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Blood, 15 December 2001, Vol. 98, No. 13, pp. 3849-3852

BRIEF REPORT

Identification of LIL-STAT in monocytic leukemia cells and monocytes after stimulation with interleukin-6 or interferon gamma

Henny H. Lemmink, Leonore Tuyt, Gerlinde Knol, Ellen Krikke, and Edo Vellenga

From the Division of Hematology, Department of Internal Medicine, Academic Hospital Groningen, The Netherlands.

In acute myelogenous leukemia (AML) and adult T-cell leukemia, it has been demonstrated that the transcription factor LIL-STAT is constitutively activated. To identify and characterize this unknown LIL-STAT protein, electrophoretic mobility shift assay (EMSA) and oligoprecipitation assays were performed by using lipopolysaccharide/interleukin-1 (IL-1)-responsive element (LILRE) oligonucleotide probes. EMSA demonstrated a significant increase in LIL-STAT binding to the LILRE oligonucleotides after interferon gamma  (IFN-gamma ) and IL-6 stimulation of THP-1 cells. In unstimulated THP-1 and AML cells, LILRE oligonucleotide probes bound only to STAT1 alpha  and beta  isoforms. The LILRE element showed a significant increase in binding of both alpha  and beta  isoforms of STAT1 and STAT3 upon IFN-gamma and IL-6 stimulation. Similar results were observed with human monocytes upon IL-6 or IFN-gamma stimulation. These studies indicate that LIL-STAT consists of STAT1 and STAT3 proteins that bind to the LILRE DNA consensus site in a stimulus-dependent way.

© 2001 by The American Society of Hematology.
 

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