|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 March 2001, Vol. 97, No. 6, pp. 1817-1822
IMMUNOBIOLOGY
Interleukin-6 is a growth factor for nonmalignant human
plasmablasts
Gaëtan Jego,
Régis Bataille, and
Catherine Pellat-Deceunynck
From the Institut National de la Santé et de la
Recherche Médicale (INSERM) U463 and Laboratoire
d'Hématologie, Institut de Biologie, Nantes, France.
Interleukin-6 (IL-6), although often regarded as a B-cell
differentiation factor, was recently described as the essential survival factor for human plasmablasts in vivo in reactive
plasmacytosis. The present study reinvestigated the roles of IL-6 and
IL-2 in the generation of plasma cells from human memory B cells in
vitro. The cells involved in this differentiation process were
identified as preplasmablasts
(CD20±CD38±CD138 ), plasmablasts
(CD20CD38++CD138), and early
plasma cells
(CD20CD38+++CD138+++). IL-2 or
IL-10 induced a strong generation of plasmablasts and early plasma
cells (PCs). Compared to IL-2 or IL-10, IL-6 alone was inefficient at
PC generation. However, when combined with IL-2 or IL-10, IL-6 enhanced
generation of early PCs. Moreover, anti-IL-6 monoclonal antibody
markedly reduced IL-2-induced generation of early plasma cells, but
not of plasmablasts. These roles of IL-2 and IL-6 were consistent with
the difference in the expression of their respective receptors (R).
CD25 (IL-2R ) was increased 72 ± 10-fold on activated B cells, but
decreased and then disappeared on plasmablasts. Conversely, CD126
(IL-6R) was barely expressed on activated B cells, but increased
18 ± 2-fold on preplasmablasts. Finally, IL-6 enhanced the
proliferation (2-fold increase) of IL-2-generated plasmablasts. In
conclusion, the data indicate that IL-6 is a growth factor for
nonmalignant human plasmablasts.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
A. Geffroy-Luseau, G. Jego, R. Bataille, L. Campion, and C. Pellat-Deceunynck
Osteoclasts support the survival of human plasma cells in vitro
Int. Immunol.,
June 1, 2008;
20(6):
775 - 782.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Diehl, H. Schmidlin, M. Nagasawa, S. D. van Haren, M. J. Kwakkenbos, E. Yasuda, T. Beaumont, F. A. Scheeren, and H. Spits
STAT3-Mediated Up-Regulation of BLIMP1 Is Coordinated with BCL6 Down-Regulation to Control Human Plasma Cell Differentiation
J. Immunol.,
April 1, 2008;
180(7):
4805 - 4815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Traggiai, S. Volpi, F. Schena, M. Gattorno, F. Ferlito, L. Moretta, and A. Martini
Bone Marrow-Derived Mesenchymal Stem Cells Induce Both Polyclonal Expansion and Differentiation of B Cells Isolated from Healthy Donors and Systemic Lupus Erythematosus Patients
Stem Cells,
February 1, 2008;
26(2):
562 - 569.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kuchen, R. Robbins, G. P. Sims, C. Sheng, T. M. Phillips, P. E. Lipsky, and R. Ettinger
Essential Role of IL-21 in B Cell Activation, Expansion, and Plasma Cell Generation during CD4+ T Cell-B Cell Collaboration
J. Immunol.,
November 1, 2007;
179(9):
5886 - 5896.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Huggins, T. Pellegrin, R. E. Felgar, C. Wei, M. Brown, B. Zheng, E. C. B. Milner, S. H. Bernstein, I. Sanz, and M. S. Zand
CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells
Blood,
February 15, 2007;
109(4):
1611 - 1619.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Dankerl, P. Liebisch, G. Glatting, C. Friesen, N. M. Blumstein, D. Kocot, C. Wendl, D. Bunjes, and S. N. Reske
Multiple Myeloma: Molecular Imaging with 11C-Methionine PET/CT--Initial Experience
Radiology,
December 19, 2006;
(2006)
2422051980.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
I. Gonzalez-Garcia, E. Ocana, G. Jimenez-Gomez, A. Campos-Caro, and J. A. Brieva
Immunization-Induced Perturbation of Human Blood Plasma Cell Pool: Progressive Maturation, IL-6 Responsiveness, and High PRDI-BF1/BLIMP1 Expression Are Critical Distinctions between Antigen-Specific and Nonspecific Plasma Cells
J. Immunol.,
April 1, 2006;
176(7):
4042 - 4050.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Ettinger, G. P. Sims, A.-M. Fairhurst, R. Robbins, Y. S. da Silva, R. Spolski, W. J. Leonard, and P. E. Lipsky
IL-21 Induces Differentiation of Human Naive and Memory B Cells into Antibody-Secreting Plasma Cells
J. Immunol.,
December 15, 2005;
175(12):
7867 - 7879.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Robillard, C. Pellat-Deceunynck, and R. Bataille
Phenotypic characterization of the human myeloma cell growth fraction
Blood,
June 15, 2005;
105(12):
4845 - 4848.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Zwollo, S. Cole, E. Bromage, and S. Kaattari
B Cell Heterogeneity in the Teleost Kidney: Evidence for a Maturation Gradient from Anterior to Posterior Kidney
J. Immunol.,
June 1, 2005;
174(11):
6608 - 6616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Zhu, G. Somlo, B. Zhou, J. Shao, V. Bedell, M. L. Slovak, X. Liu, J. Luo, and Y. Yen
Fibroblast growth factor receptor 3 inhibition by short hairpin RNAs leads to apoptosis in multiple myeloma
Mol. Cancer Ther.,
May 1, 2005;
4(5):
787 - 798.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. T. Avery, J. I. Ellyard, F. Mackay, L. M. Corcoran, P. D. Hodgkin, and S. G. Tangye
Increased Expression of CD27 on Activated Human Memory B Cells Correlates with Their Commitment to the Plasma Cell Lineage
J. Immunol.,
April 1, 2005;
174(7):
4034 - 4042.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-S. Park, S.-O. Yoon, R. J. Armitage, and Y. S. Choi
Follicular Dendritic Cells Produce IL-15 That Enhances Germinal Center B Cell Proliferation in Membrane-Bound Form
J. Immunol.,
December 1, 2004;
173(11):
6676 - 6683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. O'Nions and M. J. Allday
Proliferation and differentiation in isogenic populations of peripheral B cells activated by Epstein-Barr virus or T cell-derived mitogens
J. Gen. Virol.,
April 1, 2004;
85(4):
881 - 895.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Fitzgerald, S. A. Lee, H. K. Hall, D. S. Chi, and G. Krishnaswamy
Human Lung Fibroblasts Express Interleukin-6 in Response to Signaling after Mast Cell Contact
Am. J. Respir. Cell Mol. Biol.,
April 1, 2004;
30(4):
585 - 593.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. Matsui, C. A. Huff, Q. Wang, M. T. Malehorn, J. Barber, Y. Tanhehco, B. D. Smith, C. I. Civin, and R. J. Jones
Characterization of clonogenic multiple myeloma cells
Blood,
March 15, 2004;
103(6):
2332 - 2336.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Brocke-Heidrich, A. K. Kretzschmar, G. Pfeifer, C. Henze, D. Loffler, D. Koczan, H.-J. Thiesen, R. Burger, M. Gramatzki, and F. Horn
Interleukin-6-dependent gene expression profiles in multiple myeloma INA-6 cells reveal a Bcl-2 family-independent survival pathway closely associated with Stat3 activation
Blood,
January 1, 2004;
103(1):
242 - 251.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Trikha, R. Corringham, B. Klein, and J.-F. Rossi
Targeted Anti-Interleukin-6 Monoclonal Antibody Therapy for Cancer: A Review of the Rationale and Clinical Evidence
Clin. Cancer Res.,
October 15, 2003;
9(13):
4653 - 4665.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tarte, F. Zhan, J. De Vos, B. Klein, and J. Shaughnessy Jr
Gene expression profiling of plasma cells and plasmablasts: toward a better understanding of the late stages of B-cell differentiation
Blood,
July 15, 2003;
102(2):
592 - 600.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Tarte, J. De Vos, T. Thykjaer, F. Zhan, G. Fiol, V. Costes, T. Reme, E. Legouffe, J.-F. Rossi, J. Shaughnessy Jr, et al.
Generation of polyclonal plasmablasts from peripheral blood B cells: a normal counterpart of malignant plasmablasts
Blood,
July 30, 2002;
100(4):
1113 - 1122.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
