SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wright, D. E. Articles by Weissman, I. L. Search for Related Content PubMed PubMed Citation Articles by Wright, D. E. Articles by Weissman, I. L. Related Collections Hematopoiesis and Stem Cells Transplantation Gene Expression Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 April 2001, Vol. 97, No. 8, pp. 2278-2285 HEMATOPOIESIS Cyclophosphamide/granulocyte colony-stimulating factor causes selective mobilization of bone marrow hematopoietic stem cells into the blood after M phase of the cell cycle Douglas E. Wright, Samuel H. Cheshier, Amy J. Wagers, Troy D. Randall, Julie L. Christensen, and Irving L. Weissman From the Departments of Pathology and Developmental Biology, Stanford University School of Medicine, Stanford, California. Cytokine-mobilized peripheral blood hematopoietic stem cells (MPB HSC) are widely used for transplantation in the treatment of malignancies, but the mechanism of HSC mobilization is unclear. Although many HSC in bone marrow (BM) cycle rapidly and expand their numbers in response to cytoreductive agents, such as cyclophosphamide (CY), and cytokines, such as granulocyte colony-stimulating factor (G-CSF), MPB HSC are almost all in the G0 or G1 phase of the cell cycle. This has raised the question of whether a subset of noncycling BM HSC is selectively released, or whether cycling BM HSC are mobilized after M phase, but before the next S phase of the cell cycle. To distinguish between these possibilities, mice were treated with one dose of CY followed by daily doses of G-CSF, and dividing cells were marked by administration of bromodeoxyuridine (BrdU) during the interval that BM HSC are expanding. After CY and 4 days of G-CSF, 98.5% of the 2n DNA content long-term repopulating MPB (LT)-HSC stained positively for BrdU, and therefore derived from cells that divided during the treatment interval. Next, LT-HSC from mice previously treated with a single dose of CY, which kills cycling cells, and 3 daily doses of G-CSF, were nearly all killed by a second dose of CY, suggesting that CY/G-CSF causes virtually all LT-HSC to cycle. Analysis of cyclin D2 messenger RNA (mRNA) expression and total RNA content of MPB HSC suggests that these cells are mostly in G1 phase. After CY/G-CSF treatment, virtually all BM LT-HSC enter the cell cycle; some of these HSC then migrate into the blood, specifically after M phase, and are rapidly recruited to particular hematopoietic organs. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: I. L. Weissman and J. A. Shizuru The origins of the identification and isolation of hematopoietic stem cells, and their capability to induce donor-specific transplantation tolerance and treat autoimmune diseases Blood, November 1, 2008; 112(9): 3543 - 3553. [Abstract] [Full Text] [PDF] S. Shivtiel, O. Kollet, K. Lapid, A. Schajnovitz, P. Goichberg, A. Kalinkovich, E. Shezen, M. Tesio, N. Netzer, I. Petit, et al. CD45 regulates retention, motility, and numbers of hematopoietic progenitors, and affects osteoclast remodeling of metaphyseal trabecules J. Exp. Med., September 29, 2008; 205(10): 2381 - 2395. [Abstract] [Full Text] [PDF] S. R. Mayack and A. J. Wagers Osteolineage niche cells initiate hematopoietic stem cell mobilization Blood, August 1, 2008; 112(3): 519 - 531. [Abstract] [Full Text] [PDF] J. L. Moody, S. Singbrant, G. Karlsson, U. Blank, M. Aspling, J. Flygare, D. Bryder, and S. Karlsson Endoglin Is Not Critical for Hematopoietic Stem Cell Engraftment and Reconstitution but Regulates Adult Erythroid Development Stem Cells, November 1, 2007; 25(11): 2809 - 2819. [Abstract] [Full Text] [PDF] J. P. Chute, G. G. Muramoto, A. B. Salter, S. K. Meadows, D. W. Rickman, B. Chen, H. A. Himburg, and N. J. Chao Transplantation of vascular endothelial cells mediates the hematopoietic recovery and survival of lethally irradiated mice Blood, March 15, 2007; 109(6): 2365 - 2372. [Abstract] [Full Text] [PDF] S. Schenk, N. Mal, A. Finan, M. Zhang, M. Kiedrowski, Z. Popovic, P. M. McCarthy, and M. S. Penn Monocyte Chemotactic Protein-3 Is a Myocardial Mesenchymal Stem Cell Homing Factor Stem Cells, January 1, 2007; 25(1): 245 - 251. [Abstract] [Full Text] [PDF] A. Y. Lai and M. Kondo Asymmetrical lymphoid and myeloid lineage commitment in multipotent hematopoietic progenitors J. Exp. Med., August 7, 2006; 203(8): 1867 - 1873. [Abstract] [Full Text] [PDF] A. J. Wagers and I. L. Weissman Differential Expression of {alpha}2 Integrin Separates Long-Term and Short-Term Reconstituting Lin-/loThy1.1loc-kit+ Sca-1+ Hematopoietic Stem Cells Stem Cells, April 1, 2006; 24(4): 1087 - 1094. [Abstract] [Full Text] [PDF] T. C. Burns, X. R. Ortiz-Gonzalez, M. Gutierrez-Perez, C. D. Keene, R. Sharda, Z. L. Demorest, Y. Jiang, M. Nelson-Holte, M. Soriano, Y. Nakagawa, et al. Thymidine Analogs Are Transferred from Prelabeled Donor to Host Cells in the Central Nervous System After Transplantation: A Word of Caution Stem Cells, April 1, 2006; 24(4): 1121 - 1127. [Abstract] [Full Text] [PDF] E. Passegue, A. J. Wagers, S. Giuriato, W. C. Anderson, and I. L. Weissman Global analysis of proliferation and cell cycle gene expression in the regulation of hematopoietic stem and progenitor cell fates J. Exp. Med., December 5, 2005; 202(11): 1599 - 1611. [Abstract] [Full Text] [PDF] A. Sacco, R. Doyonnas, M. A. LaBarge, M. M. Hammer, P. Kraft, and H. M. Blau IGF-I increases bone marrow contribution to adult skeletal muscle and enhances the fusion of myelomonocytic precursors J. Cell Biol., November 7, 2005; 171(3): 483 - 492. [Abstract] [Full Text] [PDF] A. Y. Lai, S. M. Lin, and M. Kondo Heterogeneity of Flt3-Expressing Multipotent Progenitors in Mouse Bone Marrow J. Immunol., October 15, 2005; 175(8): 5016 - 5023. [Abstract] [Full Text] [PDF] I. Weissman Stem Cell Research: Paths to Cancer Therapies and Regenerative Medicine JAMA, September 21, 2005; 294(11): 1359 - 1366. [Abstract] [Full Text] [PDF] M. A. Grimbaldeston, C.-C. Chen, A. M. Piliponsky, M. Tsai, S.-Y. Tam, and S. J. Galli Mast Cell-Deficient W-sash c-kit Mutant KitW-sh/W-sh Mice as a Model for Investigating Mast Cell Biology in Vivo Am. J. Pathol., September 1, 2005; 167(3): 835 - 848. [Abstract] [Full Text] [PDF] M. Florido, J. E. Pearl, A. Solache, M. Borges, L. Haynes, A. M. Cooper, and R. Appelberg Gamma Interferon-Induced T-Cell Loss in Virulent Mycobacterium avium Infection Infect. Immun., June 1, 2005; 73(6): 3577 - 3586. [Abstract] [Full Text] [PDF] M. Massengale, A. J. Wagers, H. Vogel, and I. L. Weissman Hematopoietic cells maintain hematopoietic fates upon entering the brain J. Exp. Med., May 16, 2005; 201(10): 1579 - 1589. [Abstract] [Full Text] [PDF] R. I. Sherwood, J. L. Christensen, I. L. Weissman, and A. J. Wagers Determinants of Skeletal Muscle Contributions from Circulating Cells, Bone Marrow Cells, and Hematopoietic Stem Cells Stem Cells, December 1, 2004; 22(7): 1292 - 1304. [Abstract] [Full Text] [PDF] F. Togel, J. Isaac, and C. Westenfelder Hematopoietic Stem Cell Mobilization-Associated Granulocytosis Severely Worsens Acute Renal Failure J. Am. Soc. Nephrol., May 1, 2004; 15(5): 1261 - 1267. [Abstract] [Full Text] [PDF] C. R. D. Carter, K. M. Whitmore, and R. Thorpe The significance of carbohydrates on G-CSF: differential sensitivity of G-CSFs to human neutrophil elastase degradation J. Leukoc. Biol., March 1, 2004; 75(3): 515 - 522. [Abstract] [Full Text] [PDF] Y.-A. Cao, A. J. Wagers, A. Beilhack, J. Dusich, M. H. Bachmann, R. S. Negrin, I. L. Weissman, and C. H. Contag Shifting foci of hematopoiesis during reconstitution from single stem cells PNAS, January 6, 2004; 101(1): 221 - 226. [Abstract] [Full Text] [PDF] R. Sakai, T. Kajiume, H. Inoue, R. Kanno, M. Miyazaki, Y. Ninomiya, and M. Kanno TCDD Treatment Eliminates the Long-Term Reconstitution Activity of Hematopoietic Stem Cells Toxicol. Sci., March 1, 2003; 72(1): 84 - 91. [Abstract] [Full Text] [PDF] T. N. Nakorn, T. Miyamoto, and I. L. Weissman Characterization of mouse clonogenic megakaryocyte progenitors PNAS, January 7, 2003; 100(1): 205 - 210. [Abstract] [Full Text] [PDF] K. L. Kusser and T. D. Randall Simultaneous Detection of EGFP and Cell Surface Markers by Fluorescence Microscopy in Lymphoid Tissues J. Histochem. Cytochem., January 1, 2003; 51(1): 5 - 14. [Abstract] [Full Text] [PDF] A. J. Wagers, R. I. Sherwood, J. L. Christensen, and I. L. Weissman Little Evidence for Developmental Plasticity of Adult Hematopoietic Stem Cells Science, September 27, 2002; 297(5590): 2256 - 2259. [Abstract] [Full Text] [PDF] S. Huygen, O. Giet, V. Artisien, I. Di Stefano, Y. Beguin, and A. Gothot Adhesion of synchronized human hematopoietic progenitor cells to fibronectin and vascular cell adhesion molecule-1 fluctuates reversibly during cell cycle transit in ex vivo culture Blood, September 26, 2002; 100(8): 2744 - 2752. [Abstract] [Full Text] [PDF] E. Henckaerts, H. Geiger, J. C. Langer, P. Rebollo, G. Van Zant, and H.-W. Snoeck Genetically determined variation in the number of phenotypically defined hematopoietic progenitor and stem cells and in their response to early-acting cytokines Blood, May 13, 2002; 99(11): 3947 - 3954. [Abstract] [Full Text] [PDF] D. E. Wright, E. P. Bowman, A. J. Wagers, E. C. Butcher, and I. L. Weissman Hematopoietic Stem Cells Are Uniquely Selective in Their Migratory Response to Chemokines J. Exp. Med., May 6, 2002; 195(9): 1145 - 1154. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020