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Blood, 1 May 2001, Vol. 97, No. 9, pp. 2808-2814
NEOPLASIA
Phagocytic dendritic cells from myelomas activate tumor-specific
T cells at a single cell level
Zlatko Dembic,
John-Arne Røttingen,
Jérôme Dellacasagrande,
Karl Schenck, and
Bjarne Bogen
From the Institute of Immunology, Department of
Physiology, and Department of Oral Biology, University of Oslo, Oslo,
Norway.
Antigen-presenting cells (APCs) from subcutaneous mouse
MOPC315 plasmacytoma phagocytosed immunoglobulin G-coated magnetic beads, enabling efficient isolation within 2 hours by magnetic separation (APC-MB). Cell morphology was heterogeneous, with some of
the cells having dendrites. The surface phenotype of purified tumor
APCs-MB was CD11b+, CD11c+, CD40+,
CD80+, CD86+, and MHC class II+.
Tumor APCs-MB expressed messenger RNA for fractalkine and ABCD-1 chemokines, and for CC-type chemokine receptors CCR5 and CCR7, indicating the presence of mature dendritic cells (DCs). Visualized at
a single cell level within 4 hours after disruption of the tumor,
APCs-MB induced rapid Ca++ mobilization in MHC class
II-restricted tumor idiotype (Id)-specific cloned CD4+ T
cells. In long-term assays, tumor APCs-MB induced proliferation of
naive T cells from Id-specific T-cell receptor transgenic mice. The
results suggest that tumor APCs-MB represent a heterogeneous cell
population that includes myeloid-derived DCs of various stages of
maturation. A considerable fraction ( 15%) of DCs is spontaneously primed with tumor-specific antigen.
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