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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by de Jong, K. Articles by Kuypers, F. A. Search for Related Content PubMed PubMed Citation Articles by de Jong, K. Articles by Kuypers, F. A. Related Collections Red Cells Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 1 August 2001, Vol. 98, No. 3, pp. 860-867 RED CELLS Characterization of the phosphatidylserine-exposing subpopulation of sickle cells Kitty de Jong, Sandra K. Larkin, Lori A. Styles, Robert M. Bookchin, and Frans A. Kuypers From Children's Hospital Oakland Research Institute, Oakland, CA; and Department of Medicine, Albert Einstein College of Medicine, Bronx, NY. Phosphatidylserine (PS), exclusively present in the inner monolayer of the normal red blood cell (RBC) membrane, is exposed in subpopulations of sickle cells. PS-exposing RBCs were found predominantly among the densest and the very light sickle cells. Within the light RBC fraction, PS exposure was found on reticulocytes, transferrin receptor-expressing reticulocytes, and mature RBCs. The last subset contained low-density valinomycin-resistant RBCs, previously shown to have high Na+ and low K+ content. This subpopulation contained the highest percentage of PS-exposing cells. The PS-exposing sickle cells did not show the sustained high cytosolic Ca++ levels that have been shown to activate scramblase activity. Data from this study indicate that PS exposure can occur at different stages in the life of the sickle RBC and that it correlates with the loss of aminophospholipid translocase activity, the only common denominator of the PS-exposing cells. The additional requirement of scramblase activation may occur during transient increases in cytosolic Ca++. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. N. Y. Setty and S. G. Betal Microvascular endothelial cells express a phosphatidylserine receptor: a functionally active receptor for phosphatidylserine-positive erythrocytes Blood, January 15, 2008; 111(2): 905 - 914. [Abstract] [Full Text] [PDF] P. Guchhait, S. K Dasgupta, A. Le, S. Yellapragada, J. A. Lopez, and P. 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	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020