SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Krishnaraju, K. Articles by Liebermann, D. A. Search for Related Content PubMed PubMed Citation Articles by Krishnaraju, K. Articles by Liebermann, D. A. Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 March 2001, Vol. 97, No. 5, pp. 1298-1305 HEMATOPOIESIS Early growth response gene 1 stimulates development of hematopoietic progenitor cells along the macrophage lineage at the expense of the granulocyte and erythroid lineages Kandasamy Krishnaraju, Barbara Hoffman, and Dan A. Liebermann From the Fels Institute for Cancer Research and Molecular Biology and the Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA. Using a variety of differentiation-inducible myeloid cell lines, we previously showed that the zinc-finger transcription factor early growth response gene 1 (Egr-1) is a positive modulator of macrophage differentiation and negatively regulates granulocytic differentiation. In this study, high-efficiency retroviral transduction was used to ectopically express Egr-1 in myeloid-enriched or stem cell-enriched bone marrow cultures to explore its effect on the development of hematopoietic progenitors in vitro and in lethally irradiated mice. It was found that ectopic Egr-1 expression in normal hematopoietic progenitors stimulates development along the macrophage lineage at the expense of development along the granulocyte or erythroid lineages, regardless of the cytokine used. Moreover, Egr-1 accelerated macrophage development by suppressing the proliferative phase of the growth-to-macrophage developmental program. The remarkable ability of Egr-1 to dictate macrophage development at the expense of development along other lineages resulted in failure of Egr-1-infected hematopoietic progenitors to repopulate the bone marrow and spleen, and thereby prevent death, in lethally irradiated mice. These observations further highlight the role Egr-1 plays in monocytic differentiation and growth suppression. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. M. Lubieniecka, D. R.H. de Bruijn, L. Su, A. H.A. van Dijk, S. Subramanian, M. van de Rijn, N. Poulin, A. G. van Kessel, and T. O. Nielsen Histone Deacetylase Inhibitors Reverse SS18-SSX-Mediated Polycomb Silencing of the Tumor Suppressor Early Growth Response 1 in Synovial Sarcoma Cancer Res., June 1, 2008; 68(11): 4303 - 4310. [Abstract] [Full Text] [PDF] B. Li, J. Berman, J.-T. Tang, and T.-J. Lin The Early Growth Response Factor-1 Is Involved in Stem Cell Factor (SCF)-induced Interleukin 13 Production by Mast Cells, but Is Dispensable for SCF-dependent Mast Cell Growth J. Biol. Chem., August 3, 2007; 282(31): 22573 - 22581. [Abstract] [Full Text] [PDF] J. H. Carter and W. G. Tourtellotte Early Growth Response Transcriptional Regulators Are Dispensable for Macrophage Differentiation J. Immunol., March 1, 2007; 178(5): 3038 - 3047. [Abstract] [Full Text] [PDF] A. Gehrig, T. Langmann, F. Horling, A. Janssen, M. Bonin, M. Walter, S. Poths, and B. H. F. Weber Genome-Wide Expression Profiling of the Retinoschisin-Deficient Retina in Early Postnatal Mouse Development Invest. Ophthalmol. Vis. Sci., February 1, 2007; 48(2): 891 - 900. [Abstract] [Full Text] [PDF] W. Yuan, J. E. Payton, M. S. Holt, D. C. Link, M. A. Watson, J. F. DiPersio, and T. J. Ley Commonly dysregulated genes in murine APL cells Blood, February 1, 2007; 109(3): 961 - 970. [Abstract] [Full Text] [PDF] B. Li, M. R. Power, and T.-J. Lin De novo synthesis of early growth response factor-1 is required for the full responsiveness of mast cells to produce TNF and IL-13 by IgE and antigen stimulation Blood, April 1, 2006; 107(7): 2814 - 2820. [Abstract] [Full Text] [PDF] J. Dauffy, G. Mouchiroud, and R. P. Bourette The interferon-inducible gene, Ifi204, is transcriptionally activated in response to M-CSF, and its expression favors macrophage differentiation in myeloid progenitor cells J. Leukoc. Biol., January 1, 2006; 79(1): 173 - 183. [Abstract] [Full Text] [PDF] W. Wagner, R. Saffrich, U. Wirkner, V. Eckstein, J. Blake, A. Ansorge, C. Schwager, F. Wein, K. Miesala, W. Ansorge, et al. Hematopoietic Progenitor Cells and Cellular Microenvironment: Behavioral and Molecular Changes upon Interaction Stem Cells, September 1, 2005; 23(8): 1180 - 1191. [Abstract] [Full Text] [PDF] M. Shafarenko, D. A. Liebermann, and B. Hoffman Egr-1 abrogates the block imparted by c-Myc on terminal M1 myeloid differentiation Blood, August 1, 2005; 106(3): 871 - 878. [Abstract] [Full Text] [PDF] R. Manfredini, R. Zini, S. Salati, M. Siena, E. Tenedini, E. Tagliafico, M. Montanari, T. Zanocco-Marani, C. Gemelli, T. Vignudelli, et al. The Kinetic Status of Hematopoietic Stem Cell Subpopulations Underlies a Differential Expression of Genes Involved in Self-Renewal, Commitment, and Engraftment Stem Cells, April 1, 2005; 23(4): 496 - 506. [Abstract] [Full Text] [PDF] F. Chen, Q. Wang, X. Wang, and G. P. Studzinski Up-Regulation of Egr1 by 1,25-Dihydroxyvitamin D3 Contributes to Increased Expression of p35 Activator of Cyclin-Dependent Kinase 5 and Consequent Onset of the Terminal Phase of HL60 Cell Differentiation Cancer Res., August 1, 2004; 64(15): 5425 - 5433. [Abstract] [Full Text] [PDF] H. Liu, J. R. Keefer, Q.-f. Wang, and A. D. Friedman Reciprocal effects of C/EBPalpha and PKCdelta on JunB expression and monocytic differentiation depend upon the C/EBPalpha basic region Blood, May 15, 2003; 101(10): 3885 - 3892. [Abstract] [Full Text] [PDF] R. M. Tjin Tham Sjin, K. Krishnaraju, B. Hoffman, and D. A. Liebermann Transcriptional regulation of myeloid differentiation primary response (MyD) genes during myeloid differentiation is mediated by nuclear factor Y Blood, June 17, 2002; 100(1): 80 - 88. [Abstract] [Full Text] [PDF] C. Amendt, A. Mann, P. Schirmacher, and M. Blessing Resistance of keratinocytes to TGF{beta}-mediated growth restriction and apoptosis induction accelerates re-epithelialization in skin wounds J. Cell Sci., May 15, 2002; 115(10): 2189 - 2198. [Abstract] [Full Text] [PDF] R. J. Steptoe, J. M. Ritchie, and L. C. Harrison Increased Generation of Dendritic Cells from Myeloid Progenitors in Autoimmune-Prone Nonobese Diabetic Mice J. Immunol., May 15, 2002; 168(10): 5032 - 5041. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020