|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 February 2001, Vol. 97, No. 4, pp. 922-928
HEMATOPOIESIS
Overlapping roles for granulocyte-macrophage colony-stimulating
factor and interleukin-3 in eosinophil homeostasis and contact
hypersensitivity
Silke Gillessen,
Nicolas Mach,
Clayton Small,
Martin Mihm, and
Glenn Dranoff
From the Department of Adult Oncology, Dana-Farber
Cancer Institute and Department of Medicine, Harvard Medical School,
Boston, MA; Division of Oncology, Department of Internal Medicine,
Geneva University Hospital, Geneva, Switzerland; and Department of
Pathology, Massachusetts General Hospital, Boston, MA.
Studies of mice rendered deficient in granulocyte-macrophage
colony-stimulating factor (GM-CSF) or interleukin-3 (IL-3) have established unique roles for these cytokines in pulmonary homeostasis, resistance to infection, and antigen-specific T- and B-cell responses. In addition to these distinctive properties, however, GM-CSF and IL-3
also stimulate the development and activation of hematopoietic cells in
many similar ways, raising the possibility that each factor might
partially compensate for the other's absence in singly deficient mice.
To test whether endogenous GM-CSF and IL-3 mediate redundant functions
in vivo, we generated mice lacking both cytokines through sequential
gene targeting experiments in embryonic stem (ES) cells. Surprisingly,
doubly deficient animals, but not single knockouts, showed increased
numbers of circulating eosinophils. Doubly deficient mice, moreover,
developed weaker contact hypersensitivity reactions to haptens applied
epicutaneously than mice deficient in either factor alone. Together,
these findings delineate overlapping roles for GM-CSF and IL-3 in
hematopoiesis and immunity.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
S. Zarei, F. Schwenter, P. Luy, M. Aurrand-Lions, P. Morel, M. Kopf, G. Dranoff, and N. Mach
Role of GM-CSF signaling in cell-based tumor immunization
Blood,
June 25, 2009;
113(26):
6658 - 6668.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Dyer, J. M. Moser, M. Czapiga, S. J. Siegel, C. M. Percopo, and H. F. Rosenberg
Functionally Competent Eosinophils Differentiated Ex Vivo in High Purity from Normal Mouse Bone Marrow
J. Immunol.,
September 15, 2008;
181(6):
4004 - 4009.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Alisa, S. Boswell, A. A. Pathan, L. Ayaru, R. Williams, and S. Behboudi
Human CD4+ T Cells Recognize an Epitope within {alpha}-Fetoprotein Sequence and Develop into TGF-{beta}-Producing CD4+ T Cells
J. Immunol.,
April 1, 2008;
180(7):
5109 - 5117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Enzler, S. Gillessen, M. Dougan, J. P. Allison, D. Neuberg, D. A. Oble, M. Mihm, and G. Dranoff
Functional deficiencies of granulocyte-macrophage colony stimulating factor and interleukin-3 contribute to insulitis and destruction of {beta} cells
Blood,
August 1, 2007;
110(3):
954 - 961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. B. Aiello, J. R. Keller, K. D. Klarmann, G. Dranoff, R. Mazzucchelli, and S. K. Durum
IL-7 Induces Myelopoiesis and Erythropoiesis
J. Immunol.,
February 1, 2007;
178(3):
1553 - 1563.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Iwasaki, C. Somoza, H. Shigematsu, E. A. Duprez, J. Iwasaki-Arai, S.-i. Mizuno, Y. Arinobu, K. Geary, P. Zhang, T. Dayaram, et al.
Distinctive and indispensable roles of PU.1 in maintenance of hematopoietic stem cells and their differentiation
Blood,
September 1, 2005;
106(5):
1590 - 1600.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Terszowski, C. Waskow, P. Conradt, D. Lenze, J. Koenigsmann, D. Carstanjen, I. Horak, and H.-R. Rodewald
Prospective isolation and global gene expression analysis of the erythrocyte colony-forming unit (CFU-E)
Blood,
March 1, 2005;
105(5):
1937 - 1945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Sitnicka, C. Brakebusch, I.-L. Martensson, M. Svensson, W. W. Agace, M. Sigvardsson, N. Buza-Vidas, D. Bryder, C. M.Cilio, H. Ahlenius, et al.
Complementary Signaling through flt3 and Interleukin-7 Receptor {alpha} Is Indispensable for Fetal and Adult B Cell Genesis
J. Exp. Med.,
November 17, 2003;
198(10):
1495 - 1506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. G. Ribeiro, D. A. Maria, S. Adriouch, S. Pechberty, W. H. K. Cabrera, J. Morisset, O. M. Ibanez, and M. Seman
Convergent alteration of granulopoiesis, chemotactic activity, and neutrophil apoptosis during mouse selection for high acute inflammatory response
J. Leukoc. Biol.,
October 1, 2003;
74(4):
497 - 506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Iwasaki-Arai, H. Iwasaki, T. Miyamoto, S. Watanabe, and K. Akashi
Enforced Granulocyte/Macrophage Colony-stimulating Factor Signals Do Not Support Lymphopoiesis, but Instruct Lymphoid to Myelomonocytic Lineage Conversion
J. Exp. Med.,
May 19, 2003;
197(10):
1311 - 1322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Enzler, S. Gillessen, J. P. Manis, D. Ferguson, J. Fleming, F. W. Alt, M. Mihm, and G. Dranoff
Deficiencies of GM-CSF and Interferon {gamma} Link Inflammation and Cancer
J. Exp. Med.,
May 5, 2003;
197(9):
1213 - 1219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Jegalian, A. Acurio, G. Dranoff, and H. Wu
Erythropoietin receptor haploinsufficiency and in vivo interplay with granulocyte-macrophage colony-stimulating factor and interleukin 3
Blood,
April 1, 2002;
99(7):
2603 - 2605.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
