SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Bae, Y.-S. Articles by Ryu, S. H. Search for Related Content PubMed PubMed Citation Articles by Bae, Y.-S. Articles by Ryu, S. H. Related Collections Phagocytes Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 May 2001, Vol. 97, No. 9, pp. 2854-2862 PHAGOCYTES Identification of novel chemoattractant peptides for human leukocytes Yoe-Sik Bae, Hyunjoo Bae, Youndong Kim, Taehoon G. Lee, Pann-Ghill Suh, and Sung Ho Ryu From the Division of Molecular and Life Sciences, Pohang University of Science and Technology, Korea. Superoxide is the most important armory on the primary defense line of monocytes against invading pathogens, and the identification of new stimuli and the characterization of the regulatory mechanism of superoxide generation are of paramount importance. In this study, we identified 3 novel peptides by screening a synthetic hexapeptide combinatorial library and modification of 1 of the peptides. The isolated peptides that can induce superoxide generation in human monocytes are His-Phe-Tyr-Leu-Pro-Met-CONH2 (HFYLPM), Met-Phe-Tyr-Leu-Pro-Met-CONH2 (MFYLPM), and His-Phe-Tyr-Leu-Pro-D-Met-CONH2 (HFYLPm). All 3 peptides also caused intracellular calcium ([Ca++]i) rise. We tested the specificities of the peptides on cells of different origin by looking at [Ca++]i rise. All 3 peptides acted specifically on leukocytes and not on nonimmune cells. Among leukocytes, HL60 and Jurkat T cells were stimulated specifically by MFYLPM or HFYLPM, respectively. As a physiologic characteristic of the peptides, we observed that all 3 peptides induced chemotactic migration of monocytes. Studying receptor specificity, we concluded that the 3 peptides might act on some shared and some distinct receptor(s) on leukocytes. Studying intracellular signaling set in motion by the peptides revealed that HFYLPM, but not MFYLPM or HFYLPm, induced chemotaxis via phospatidylinositol-3 kinase and protein kinase C. Because HFYLPM, MFYLPM, and HFYLPm not only exhibit different specificities depending on cell type and status of differentiation but also stimulate cells via distinct receptors and signaling, the 3 novel peptides might be useful tools to study leukocyte activation. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. K. Lim, W. Lu, O. Hartley, and A. L. DeVico N-terminal proteolytic processing by cathepsin G converts RANTES/CCL5 and related analogs into a truncated 4-68 variant J. Leukoc. Biol., December 1, 2006; 80(6): 1395 - 1404. [Abstract] [Full Text] [PDF] H. Y. Lee, M.-K. Kim, K. S. Park, E. H. Shin, S. H. Jo, S. D. Kim, E. J. Jo, Y.-N. Lee, C. Lee, S.-H. Baek, et al. Serum Amyloid A Induces Contrary Immune Responses via Formyl Peptide Receptor-Like 1 in Human Monocytes Mol. Pharmacol., July 1, 2006; 70(1): 241 - 248. [Abstract] [Full Text] [PDF] Y. Kim, B. D. Lee, O. Kim, Y.-S. Bae, T. Lee, P.-G. Suh, and S. H. Ryu Pituitary adenylate cyclase-activating polypeptide 27 is a functional ligand for formyl Peptide receptor-like 1. J. Immunol., March 1, 2006; 176(5): 2969 - 2975. [Abstract] [Full Text] [PDF] K. S. Park, H.-Y. Lee, M.-K. Kim, E. H. Shin, S. H. Jo, S. D. Kim, D.-S. Im, and Y.-S. Bae Lysophosphatidylserine Stimulates L2071 Mouse Fibroblast Chemotactic Migration via a Process Involving Pertussis Toxin-Sensitive Trimeric G-Proteins Mol. Pharmacol., March 1, 2006; 69(3): 1066 - 1073. [Abstract] [Full Text] [PDF] J. K. Lim, J. M. Burns, W. Lu, and A. L. DeVico Multiple pathways of amino terminal processing produce two truncated variants of RANTES/CCL5 J. Leukoc. Biol., August 1, 2005; 78(2): 442 - 452. [Abstract] [Full Text] [PDF] H. K. Kang, H.-Y. Lee, M.-K. Kim, K. S. Park, Y. M. Park, J.-Y. Kwak, and Y.-S. Bae The Synthetic Peptide Trp-Lys-Tyr-Met-Val-D-Met Inhibits Human Monocyte-Derived Dendritic Cell Maturation via Formyl Peptide Receptor and Formyl Peptide Receptor-Like 2 J. Immunol., July 15, 2005; 175(2): 685 - 692. [Abstract] [Full Text] [PDF] E. J. Jo, H.-Y. Lee, Y.-N. Lee, J. I. Kim, H.-K. Kang, D.-W. Park, S.-H. Baek, J.-Y. Kwak, and Y.-S. Bae Group IB Secretory Phospholipase A2 Stimulates CXC Chemokine Ligand 8 Production via ERK and NF-{kappa}B in Human Neutrophils J. Immunol., November 15, 2004; 173(10): 6433 - 6439. [Abstract] [Full Text] [PDF] Y.-S. Bae, H. Y. Lee, E. J. Jo, J. I. Kim, H.-K. Kang, R. D. Ye, J.-Y. Kwak, and S. H. Ryu Identification of Peptides That Antagonize Formyl Peptide Receptor-Like 1-Mediated Signaling J. Immunol., July 1, 2004; 173(1): 607 - 614. [Abstract] [Full Text] [PDF] Y.-S. Bae, H. J. Yi, H.-Y. Lee, E. J. Jo, J. I. Kim, T. G. Lee, R. D. Ye, J.-Y. Kwak, and S. H. Ryu Differential Activation of Formyl Peptide Receptor-Like 1 by Peptide Ligands J. Immunol., December 15, 2003; 171(12): 6807 - 6813. [Abstract] [Full Text] [PDF] Y.-S. Bae, J. C. Park, R. He, R. D. Ye, J.-Y. Kwak, P.-G. Suh, and S. Ho Ryu Differential Signaling of Formyl Peptide Receptor-Like 1 by Trp-Lys-Tyr-Met-Val-Met-CONH2 or Lipoxin A4 in Human Neutrophils Mol. Pharmacol., September 1, 2003; 64(3): 721 - 730. [Abstract] [Full Text] [PDF] Y.-S. Bae, T. G. Lee, J. C. Park, J. H. Hur, Y. Kim, K. Heo, J.-Y. Kwak, P.-G. Suh, and S. H. Ryu Identification of a Compound That Directly Stimulates Phospholipase C Activity Mol. Pharmacol., May 1, 2003; 63(5): 1043 - 1050. [Abstract] [Full Text] [PDF]

	Blood Online is supported in part by Genentech BioOncology and Biogen Idec
	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020