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Related Collections Hematopoiesis and Stem Cells Hemostasis, Thrombosis, and Vascular Biology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 March 2001, Vol. 97, No. 5, pp. 1282-1288 HEMATOPOIESIS Synergistic mobilization of hemopoietic progenitor cells using concurrent 1 and 2 integrin blockade or 2-deficient mice Thalia Papayannopoulou, Gregory V. Priestley, Betty Nakamoto, Vivian Zafiropoulos, Linda M. Scott, and John M. Harlan From the University of Washington, Seattle, WA. The hierarchy of cytoadhesion molecules involved in hematopoietic/stem progenitor cell mobilization has not yet been delineated. Previous studies have suggested an important role for 41 integrin in this process. To test whether mobilization involves dynamic interactions of 41 with other integrins on hematopoietic cells, especially the 2 integrins, mice and primates were treated with anti-1 or anti-2 antibodies alone or in combination. A single injection of anti-41 antibody elicited reproducible mobilization in contrast to other antibodies, and 3 injections yielded higher mobilization efficiency than each of the other antibodies. When the anti-2 (anti-CD11a or anti-CD18) or anti-5/1 integrin antibody was combined with anti-4, an augmentation in mobilization was seen that was either additive or synergistic, depending on the potency of the antibody used. Synergy between anti-4 and anti-CD18 (2) antibody blockade was seen in primates and confirmed in anti-4-treated CD18-deficient mice. In the latter, there was a 49-fold increase in mobilization with anti-4, much higher than in littermate control animals, in CD18 hypomorphic mice, or in other strains of mice tested. Data from both the antibody blockade and gene-targeted mice suggest that the cooperativity of 41 with 2 integrins becomes evident when they are concurrently inhibited. It is unclear whether this cooperativity is exerted at the stage of reversible adhesion versus migration, and enhancement of and whether the 2 integrins work in a sequential or parallel manner. Whatever the mechanism, the data provide a novel example of 1 and 2 integrin crosstalk in stem/progenitor cell mobilization. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. J. Christopher, F. Liu, M. J. Hilton, F. Long, and D. C. Link Suppression of CXCL12 production by bone marrow osteoblasts is a common and critical pathway for cytokine-induced mobilization Blood, August 13, 2009; 114(7): 1331 - 1339. [Abstract] [Full Text] [PDF] J. Foguenne, I. Di Stefano, O. Giet, Y. Beguin, and A. 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