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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sellers, S. E. Articles by Sorrentino, B. P. Search for Related Content PubMed PubMed Citation Articles by Sellers, S. E. Articles by Sorrentino, B. P. Related Collections Transplantation Brief Reports Gene Therapy Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 March 2001, Vol. 97, No. 6, pp. 1888-1891 BRIEF REPORT The effect of multidrug-resistance 1 gene versus neo transduction on ex vivo and in vivo expansion of rhesus macaque hematopoietic repopulating cells Stephanie E. Sellers, John F. Tisdale, Brian A. Agricola, Mark E. Metzger, Robert E. Donahue, Cynthia E. Dunbar, and Brian P. Sorrentino From the Hematology Branch, the National Heart, Lung, and Blood Institute, and the Molecular and Clinical Hematology Branch, the National Institute of Diabetes and Digestive and Kidney Diseases, the National Institutes of Health, Bethesda, MD, and the Biochemistry Department, St Jude Children's Research Hospital, Memphis, TN. Transduction of murine stem cells with a multidrug-resistance 1 gene (MDR1) retrovirus results in dramatic ex vivo and in vivo expansion of repopulating cells accompanied by a myeloproliferative disorder. Given the use of MDR1-containing vectors in human trials, investigations have been extended to nonhuman primates. Peripheral blood stem cells from 2 rhesus monkeys were collected, CD34-enriched, split into 2 portions, and transduced with either MDR1 vectors or neo vectors and continued in culture for a total of 10 days before reinfusion. At engraftment, the copy number in granulocytes was extremely high from both MDR vectors and neo vectors, but the copy number fell to 0.01 to 0.05 for both. There were no perturbations of the leukocyte count or differential noted. After 3 cycles of stem cell factor/granulocyte colony-stimulating factor, there were no changes in the levels of MDR1 vector- or neo vector-containing cells. There was no evidence for expansion of MDR1 vector-transduced cells. Long-term engraftment with MDR1 vector- and neo vector-transduced cells occurred despite prolonged culture. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Ahmed, N. Arseni, H. Glimm, W. Hiddemann, C. Buske, and M. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020