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Related Collections Hematopoiesis and Stem Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2001, Vol. 98, No. 3, pp. 627-635 HEMATOPOIESIS Fetal liver myelopoiesis occurs through distinct, prospectively isolatable progenitor subsets David Traver, Toshihiro Miyamoto, Julie Christensen, Junko Iwasaki-Arai, Koichi Akashi, and Irving L. Weissman From the Departments of Pathology and Developmental Biology, Stanford University School of Medicine, CA; and the Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Boston, MA. Hematopoietic fate maps in the developing mouse embryo remain imprecise. Definitive, adult-type hematopoiesis first appears in the fetal liver, then progresses to the spleen and bone marrow. Clonogenic common lymphoid progenitors and clonogenic common myeloid progenitors (CMPs) in adult mouse bone marrow that give rise to all lymphoid and myeloid lineages, respectively, have recently been identified. Here it is shown that myelopoiesis in the fetal liver similarly proceeds through a CMP equivalent. Fetal liver CMPs give rise to megakaryocyte-erythrocyte-restricted progenitors (MEPs) and granulocyte-monocyte-restricted progenitors (GMPs) that can also be prospectively isolated by cell surface phenotype. MEPs and GMPs generate mutually exclusive cell types in clonogenic colony assays and in transplantation experiments, suggesting that the lineage restriction observed within each progenitor subset is absolute under normal conditions. Purified progenitor populations were used to analyze expression profiles of various hematopoiesis-related genes. Expression patterns closely matched those of the adult counterpart populations. These results suggest that adult hematopoietic hierarchies are determined early in the development of the definitive immune system and suggest that the molecular mechanisms underlying cell fate decisions within the myeloerythroid lineages are conserved from embryo to adult. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Xu, S. Eleswarapu, H. Geiger, K. Szczur, D. Daria, Y. Zheng, J. Settleman, E. F. Srour, D. A. Williams, and M.-D. Filippi Loss of the Rho GTPase activating protein p190-B enhances hematopoietic stem cell engraftment potential Blood, October 22, 2009; 114(17): 3557 - 3566. [Abstract] [Full Text] [PDF] H. Jin, R. Sood, J. Xu, F. Zhen, M. A. English, P. P. Liu, and Z. Wen Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI Development, February 15, 2009; 136(4): 647 - 654. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020