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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Dembic, Z. Articles by Bogen, B. Search for Related Content PubMed PubMed Citation Articles by Dembic, Z. Articles by Bogen, B. Related Collections Immunobiology Neoplasia Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 May 2001, Vol. 97, No. 9, pp. 2808-2814 NEOPLASIA Phagocytic dendritic cells from myelomas activate tumor-specific T cells at a single cell level Zlatko Dembic, John-Arne Røttingen, Jérôme Dellacasagrande, Karl Schenck, and Bjarne Bogen From the Institute of Immunology, Department of Physiology, and Department of Oral Biology, University of Oslo, Oslo, Norway. Antigen-presenting cells (APCs) from subcutaneous mouse MOPC315 plasmacytoma phagocytosed immunoglobulin G-coated magnetic beads, enabling efficient isolation within 2 hours by magnetic separation (APC-MB). Cell morphology was heterogeneous, with some of the cells having dendrites. The surface phenotype of purified tumor APCs-MB was CD11b+, CD11c+, CD40+, CD80+, CD86+, and MHC class II+. Tumor APCs-MB expressed messenger RNA for fractalkine and ABCD-1 chemokines, and for CC-type chemokine receptors CCR5 and CCR7, indicating the presence of mature dendritic cells (DCs). Visualized at a single cell level within 4 hours after disruption of the tumor, APCs-MB induced rapid Ca++ mobilization in MHC class II-restricted tumor idiotype (Id)-specific cloned CD4+ T cells. In long-term assays, tumor APCs-MB induced proliferation of naive T cells from Id-specific T-cell receptor transgenic mice. The results suggest that tumor APCs-MB represent a heterogeneous cell population that includes myeloid-derived DCs of various stages of maturation. A considerable fraction ( 15%) of DCs is spontaneously primed with tumor-specific antigen. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Corthay, K. U. Lundin, K. B. Lorvik, P. O. Hofgaard, and B. Bogen Secretion of Tumor-Specific Antigen by Myeloma Cells Is Required for Cancer Immunosurveillance by CD4+ T Cells Cancer Res., July 15, 2009; 69(14): 5901 - 5907. [Abstract] [Full Text] [PDF] Y. Maeda, T. Yoshino, M. Takahashi, H. Ginya, J. Asahina, H. Tajima, and T. Matsunaga Noncovalent Immobilization of Streptavidin on In Vitro- and In Vivo-Biotinylated Bacterial Magnetic Particles Appl. Envir. Microbiol., August 15, 2008; 74(16): 5139 - 5145. [Abstract] [Full Text] [PDF] L. A. Munthe, A. Os, M. Zangani, and B. Bogen MHC-Restricted Ig V Region-Driven T-B Lymphocyte Collaboration: B Cell Receptor Ligation Facilitates Switch to IgG Production J. Immunol., June 15, 2004; 172(12): 7476 - 7484. [Abstract] [Full Text] [PDF] K. U. Lundin, P. O. Hofgaard, H. Omholt, L. A. Munthe, A. Corthay, and B. Bogen Therapeutic effect of idiotype-specific CD4+ T cells against B-cell lymphoma in the absence of anti-idiotypic antibodies Blood, July 15, 2003; 102(2): 605 - 612. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020