|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2001, Vol. 98, No. 4, pp. 1028-1037
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Stromal cells expressing ephrin-B2 promote the growth and
sprouting of ephrin-B2+ endothelial cells
Xiu-Qin Zhang,
Nobuyuki Takakura,
Yuichi Oike,
Tomohisa Inada,
Nicholas W. Gale,
George D. Yancopoulos, and
Toshio Suda
From the Department of Cell Differentiation, Institute
of Molecular Embryology and Genetics, Kumamoto University, Japan; and
Regeneron Pharmaceuticals, Tarrytown, NY.
Ephrin-B2 is a transmembrane ligand that is specifically expressed
on arterial endothelial cells (ECs) and surrounding cells and interacts
with multiple EphB class receptors. Conversely, EphB4, a specific
receptor for ephrin-B2, is expressed on venous ECs, and both ephrin-B2
and EphB4 play essential roles in vascular development. The
bidirectional signals between EphB4 and ephrin-B2 are thought to be
specific for the interaction between arteries and veins and to regulate
cell mixing and the making of particular boundaries. However, the
molecular mechanism during vasculogenesis and angiogenesis remains
unclear. Manipulative functional studies were performed on these
proteins in an endothelial cell system. Using in vitro stromal cells
(OP9 cells) and a paraaortic splanchnopleura (P-Sp) coculture system,
these studies found that the stromal cells expressing ephrin-B2
promoted vascular network formation and ephrin-B2+ EC
proliferation and that they also induced the recruitment and proliferation of  -smooth muscle actin (-SMA)-positive
cells. Stromal cells expressing EphB4 inhibited vascular network
formation, ephrin-B2+ EC proliferation, and
-SMA+ cell recruitment and proliferation. Thus, these
data suggest that ephrin-B2 and EphB4 mediate reciprocal interactions
between arterial and venous ECs and surrounding cells to form each
characteristic vessel.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
M. A. Schwarz, L. Caldwell, D. Cafasso, and H. Zheng
Emerging pulmonary vasculature lacks fate specification
Am J Physiol Lung Cell Mol Physiol,
January 1, 2009;
296(1):
L71 - L81.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Zhang, H. Dong, B. Wang, S. Zhu, and B. A. Croy
Dynamic Changes Occur in Patterns of Endometrial EFNB2/EPHB4 Expression During the Period of Spiral Arterial Modification in Mice
Biol Reprod,
September 1, 2008;
79(3):
450 - 458.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Schenke-Layland, K. E. Rhodes, E. Angelis, Y. Butylkova, S. Heydarkhan-Hagvall, C. Gekas, R. Zhang, J. I. Goldhaber, H. K. Mikkola, K. Plath, et al.
Reprogrammed Mouse Fibroblasts Differentiate into Cells of the Cardiovascular and Hematopoietic Lineages
Stem Cells,
June 1, 2008;
26(6):
1537 - 1546.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Huang, Y. Yamada, H. Kidoya, H. Naito, Y. Nagahama, L. Kong, S.-Y. Katoh, W.-l. Li, M. Ueno, and N. Takakura
EphB4 Overexpression in B16 Melanoma Cells Affects Arterial-Venous Patterning in Tumor Angiogenesis
Cancer Res.,
October 15, 2007;
67(20):
9800 - 9808.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Schmidt, K. Brixius, and W. Bloch
Endothelial Precursor Cell Migration During Vasculogenesis
Circ. Res.,
July 20, 2007;
101(2):
125 - 136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Red-Horse, M. Kapidzic, Y. Zhou, K.-T. Feng, H. Singh, and S. J. Fisher
EPHB4 regulates chemokine-evoked trophoblast responses: a mechanism for incorporating the human placenta into the maternal circulation
Development,
September 15, 2005;
132(18):
4097 - 4106.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. O. Zamora, M. H. Davies, S. R. Planck, J. T. Rosenbaum, and M. R. Powers
Soluble Forms of EphrinB2 and EphB4 Reduce Retinal Neovascularization in a Model of Proliferative Retinopathy
Invest. Ophthalmol. Vis. Sci.,
June 1, 2005;
46(6):
2175 - 2182.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-i. Hayashi, T. Asahara, H. Masuda, J. M. Isner, and D. W. Losordo
Functional Ephrin-B2 Expression for Promotive Interaction Between Arterial and Venous Vessels in Postnatal Neovascularization
Circulation,
May 3, 2005;
111(17):
2210 - 2218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Eichmann, T. Makinen, and K. Alitalo
Neural guidance molecules regulate vascular remodeling and vessel navigation
Genes & Dev.,
May 1, 2005;
19(9):
1013 - 1021.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Yuan, T.-M. Hong, J. J. W. Chen, W. H. Tsai, and M. T. Lin
Syndecan-1 up-regulated by ephrinB2/EphB4 plays dual roles in inflammatory angiogenesis
Blood,
August 15, 2004;
104(4):
1025 - 1033.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Tsunematsu, K. Nakayama, Y. Oike, M. Nishiyama, N. Ishida, S. Hatakeyama, Y. Bessho, R. Kageyama, T. Suda, and K. I. Nakayama
Mouse Fbw7/Sel-10/Cdc4 Is Required for Notch Degradation during Vascular Development
J. Biol. Chem.,
March 5, 2004;
279(10):
9417 - 9423.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Fuller, T. Korff, A. Kilian, G. Dandekar, and H. G. Augustin
Forward EphB4 signaling in endothelial cells controls cellular repulsion and segregation from ephrinB2 positive cells
J. Cell Sci.,
June 15, 2003;
116(12):
2461 - 2470.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Hamada, Y. Oike, Y. Ito, H. Maekawa, K. Miyata, T. Shimomura, and T. Suda
Distinct Roles of Ephrin-B2 Forward and EphB4 Reverse Signaling in Endothelial Cells
Arterioscler. Thromb. Vasc. Biol.,
February 1, 2003;
23(2):
190 - 197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Oike, Y. Ito, K. Hamada, X.-Q. Zhang, K. Miyata, F. Arai, T. Inada, K. Araki, N. Nakagata, M. Takeya, et al.
Regulation of vasculogenesis and angiogenesis by EphB/ephrin-B2 signaling between endothelial cells and surrounding mesenchymal cells
Blood,
July 30, 2002;
100(4):
1326 - 1333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
