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Blood, 1 July 2001, Vol. 98, No. 1, pp. 93-99
HEMATOPOIESIS
Highly abundant genes in the transcriptosome of human
and baboon CD34 antigen-positive bone marrow cells
Ignatius Gomes,
Tiffany T. Sharma,
Nadim Mahmud,
Jeffrey D. Kapp,
Seby Edassery,
Noreen Fulton,
Jie Liang,
Ronald Hoffman, and
Carol A. Westbrook
From the Department of Medicine and Department of
Bioengineering, University of Illinois at Chicago.
Nonhuman primates are useful large animal model systems for the in
vivo study of hematopoietic stem cell biology. To better understand the
degree of similarity of the hematopoietic systems between humans and
baboons, and to explore the relevance of such studies in nonhuman
primates to humans, this study was designed to compare the global gene
expression profile of bone marrow CD34+ cells isolated from
these 2 species. Human complementary DNA (cDNA) filter arrays
containing 25 920 human cDNAs were surveyed for this purpose. The
expression pattern and relative gene abundance of the 2 RNA sources
were similar, with a correlation coefficient of 0.87. A total of
15 970 of these cDNAs were expressed in human CD34+ cells,
of which the majority (96%) varied less than 3-fold in their relative
level of expression between human and baboon. Reverse transcriptase-polymerase chain reaction analysis of selected genes confirmed that expression was comparable between the 2 species. No
species-restricted transcripts have been identified, further reinforcing the high degree of similarity between the 2 populations. A
subset of 1554 cDNAs, which are expressed at levels 100-fold and
greater than background, is described, which includes 959 expressed
sequence tags and uncharacterized cDNAs, and 595 named genes, including many that are clearly involved in hematopoiesis. The cDNAs reported here represent a selection of some of the most highly abundant genes in hematopoietic cells and provide a starting point to develop a profile of the transcriptosome of CD34+ cells.
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