|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 December 2001, Vol. 98, No. 13, pp. 3677-3684
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Identification of CD146 as a component of the endothelial
junction involved in the control of cell-cell cohesion
Nathalie Bardin,
Francine Anfosso,
Jean-Marc Massé,
Elisabeth Cramer,
Florence Sabatier,
André Le Bivic,
José Sampol, and
Françoise Dignat-George
From INSERM EMI 0019, Physiopathologie de
l'Endothélium, Université de la Méditerranée,
UFR Pharmacie, Marseille, France; INSERM U474, ICGM, Hôpital de
Port-Royal, Paris, France; and IBDM, Faculté des Sciences de
Luminy, Marseille, France.
CD146 is a cell-surface molecule belonging to the immunoglobulin
superfamily and expressed in all types of human endothelial cells.
Confocal and electron microscopic analysis of confluent human umbilical
vein endothelial cells (HUVECs) were used to demonstrate that
CD146 is a component of the endothelial junction. Double immunolabeling
with vascular endothelial cadherin showed that CD146 is localized
outside the adherens junction. Moreover, CD146 expression is not
restricted to the junction, since part of the labeling was detectable
at the apical side of the HUVECs. Interestingly, cell-surface
expression of CD146 increased when HUVECs reached confluence. In
addition, the paracellular permeability of CD146-transfected fibroblast
cells was decreased compared with that of control cells. Finally, CD146
colocalized with actin, was partly resistant to Triton X-100
extraction, and had its expression altered by actin-disrupting agents,
indicating that CD146 is associated with the actin cytoskeleton. These
results show the regulated expression of CD146 at areas of cell-cell
junction and strongly suggest involvement of CD146 as a mediator of
cell-cell interaction.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
Z. Touat, L. Lepage, V. Ollivier, P. Nataf, U. Hvass, J. Labreuche, M. Jandrot-Perrus, J.-B. Michel, and G. Jondeau
Dilation-Dependent Activation of Platelets and Prothrombin in Human Thoracic Ascending Aortic Aneurysm
Arterioscler. Thromb. Vasc. Biol.,
May 1, 2008;
28(5):
940 - 946.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Guezguez, P. Vigneron, N. Lamerant, C. Kieda, T. Jaffredo, and D. Dunon
Dual Role of Melanoma Cell Adhesion Molecule (MCAM)/CD146 in Lymphocyte Endothelium Interaction: MCAM/CD146 Promotes Rolling via Microvilli Induction in Lymphocyte and Is an Endothelial Adhesion Receptor
J. Immunol.,
November 15, 2007;
179(10):
6673 - 6685.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bouyssie, A. G. de Peredo, E. Mouton, R. Albigot, L. Roussel, N. Ortega, C. Cayrol, O. Burlet-Schiltz, J.-P. Girard, and B. Monsarrat
Mascot File Parsing and Quantification (MFPaQ), a New Software to Parse, Validate, and Quantify Proteomics Data Generated by ICAT and SILAC Mass Spectrometric Analyses: Application To the Proteomics Study of Membrane Proteins from Primary Human Endothelial Cells
Mol. Cell. Proteomics,
September 1, 2007;
6(9):
1621 - 1637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Schmelzer, L. Zhang, A. Bruce, E. Wauthier, J. Ludlow, H.-l. Yao, N. Moss, A. Melhem, R. McClelland, W. Turner, et al.
Human hepatic stem cells from fetal and postnatal donors
J. Exp. Med.,
August 6, 2007;
204(8):
1973 - 1987.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Shasby
Cell-cell adhesion in lung endothelium
Am J Physiol Lung Cell Mol Physiol,
March 1, 2007;
292(3):
L593 - L607.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. J. Boos, G. Y.H. Lip, and A. D. Blann
Circulating Endothelial Cells in Cardiovascular Disease
J. Am. Coll. Cardiol.,
October 17, 2006;
48(8):
1538 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Duda, K. S. Cohen, E. di Tomaso, P. Au, R. J. Klein, D. T. Scadden, C. G. Willett, and R. K. Jain
Differential CD146 Expression on Circulating Versus Tissue Endothelial Cells in Rectal Cancer Patients: Implications for Circulating Endothelial and Progenitor Cells As Biomarkers for Antiangiogenic Therapy
J. Clin. Oncol.,
March 20, 2006;
24(9):
1449 - 1453.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Bazzoni and E. Dejana
Endothelial Cell-to-Cell Junctions: Molecular Organization and Role in Vascular Homeostasis
Physiol Rev,
July 1, 2004;
84(3):
869 - 901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J Makin, A. D Blann, N. A.Y Chung, S. H Silverman, and G. Y.H Lip
Assessment of endothelial damage in atherosclerotic vascular disease by quantification of circulating endothelial cells: Relationship with von Willebrand factor and tissue factor
Eur. Heart J.,
March 1, 2004;
25(5):
371 - 376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. S. Kaufman, R. L. Lewis, E. T. Hanson, R. Auerbach, J. Plendl, and J. A. Thomson
Functional endothelial cells derived from rhesus monkey embryonic stem cells
Blood,
February 15, 2004;
103(4):
1325 - 1332.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. V. Beerepoot, N. Mehra, J. S. P. Vermaat, B. A. Zonnenberg, M. F. G. B. Gebbink, and E. E. Voest
Increased levels of viable circulating endothelial cells are an indicator of progressive disease in cancer patients
Ann. Onc.,
January 1, 2004;
15(1):
139 - 145.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Ishida, R. K. Kundu, E. Yang, K.-i. Hirata, Y.-D. Ho, and T. Quertermous
Targeted Disruption of Endothelial Cell-selective Adhesion Molecule Inhibits Angiogenic Processes in Vitro and in Vivo
J. Biol. Chem.,
September 5, 2003;
278(36):
34598 - 34604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Schulz, V. Petrig, K. Wolf, K. Kratzel, M. Kohler, B. Becker, and M. Pfeifer
Upregulation of MCAM in primary bronchial epithelial cells from patients with COPD
Eur. Respir. J.,
September 1, 2003;
22(3):
450 - 456.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Yan, Y. Lin, D. Yang, Y. Shen, M. Yuan, Z. Zhang, P. Li, H. Xia, L. Li, D. Luo, et al.
A novel anti-CD146 monoclonal antibody, AA98, inhibits angiogenesis and tumor growth
Blood,
July 1, 2003;
102(1):
184 - 191.
[Abstract]
[Full Text]
[PDF]
|
|
|
| |
