligomerization and Lipid Raft Association J. Biol. Chem., August 18, 2006; 281(33): 23349 - 23356. [Abstract] [Full Text] [PDF] D. T. Browman, M. E. Resek, L. D. Zajchowski, and S. M. Robbins Erlin-1 and erlin-2 are novel members of the prohibitin family of proteins that define lipid-raft-like domains of the ER J. Cell Sci., August 1, 2006; 119(15): 3149 - 3160. [Abstract] [Full Text] [PDF] S. Frankland, A. Adisa, P. Horrocks, T. F. Taraschi, T. Schneider, S. R. Elliott, S. J. Rogerson, E. Knuepfer, A. F. Cowman, C. I. Newbold, et al. Delivery of the Malaria Virulence Protein PfEMP1 to the Erythrocyte Surface Requires Cholesterol-Rich Domains Eukaryot. Cell, May 1, 2006; 5(5): 849 - 860. [Abstract] [Full Text] [PDF] A. Nonami, T. Taketomi, A. Kimura, K. Saeki, H. Takaki, T. Sanada, K. Taniguchi, M. Harada, R. Kato, and A. Yoshimura The Sprouty-related protein, Spred-1, localizes in a lipid raft/caveola and inhibits ERK activation in collaboration with caveolin-1 Genes Cells, September 1, 2005; 10(9): 887 - 895. [Abstract] [Full Text] [PDF] D. R. Drew, P. R. Sanders, and B. S. Crabb Plasmodium falciparum Merozoite Surface Protein 8 Is a Ring-Stage Membrane Protein That Localizes to the Parasitophorous Vacuole of Infected Erythrocytes Infect. Immun., July 1, 2005; 73(7): 3912 - 3922. [Abstract] [Full Text] [PDF] R. D. Bagshaw, D. J. Mahuran, and J. W. Callahan A Proteomic Analysis of Lysosomal Integral Membrane Proteins Reveals the Diverse Composition of the Organelle Mol. Cell. Proteomics, February 1, 2005; 4(2): 133 - 143. [Abstract] [Full Text] [PDF] M. P. Price, R. J. Thompson, J. O. Eshcol, J. A. Wemmie, and C. J. Benson Stomatin Modulates Gating of Acid-sensing Ion Channels J. Biol. Chem., December 17, 2004; 279(51): 53886 - 53891. [Abstract] [Full Text] [PDF] M. Inoue, M. A. Digman, M. Cheng, S. Y. Breusegem, N. Halaihel, V. Sorribas, W. W. Mantulin, E. Gratton, N. P. Barry, and M. Levi Partitioning of NaPi Cotransporter in Cholesterol-, Sphingomyelin-, and Glycosphingolipid-enriched Membrane Domains Modulates NaPi Protein Diffusion, Clustering, and Activity J. Biol. Chem., November 19, 2004; 279(47): 49160 - 49171. [Abstract] [Full Text] [PDF] A. W. Cohen, R. Hnasko, W. Schubert, and M. P. Lisanti Role of Caveolae and Caveolins in Health and Disease Physiol Rev, October 1, 2004; 84(4): 1341 - 1379. [Abstract] [Full Text] [PDF] M. W. Musch, D.-L. T. Koomoa, and L. Goldstein Hypotonicity-induced Exocytosis of the Skate Anion Exchanger skAE1: ROLE OF LIPID RAFT REGIONS J. Biol. Chem., September 17, 2004; 279(38): 39447 - 39453. [Abstract] [Full Text] [PDF] E. Umlauf, E. Csaszar, M. Moertelmaier, G. J. Schuetz, R. G. Parton, and R. Prohaska Association of Stomatin with Lipid Bodies J. Biol. Chem., May 28, 2004; 279(22): 23699 - 23709. [Abstract] [Full Text] [PDF] K. Haglund, I. Ivankovic-Dikic, N. Shimokawa, G. D. Kruh, and I. Dikic Recruitment of Pyk2 and Cbl to lipid rafts mediates signals important for actin reorganization in growing neurites J. Cell Sci., May 15, 2004; 117(12): 2557 - 2568. [Abstract] [Full Text] [PDF] D. G. Kakhniashvili, L. A. Bulla Jr., and S. R. Goodman The Human Erythrocyte Proteome: Analysis by Ion Trap Mass Spectrometry Mol. Cell. Proteomics, May 1, 2004; 3(5): 501 - 509. [Abstract] [Full Text] [PDF] S. C. Murphy, B. U. Samuel, T. Harrison, K. D. Speicher, D. W. Speicher, M. E. Reid, R. Prohaska, P. S. Low, M. J. Tanner, N. Mohandas, et al. Erythrocyte detergent-resistant membrane proteins: their characterization and selective uptake during malarial infection Blood, March 1, 2004; 103(5): 1920 - 1928. [Abstract] [Full Text] [PDF] M. Ohara-Imaizumi, C. Nishiwaki, T. Kikuta, K. Kumakura, Y. Nakamichi, and S. Nagamatsu Site of Docking and Fusion of Insulin Secretory Granules in Live MIN6 {beta} Cells Analyzed by TAT-conjugated Anti-syntaxin 1 Antibody and Total Internal Reflection Fluorescence Microscopy J. Biol. Chem., February 27, 2004; 279(9): 8403 - 8408. [Abstract] [Full Text] [PDF] A. de Gassart, C. Geminard, B. Fevrier, G. Raposo, and M. Vidal Lipid raft-associated protein sorting in exosomes Blood, December 15, 2003; 102(13): 4336 - 4344. [Abstract] [Full Text] [PDF] N. L. Hiller, T. Akompong, J. S. Morrow, A. A. Holder, and K. Haldar Identification of a Stomatin Orthologue in Vacuoles Induced in Human Erythrocytes by Malaria Parasites: A ROLE FOR MICROBIAL RAFT PROTEINS IN APICOMPLEXAN VACUOLE BIOGENESIS J. Biol. Chem., November 28, 2003; 278(48): 48413 - 48421. [Abstract] [Full Text] [PDF] M. L. Hixon and K. Boekelheide Expression and Localization of Total Akt1 and Phosphorylated Akt1 in the Rat Seminiferous Epithelium J Androl, November 1, 2003; 24(6): 891 - 898. [Abstract] [Full Text] [PDF] B. Fricke, A. C. Argent, M. C. Chetty, A. R. Pizzey, E. J. Turner, M. M. Ho, A. Iolascon, M. von During, and G. W. Stewart The "stomatin" gene and protein in overhydrated hereditary stomatocytosis Blood, September 15, 2003; 102(6): 2268 - 2277. [Abstract] [Full Text] [PDF] D. Rubin and F. Ismail-Beigi Distribution of Glut1 in detergent-resistant membranes (DRMs) and non-DRM domains: effect of treatment with azide Am J Physiol Cell Physiol, August 1, 2003; 285(2): C377 - C383. [Abstract] [Full Text] [PDF] I. Leshchyns'ka, V. Sytnyk, J. S. Morrow, and M. Schachner Neural cell adhesion molecule (NCAM) association with PKC{beta}2 via {beta}I spectrin is implicated in NCAM-mediated neurite outgrowth J. Cell Biol., May 12, 2003; 161(3): 625 - 639. [Abstract] [Full Text] [PDF] G. Minetti, A. Ciana, U. Salzer, and R. Prohaska New and old integral proteins of the human erythrocyte membrane Blood, May 1, 2003; 101(9): 3751 - 3752. [Full Text] [PDF] K. Strange From Genes to Integrative Physiology: Ion Channel and Transporter Biology in Caenorhabditis elegans Physiol Rev, April 1, 2003; 83(2): 377 - 415. [Abstract] [Full Text] [PDF] E. Feuk-Lagerstedt, M. Samuelsson, W. Mosgoeller, C. Movitz, A. Rosqvist, J. Bergstrom, T. Larsson, M. Steiner, R. Prohaska, and A. Karlsson The presence of stomatin in detergent-insoluble domains of neutrophil granule membranes J. Leukoc. Biol., November 1, 2002; 72(5): 970 - 977. [Abstract] [Full Text] [PDF] T. Nebl, K. N. Pestonjamasp, J. D. Leszyk, J. L. Crowley, S. W. Oh, and E. J. Luna Proteomic Analysis of a Detergent-resistant Membrane Skeleton from Neutrophil Plasma Membranes J. Biol. Chem., November 1, 2002; 277(45): 43399 - 43409. [Abstract] [Full Text] [PDF] M. A. Saleem, L. Ni, I. Witherden, K. Tryggvason, V. Ruotsalainen, P. Mundel, and P. W. Mathieson Co-Localization of Nephrin, Podocin, and the Actin Cytoskeleton : Evidence for a Role in Podocyte Foot Process Formation Am. J. Pathol., October 1, 2002; 161(4): 1459 - 1466. [Abstract] [Full Text] [PDF] M. Mairhofer, M. Steiner, W. Mosgoeller, R. Prohaska, and U. Salzer Stomatin is a major lipid-raft component of platelet alpha granules Blood, July 18, 2002; 100(3): 897 - 904. [Abstract] [Full Text] [PDF] K. Kobayakawa, R. Hayashi, K. Morita, K. Miyamichi, Y. Oka, A. Tsuboi, and H. Sakano Stomatin-Related Olfactory Protein, SRO, Specifically Expressed in the Murine Olfactory Sensory Neurons J. Neurosci., July 15, 2002; 22(14): 5931 - 5937. [Abstract] [Full Text] [PDF] M. Hekman, H. Hamm, A. V. Villar, B. Bader, J. Kuhlmann, J. Nickel, and U. R. Rapp Associations of B- and C-Raf with Cholesterol, Phosphatidylserine, and Lipid Second Messengers. PREFERENTIAL BINDING OF Raf TO ARTIFICIAL LIPID RAFTS J. Biol. Chem., June 28, 2002; 277(27): 24090 - 24102. [Abstract] [Full Text] [PDF] U. Salzer, P. Hinterdorfer, U. Hunger, C. Borken, and R. Prohaska Ca++-dependent vesicle release from erythrocytes involves stomatin-specific lipid rafts, synexin (annexin VII), and sorcin Blood, April 1, 2002; 99(7): 2569 - 2577. [Abstract] [Full Text] [PDF] C. A.O. Stuermer, D. M. Lang, F. Kirsch, M. Wiechers, S.-O. Deininger, and H. Plattner Glycosylphosphatidyl Inositol-anchored Proteins and fyn Kinase Assemble in Noncaveolar Plasma Membrane Microdomains Defined by Reggie-1 and -2 Mol. Biol. Cell, October 1, 2001; 12(10): 3031 - 3045. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020