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Related Collections Immunobiology Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 July 2001, Vol. 98, No. 1, pp. 156-164 IMMUNOBIOLOGY CD8 T cells specific for human immunodeficiency virus, Epstein-Barr virus, and cytomegalovirus lack molecules for homing to lymphoid sites of infection Gang Chen, Premlata Shankar, Christoph Lange, Hernan Valdez, Paul R. Skolnik, Lijun Wu, N. Manjunath, and Judy Lieberman From the Center for Blood Research and Department of Pediatrics, Harvard Medical School, Boston, MA; Division of Infectious Diseases, Department of Medicine, University Hospitals of Cleveland, Case Western Reserve University, Cleveland, OH; Division of Infectious Diseases and Geographic Medicine, Department of Medicine, New England Medical Center, Tufts University School of Medicine, Boston, MA; and Millennium Pharmaceuticals, Inc, Cambridge, MA. CD8 T cells are classified as naïve, effector, or memory cells on the basis of CD45RA, CD62L, and CCR7 expression. Sequential engagement of cell-surface CD62L and CCR7 receptors is required for efficient trafficking to lymphoid tissue by means of high endothelial venules. Naïve CD8 T cells are CCR7+CD62L+ CD45RA+, whereas long-term memory cells are CCR7+CD62L+CD45RA. Effector cytotoxic T cells are thought to be CCR7CD45RA+. The distribution of CD8 subsets and cytolytic protein expression in healthy donors and donors seropositive for human immunodeficiency virus (HIV) were compared. In HIV-infected subjects, CCR7 CD8 T cells expanded at the expense of naïve and long-term memory cells. In both healthy donors and HIV-infected donors, CCR7+ CD8 T cells were uniformly negative for perforin. In all subsets, perforin and granzyme A were not coordinately expressed, with perforin expression being more tightly regulated. The properties of CD8 T cells specific for cytomegalovirus, Epstein-Barr virus (EBV), and HIV were studied by staining with major histocompatibility complex peptide tetramers. Antigen-specific cells for chronic infections with these viruses were uniformly CCR7 and predominantly CD62L. In 2 HIV-seropositive donors, 3- to 4-fold fewer EBV-tetramer-positive cells were present in lymph nodes compared with blood. Antigen-specific CD8 T cells are therefore preferentially excluded from lymphoid sites, even when infection is primarily in lymphoid tissue. This may protect lymphoid tissues from immunopathological changes but compromise immune defense against viruses, such as HIV and EBV, that target lymphocytes. HIV-specific CD8 T cells do not express CD45RA, whereas EBV- and CMV-specific CD8 T cells are heterogeneous in CD45RA+ expression. Lack of CD45RA expression may indicate incomplete differentiation of HIV-specific CD8 T cells to cytotoxic T cells. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. M. Iancu, P. Corthesy, P. Baumgaertner, E. Devevre, V. Voelter, P. Romero, D. E. Speiser, and N. Rufer Clonotype Selection and Composition of Human CD8 T Cells Specific for Persistent Herpes Viruses Varies with Differentiation but Is Stable Over Time J. Immunol., July 1, 2009; 183(1): 319 - 331. [Abstract] [Full Text] [PDF] S. S. Cush and E. Flano Protective Antigen-Independent CD8 T Cell Memory Is Maintained during {gamma}-Herpesvirus Persistence J. Immunol., April 1, 2009; 182(7): 3995 - 4004. [Abstract] [Full Text] [PDF] A. H. Sabouri, K. Usuku, D. Hayashi, S. Izumo, Y. Ohara, M. Osame, and M. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020