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Related Collections Hemostasis, Thrombosis, and Vascular Biology Cell Adhesion and Motility Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 December 2001, Vol. 98, No. 13, pp. 3677-3684 HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Identification of CD146 as a component of the endothelial junction involved in the control of cell-cell cohesion Nathalie Bardin, Francine Anfosso, Jean-Marc Massé, Elisabeth Cramer, Florence Sabatier, André Le Bivic, José Sampol, and Françoise Dignat-George From INSERM EMI 0019, Physiopathologie de l'Endothélium, Université de la Méditerranée, UFR Pharmacie, Marseille, France; INSERM U474, ICGM, Hôpital de Port-Royal, Paris, France; and IBDM, Faculté des Sciences de Luminy, Marseille, France. CD146 is a cell-surface molecule belonging to the immunoglobulin superfamily and expressed in all types of human endothelial cells. Confocal and electron microscopic analysis of confluent human umbilical vein endothelial cells (HUVECs) were used to demonstrate that CD146 is a component of the endothelial junction. Double immunolabeling with vascular endothelial cadherin showed that CD146 is localized outside the adherens junction. Moreover, CD146 expression is not restricted to the junction, since part of the labeling was detectable at the apical side of the HUVECs. Interestingly, cell-surface expression of CD146 increased when HUVECs reached confluence. In addition, the paracellular permeability of CD146-transfected fibroblast cells was decreased compared with that of control cells. Finally, CD146 colocalized with actin, was partly resistant to Triton X-100 extraction, and had its expression altered by actin-disrupting agents, indicating that CD146 is associated with the actin cytoskeleton. These results show the regulated expression of CD146 at areas of cell-cell junction and strongly suggest involvement of CD146 as a mediator of cell-cell interaction. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. Zhang, L. Li, L. Feng, Y. Zhang, X. Zeng, J. Feng, D. Yang, C. Zheng, and X. Yan Elevated Levels of Soluble and Neutrophil CD146 in Active Systemic Vasculitis Lab Med, June 1, 2009; 40(6): 351 - 356. [Abstract] [Full Text] [PDF] N. Bardin, M. Blot-Chabaud, N. Despoix, A. Kebir, K. Harhouri, J.-P. Arsanto, L. Espinosa, P. Perrin, S. Robert, F. Vely, et al. CD146 and its Soluble Form Regulate Monocyte Transendothelial Migration Arterioscler. Thromb. Vasc. Biol., May 1, 2009; 29(5): 746 - 753. [Abstract] [Full Text] [PDF] Z. Touat, L. Lepage, V. Ollivier, P. Nataf, U. Hvass, J. Labreuche, M. Jandrot-Perrus, J.-B. Michel, and G. 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