|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 March 2001, Vol. 97, No. 6, pp. 1703-1711
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Urokinase-dependent plasminogen activation is required for
efficient skeletal muscle regeneration in vivo
Frederic Lluís,
Josep Roma,
Mònica Suelves,
Maribel Parra,
Gloria Aniorte,
Eduard Gallardo,
Isabel Illa,
Luciano Rodríguez,
Simon M. Hughes,
Peter Carmeliet,
Manuel Roig, and
Pura Muñoz-Cánoves
From the Centre d'Oncologia Molecular, Institut de
Recerca Oncològica; the Unitat de Recerca de la Vall d'Hebron;
the Hospital de la Santa Creu i Sant Pau, Barcelona, Spain; the Center
for Transgene Technology, Flanders Interuniversity, Leuven, Belgium;
and the Randall Centre, New Hunt's House, Kings College, London,
United Kingdom.
Plasminogen activators urokinase-type plasminogen activator (uPA)
and tissue-type plasminogen activator (tPA) are extracellular proteases
involved in various tissue remodeling processes. A requirement for uPA
activity in skeletal myogenesis was recently demonstrated in vitro. The
role of plasminogen activators in skeletal muscle regeneration in vivo
in wild-type, uPA-deficient, and tPA-deficient mice is investigated
here. Wild-type and tPA / mice completely repaired experimentally
damaged skeletal muscle. In contrast, uPA/ mice had a severe
regeneration defect, with decreased recruitment of blood-derived
monocytes to the site of injury and with persistent myotube
degeneration. In addition, uPA-deficient mice accumulated fibrin in the
degenerating muscle fibers; however, the defibrinogenation of
uPA-deficient mice resulted in a correction of the muscle regeneration defect. A similar severe regeneration deficit with persistent fibrin
deposition was also reproducible in plasminogen-deficient mice after
injury, suggesting that fibrinolysis by uPA-mediated plasminogen
activation plays a fundamental role in skeletal muscle regeneration. In
conclusion, the uPA-plasmin system is identified as a critical
component of the mammalian skeletal muscle regeneration process,
possibly because it prevents intramuscular fibrin accumulation and
contributes to the adequate inflammatory response after injury. These
studies demonstrate the requirement of an extracellular proteolytic
cascade during muscle regeneration in vivo.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
J. Liu, P. B. Gurpur, and S. J. Kaufman
Genetically Determined Proteolytic Cleavage Modulates {alpha}7{beta}1 Integrin Function
J. Biol. Chem.,
December 19, 2008;
283(51):
35668 - 35678.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Vidal, A. L. Serrano, M. Tjwa, M. Suelves, E. Ardite, R. De Mori, B. Baeza-Raja, M. Martinez de Lagran, P. Lafuste, V. Ruiz-Bonilla, et al.
Fibrinogen drives dystrophic muscle fibrosis via a TGF{beta}/alternative macrophage activation pathway
Genes & Dev.,
July 1, 2008;
22(13):
1747 - 1752.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Bryer, G. Fantuzzi, N. Van Rooijen, and T. J. Koh
Urokinase-Type Plasminogen Activator Plays Essential Roles in Macrophage Chemotaxis and Skeletal Muscle Regeneration
J. Immunol.,
January 15, 2008;
180(2):
1179 - 1188.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. DiPasquale, M. Cheng, W. Billich, S. A. Huang, N. van Rooijen, T. A. Hornberger, and T. J. Koh
Urokinase-type plasminogen activator and macrophages are required for skeletal muscle hypertrophy in mice
Am J Physiol Cell Physiol,
October 1, 2007;
293(4):
C1278 - C1285.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Suelves, B. Vidal, A. L. Serrano, M. Tjwa, J. Roma, R. Lopez-Alemany, A. Luttun, M. M. de Lagran, M. A. Diaz, M. Jardi, et al.
uPA deficiency exacerbates muscular dystrophy in MDX mice
J. Cell Biol.,
September 7, 2007;
178(6):
1039 - 1051.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. C. Bryer and T. J. Koh
The urokinase-type plasminogen activator receptor is not required for skeletal muscle inflammation or regeneration
Am J Physiol Regulatory Integrative Comp Physiol,
September 1, 2007;
293(3):
R1152 - R1158.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Schachtrup, P. Lu, L. L. Jones, J. K. Lee, J. Lu, B. D. Sachs, B. Zheng, and K. Akassoglou
Fibrinogen inhibits neurite outgrowth via beta3 integrin-mediated phosphorylation of the EGF receptor
PNAS,
July 10, 2007;
104(28):
11814 - 11819.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Tidball and M. Wehling-Henricks
Macrophages promote muscle membrane repair and muscle fibre growth and regeneration during modified muscle loading in mice in vivo
J. Physiol.,
January 1, 2007;
578(1):
327 - 336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Xiang, M. D. Schuster, T. Seki, P. Witkowski, S. Eshghi, and S. Itescu
Downregulated Expression of Plasminogen Activator Inhibitor-1 Augments Myocardial Neovascularization and Reduces Cardiomyocyte Apoptosis After Acute Myocardial Infarction
J. Am. Coll. Cardiol.,
August 2, 2005;
46(3):
536 - 541.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Koh, S. C. Bryer, A. M. Pucci, and T. H. Sisson
Mice deficient in plasminogen activator inhibitor-1 have improved skeletal muscle regeneration
Am J Physiol Cell Physiol,
July 1, 2005;
289(1):
C217 - C223.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Timmons, O. Larsson, E. Jansson, H. Fischer, T. Gustafsson, P. L. Greenhaff, J. Ridden, J. Rachman, M. Peyrard-Janvid, C. Wahlestedt, et al.
Human muscle gene expression responses to endurance training provide a novel perspective on Duchenne muscular dystrophy
FASEB J,
May 1, 2005;
19(7):
750 - 760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Tidball
Inflammatory processes in muscle injury and repair
Am J Physiol Regulatory Integrative Comp Physiol,
February 1, 2005;
288(2):
R345 - R353.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Chazaud, C. Sonnet, P. Lafuste, G. Bassez, A.-C. Rimaniol, F. Poron, F.-J. Authier, P. A. Dreyfus, and R. K. Gherardi
Satellite cells attract monocytes and use macrophages as a support to escape apoptosis and enhance muscle growth
J. Cell Biol.,
December 8, 2003;
163(5):
1133 - 1143.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. E. Barani, A.-C. Durieux, O. Sabido, and D. Freyssenet
Age-related changes in the mitotic and metabolic characteristics of muscle-derived cells
J Appl Physiol,
November 1, 2003;
95(5):
2089 - 2098.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Duguez, M.-C. L. Bihan, D. Gouttefangeas, L. Feasson, and D. Freyssenet
Myogenic and nonmyogenic cells differentially express proteinases, Hsc/Hsp70, and BAG-1 during skeletal muscle regeneration
Am J Physiol Endocrinol Metab,
July 1, 2003;
285(1):
E206 - E215.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-W. Chen, G. A Nader, K. R Baar, M. J Fedele, E. P Hoffman, and K. A Esser
Response of rat muscle to acute resistance exercise defined by transcriptional and translational profiling
J. Physiol.,
November 15, 2002;
545(1):
27 - 41.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Suelves, R. Lopez-Alemany, F. Lluis, G. Aniorte, E. Serrano, M. Parra, P. Carmeliet, and P. Munoz-Canoves
Plasmin activity is required for myogenesis in vitro and skeletal muscle regeneration in vivo
Blood,
April 15, 2002;
99(8):
2835 - 2844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. D. Cook, E. L. Braine, I. K. Campbell, and J. A. Hamilton
Differing Roles for Urokinase and Tissue-Type Plasminogen Activator in Collagen-Induced Arthritis
Am. J. Pathol.,
March 1, 2002;
160(3):
917 - 926.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
