|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 March 2001, Vol. 97, No. 5, pp. 1511-1513
BRIEF REPORT
The diversity of rearranged immunoglobulin heavy chain variable
region genes in peripheral blood B cells of preterm infants is
restricted by short third complementarity-determining regions but not
by limited gene segment usage
Michael Zemlin,
Karl Bauer,
Michael Hummel,
Sabine Pfeiffer,
Simone Devers,
Cosima Zemlin,
Harald Stein, and
Hans T. Versmold
From the Department of Pediatrics and Pathology, Freie
Universität Berlin, Berlin, Germany.
The immunoglobulin diversity is restricted in fetal liver B cells.
This study examined whether peripheral blood B cells of extremely
preterm infants show similar restrictions (overrepresentation of some
gene segments, short third complementarity-determining regions
[CDR3]). DNA of rearranged immunoglobulin heavy chain genes was
amplified by polymerase chain reaction, cloned, and sequenced. A total
of 417 sequences were analyzed from 6 preterm infants (25-28 weeks of
gestation), 6 term infants, and 6 adults. Gene segments from the
entire VH and DH gene
locus were rearranged in preterm infants, even though the
DH7-27 segment was overrepresented (17% of rearrangements)
compared to term infants (7%) and adults (2%). CDR3 was shorter in
preterm infants (40 ± 10 nucleotides) than in term infants
(44 ± 12) and adults (48 ± 14) (P < .001) due to
shorter N regions. Somatic mutations were exclusively found in term
neonates and adults (mutational frequency 0.8% and 1.8%). We conclude
that preterm infants have no limitations in gene segment usage, whereas
the diversity of CDR3 is restricted throughout gestation.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Zemlin, G. Hoersch, C. Zemlin, A. Pohl-Schickinger, M. Hummel, C. Berek, R. F. Maier, and K. Bauer
The Postnatal Maturation of the Immunoglobulin Heavy Chain IgG Repertoire in Human Preterm Neonates Is Slower than in Term Neonates
J. Immunol.,
January 15, 2007;
178(2):
1180 - 1188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Souto-Carneiro, G. P. Sims, H. Girschik, J. Lee, and P. E. Lipsky
Developmental Changes in the Human Heavy Chain CDR3
J. Immunol.,
December 1, 2005;
175(11):
7425 - 7436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Stamatopoulos, C. Belessi, A. Hadzidimitriou, T. Smilevska, E. Kalagiakou, K. Hatzi, N. Stavroyianni, A. Athanasiadou, A. Tsompanakou, T. Papadaki, et al.
Immunoglobulin light chain repertoire in chronic lymphocytic leukemia
Blood,
November 15, 2005;
106(10):
3575 - 3583.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. P. Sims, R. Ettinger, Y. Shirota, C. H. Yarboro, G. G. Illei, and P. E. Lipsky
Identification and characterization of circulating human transitional B cells
Blood,
June 1, 2005;
105(11):
4390 - 4398.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Jackson and J. D. Capra
IgH V-Region Sequence Does Not Predict the Survival Fate of Human Germinal Center B Cells
J. Immunol.,
March 1, 2005;
174(5):
2805 - 2813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kriangkum, B. J. Taylor, S. P. Treon, M. J. Mant, A. R. Belch, and L. M. Pilarski
Clonotypic IgM V/D/J sequence analysis in Waldenstrom macroglobulinemia suggests an unusual B-cell origin and an expansion of polyclonal B cells in peripheral blood
Blood,
October 1, 2004;
104(7):
2134 - 2142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Li, M. Rue, J. Zhou, H. Wang, M. A. Goldwasser, D. Neuberg, V. Dalton, D. Zuckerman, C. Lyons, L. B. Silverman, et al.
Utilization of Ig heavy chain variable, diversity, and joining gene segments in children with B-lineage acute lymphoblastic leukemia: implications for the mechanisms of VDJ recombination and for pathogenesis
Blood,
June 15, 2004;
103(12):
4602 - 4609.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-H. Weitkamp, N. Kallewaard, K. Kusuhara, E. Bures, J. V. Williams, B. LaFleur, H. B. Greenberg, and J. E. Crowe Jr.
Infant and Adult Human B Cell Responses to Rotavirus Share Common Immunodominant Variable Gene Repertoires
J. Immunol.,
November 1, 2003;
171(9):
4680 - 4688.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. L. Wiemels, B. C. Leonard, Y. Wang, M. R. Segal, S. P. Hunger, M. T. Smith, V. Crouse, X. Ma, P. A. Buffler, and S. R. Pine
Site-specific translocation and evidence of postnatal origin of the t(1;19) E2A-PBX1 fusion in childhood acute lymphoblastic leukemia
PNAS,
November 12, 2002;
99(23):
15101 - 15106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Bauer, M. Zemlin, M. Hummel, S. Pfeiffer, J. Karstaedt, G. Steinhauser, X. Xiao, H. Versmold, and C. Berek
Diversification of Ig Heavy Chain Genes in Human Preterm Neonates Prematurely Exposed to Environmental Antigens
J. Immunol.,
August 1, 2002;
169(3):
1349 - 1356.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. W. Schroeder Jr, L. Zhang, and J. B. Philips III
Slow, programmed maturation of the immunoglobulin HCDR3 repertoire during the third trimester of fetal life
Blood,
November 1, 2001;
98(9):
2745 - 2751.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. D. Rossi, K. L. Medina, K. Garrett, G. Kolar, P. C. Comp, L. D. Shultz, J. D. Capra, P. Wilson, A. Schipul, and P. W. Kincade
Relatively Normal Human Lymphopoiesis but Rapid Turnover of Newly Formed B Cells in Transplanted Nonobese Diabetic/SCID Mice
J. Immunol.,
September 15, 2001;
167(6):
3033 - 3042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
