|
|
 Previous Article | Table of Contents | Next Article 
Blood, 1 December 2001, Vol. 98, No. 12, pp. 3283-3289
HEMATOPOIESIS
Notch1 inhibits differentiation of hematopoietic
cells by sustaining GATA-2 expression
Keiki Kumano,
Shigeru Chiba,
Kiyoshi Shimizu,
Tetsuya Yamagata,
Noriko Hosoya,
Toshiki Saito,
Tokiharu Takahashi,
Yoshio Hamada, and
Hisamaru Hirai
From the Department of Hematology and Oncology,
Graduate School of Medicine, University of Tokyo, Tokyo, Japan and the
National Institute of Basic Biology, Okazaki, Japan.
Notch signaling is involved in cell fate decisions in many systems
including hematopoiesis. It has been shown that expression of an
activated form of Notch1 (aNotch1) in 32D mouse myeloid progenitor
cells inhibits the granulocytic differentiation induced by granulocyte
colony-stimulating factor (G-CSF). Results of the current study show
that aNotch1, when expressed in F5-5 mouse erythroleukemia cells, also
inhibits erythroid differentiation. Comparison of the expression
levels of several transcription factors after stimulation for
myeloid and erythroid differentiation, in the presence or absence of
aNotch1, revealed that aNotch1 did not change its regulation pattern
with any of the transcription factors examined, except for GATA-2,
despite its inhibitory effect on differentiation. GATA-2 was
down-regulated when the parental 32D and F5-5 were induced to
differentiate into granulocytic and erythroid lineages, respectively.
In these induction procedures, however, the level of GATA-2 expression
was sustained when aNotch1 was expressed. To ascertain whether
maintenance of GATA-2 is required for the Notch-induced inhibition of
differentiation, the dominant-negative form of GATA-3 (DN-GATA), which
acted also against GATA-2, or transcription factor PU.1, which was
recently shown to be the repressor of GATA-2, was introduced
into aNotch1-expressing 32D (32D/aNotch1) cells that do not express
GATA family proteins other than GATA2. Both DN-GATA and PU.1 reversed
the phenotype of 32D/aNotch1 inducing its differentiation when G-CSF
was added. Furthermore, enforced expression of HES-1, which is involved
in Notch signaling, delayed differentiation of 32D, and again this
phenotype was neutralized by DN-GATA. These results indicate that
GATA-2 activity is necessary for the Notch signaling in hematopoietic cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. Sakata-Yanagimoto, E. Nakagami-Yamaguchi, T. Saito, K. Kumano, K. Yasutomo, S. Ogawa, M. Kurokawa, and S. Chiba
From the Cover: Coordinated regulation of transcription factors through Notch2 is an important mediator of mast cell fate
PNAS,
June 3, 2008;
105(22):
7839 - 7844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Brunet de la Grange, E. Zink, F. Armstrong, M.-C. Rouyez, and F. Pflumio
Impairment of Granulo-Monocytic Development of Human Common Myeloid Progenitors but Not of Granulo-Monocytic Progenitors by Decreasing Stem Cell Leukemia/T-Cell Acute Leukemia 1 Expression
Stem Cells,
June 1, 2008;
26(6):
1658 - 1662.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Koga, N. Yamaguchi, T. Abe, M. Minegishi, S. Tsuchiya, M. Yamamoto, and N. Minegishi
Cell-cycle-dependent oscillation of GATA2 expression in hematopoietic cells
Blood,
May 15, 2007;
109(10):
4200 - 4208.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. F. de Pooter, T. M. Schmitt, J. L. de la Pompa, Y. Fujiwara, S. H. Orkin, and J. C. Zuniga-Pflucker
Notch Signaling Requires GATA-2 to Inhibit Myelopoiesis from Embryonic Stem Cells and Primary Hemopoietic Progenitors
J. Immunol.,
May 1, 2006;
176(9):
5267 - 5275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Neves, F. Weerkamp, A. C. Gomes, B. A. E. Naber, P. Gameiro, J. D. Becker, P. Lucio, N. Clode, J. J. M. Van Dongen, F. J. T. Staal, et al.
Effects of Delta1 and Jagged1 on Early Human Hematopoiesis: Correlation with Expression of Notch Signaling-Related Genes in CD34+ Cells
Stem Cells,
May 1, 2006;
24(5):
1328 - 1337.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Garces and S. Thor
Specification of Drosophila aCC motoneuron identity by a genetic cascade involving even-skipped, grain and zfh1
Development,
April 15, 2006;
133(8):
1445 - 1455.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Maki, T. Yamagata, T. Asai, I. Yamazaki, H. Oda, H. Hirai, and K. Mitani
Dysplastic definitive hematopoiesis in AML1/EVI1 knock-in embryos
Blood,
September 15, 2005;
106(6):
2147 - 2155.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Yatsula, S. Lin, A. J. Read, A. Poholek, K. Yates, D. Yue, P. Hui, and A. S. Perkins
Identification of Binding Sites of EVI1 in Mammalian Cells
J. Biol. Chem.,
September 2, 2005;
280(35):
30712 - 30722.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. N. Taghon, E.-S. David, J. C. Zuniga-Pflucker, and E. V. Rothenberg
Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling
Genes & Dev.,
April 15, 2005;
19(8):
965 - 978.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Robert-Moreno, L. Espinosa, J. L. de la Pompa, and A. Bigas
RBPj{kappa}-dependent Notch function regulates Gata2 and is essential for the formation of intra-embryonic hematopoietic cells
Development,
March 1, 2005;
132(5):
1117 - 1126.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Ishiko, I. Matsumura, S. Ezoe, K. Gale, J. Ishiko, Y. Satoh, H. Tanaka, H. Shibayama, M. Mizuki, T. Era, et al.
Notch Signals Inhibit the Development of Erythroid/Megakaryocytic Cells by Suppressing GATA-1 Activity through the Induction of HES1
J. Biol. Chem.,
February 11, 2005;
280(6):
4929 - 4939.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. K. Hadland, S. S. Huppert, J. Kanungo, Y. Xue, R. Jiang, T. Gridley, R. A. Conlon, A. M. Cheng, R. Kopan, and G. D. Longmore
A requirement for Notch1 distinguishes 2 phases of definitive hematopoiesis during development
Blood,
November 15, 2004;
104(10):
3097 - 3105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. M. Vercauteren and H. J. Sutherland
Constitutively active Notch4 promotes early human hematopoietic progenitor cell maintenance while inhibiting differentiation and causes lymphoid abnormalities in vivo
Blood,
October 15, 2004;
104(8):
2315 - 2322.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. E. Elagib, M. Xiao, I. M. Hussaini, L. L. Delehanty, L. A. Palmer, F. K. Racke, M. J. Birrer, G. Shanmugasundaram, M. A. McDevitt, and A. N. Goldfarb
Jun Blockade of Erythropoiesis: Role for Repression of GATA-1 by HERP2
Mol. Cell. Biol.,
September 1, 2004;
24(17):
7779 - 7794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Bullinger, K. Dohner, E. Bair, S. Frohling, R. F. Schlenk, R. Tibshirani, H. Dohner, and J. R. Pollack
Use of Gene-Expression Profiling to Identify Prognostic Subclasses in Adult Acute Myeloid Leukemia
N. Engl. J. Med.,
April 15, 2004;
350(16):
1605 - 1616.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Duan, F.-Q. Li, J. Wechsler, K. Meade-White, K. Williams, K. F. Benson, and M. Horwitz
A Novel Notch Protein, N2N, Targeted by Neutrophil Elastase and Implicated in Hereditary Neutropenia
Mol. Cell. Biol.,
January 1, 2004;
24(1):
58 - 70.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Tavor, D. J. Park, S. Gery, P. T. Vuong, A. F. Gombart, and H. P. Koeffler
Restoration of C/EBP{alpha} Expression in a BCR-ABL+ Cell Line Induces Terminal Granulocytic Differentiation
J. Biol. Chem.,
December 26, 2003;
278(52):
52651 - 52659.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Palaga, L. Miele, T. E. Golde, and B. A. Osborne
TCR-Mediated Notch Signaling Regulates Proliferation and IFN-{gamma} Production in Peripheral T Cells
J. Immunol.,
September 15, 2003;
171(6):
3019 - 3024.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Magrangeas, V. Nasser, H. Avet-Loiseau, B. Loriod, O. Decaux, S. Granjeaud, F. Bertucci, D. Birnbaum, C. Nguyen, J.-L. Harousseau, et al.
Gene expression profiling of multiple myeloma reveals molecular portraits in relation to the pathogenesis of the disease
Blood,
June 15, 2003;
101(12):
4998 - 5006.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-F. Lambert, M. Liu, G. A. Colvin, M. Dooner, C. I. McAuliffe, P. S. Becker, B. G. Forget, S. M. Weissman, and P. J. Quesenberry
Marrow Stem Cells Shift Gene Expression and Engraftment Phenotype with Cell Cycle Transit
J. Exp. Med.,
June 2, 2003;
197(11):
1563 - 1572.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yamada, H. Yamazaki, T. Yamane, M. Yoshino, H. Okuyama, M. Tsuneto, T. Kurino, S.-I. Hayashi, and S. Sakano
Regulation of osteoclast development by Notch signaling directed to osteoclast precursors and through stromal cells
Blood,
March 15, 2003;
101(6):
2227 - 2234.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Varnum-Finney, C. Brashem-Stein, and I. D. Bernstein
Combined effects of Notch signaling and cytokines induce a multiple log increase in precursors with lymphoid and myeloid reconstituting ability
Blood,
March 1, 2003;
101(5):
1784 - 1789.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Espinosa, J. Ingles-Esteve, A. Robert-Moreno, and A. Bigas
Ikappa Balpha and p65 Regulate the Cytoplasmic Shuttling of Nuclear Corepressors: Cross-talk between Notch and NFkappa B Pathways
Mol. Biol. Cell,
February 1, 2003;
14(2):
491 - 502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Schuster, K. Forster, H. Dierks, A. Elsasser, G. Behre, N. Simon, S. Danhauser-Riedl, M. Hallek, and M. Warmuth
The effects of Bcr-Abl on C/EBP transcription-factor regulation and neutrophilic differentiation are reversed by the Abl kinase inhibitor imatinib mesylate
Blood,
January 15, 2003;
101(2):
655 - 663.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
