|
|
 Previous Article | Table of Contents | Next Article 
Blood, 15 August 2001, Vol. 98, No. 4, pp. 1038-1046
HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY
Platelet glycoprotein V binds to collagen and participates in
platelet adhesion and aggregation
Sylvie Moog,
Pierre Mangin,
Nadège Lenain,
Catherine Strassel,
Catherine Ravanat,
Simone Schuhler,
Monique Freund,
Martine Santer,
Mark Kahn,
Bernhard Nieswandt,
Christian Gachet,
Jean-Pierre Cazenave, and
François Lanza
From INSERM U.311, Etablissement Français du
Sang-Alsace, Strasbourg, France; University of Pennsylvania,
Philadelphia, PA; and Witten/Herdecke University, Klinikum Wuppertal,
Germany.
Glycoprotein V (GPV) is a subunit of the platelet GPIb-V-IX
receptor for von Willebrand factor and thrombin. GPV is cleaved from
the platelet surface during activation by thrombin, but its role in
hemostasis is still unknown. It is reported that GPV knockout mice had
a decreased tendency to form arterial occluding thrombi in an
intravital thrombosis model and abnormal platelet interaction with the
subendothelium. In vitro, GPV-deficient platelets exhibited defective
adhesion to a collagen type I-coated surface under flow or static
conditions. Aggregation studies demonstrated a decreased response of
the GPV-deficient platelets to collagen, reflected by an increased lag
phase and reduced amplitude of aggregation. Responses to adenosine
diphosphate, arachidonic acid, and the thromboxane analog U46619 were
normal but were enhanced to low thrombin concentrations. The defect of
GPV null platelets made them more sensitive to inhibition by the
anti-GPVI monoclonal antibody (mAb) JAQ1, and this was also the case in
aspirin- or apyrase-treated platelets. Moreover, an mAb (V.3) against
the extracellular domain of human GPV selectively inhibited
collagen-induced aggregation in human or rat platelets. V.3
injected in rats as a bolus decreased the ex vivo collagen aggregation
response without affecting the platelet count. Finally, surface plasmon
resonance studies demonstrated binding of recombinant soluble GPV on a
collagen-coupled matrix. In conclusion, GPV binds to collagen and
appears to be required for normal platelet responses to this agonist.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
R. K. Andrews, D. Karunakaran, E. E. Gardiner, and M. C. Berndt
Platelet Receptor Proteolysis: A Mechanism for Downregulating Platelet Reactivity
Arterioscler. Thromb. Vasc. Biol.,
July 1, 2007;
27(7):
1511 - 1520.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
U. J.H. Sachs and B. Nieswandt
In Vivo Thrombus Formation in Murine Models
Circ. Res.,
April 13, 2007;
100(7):
979 - 991.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Ajzenberg, C. Berroeta, I. Philip, B. Grandchamp, P. Ducellier, V. Huart, P. Verpillat, M.-C. Guillin, and J. Benessiano
Association of the -92C/G and 807C/T Polymorphisms of the {alpha}2 Subunit Gene With Human Platelets {alpha}2{beta}1 Receptor Density
Arterioscler. Thromb. Vasc. Biol.,
August 1, 2005;
25(8):
1756 - 1760.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. F.W. Keuren, S. J.H. Wielders, H. Ulrichts, T. Hackeng, J. W.M. Heemskerk, H. Deckmyn, E. M. Bevers, and T. Lindhout
Synergistic Effect of Thrombin on Collagen-Induced Platelet Procoagulant Activity Is Mediated Through Protease-Activated Receptor-1
Arterioscler. Thromb. Vasc. Biol.,
July 1, 2005;
25(7):
1499 - 1505.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Rabie, A. Strehl, A. Ludwig, and B. Nieswandt
Evidence for a Role of ADAM17 (TACE) in the Regulation of Platelet Glycoprotein V
J. Biol. Chem.,
April 15, 2005;
280(15):
14462 - 14468.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Goncalves, W. S. Nesbitt, Y. Yuan, and S. P. Jackson
Importance of Temporal Flow Gradients and Integrin {alpha}IIb{beta}3 Mechanotransduction for Shear Activation of Platelets
J. Biol. Chem.,
April 15, 2005;
280(15):
15430 - 15437.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. R.-M. Siljander, I. C. A. Munnix, P. A. Smethurst, H. Deckmyn, T. Lindhout, W. H. Ouwehand, R. W. Farndale, and J. W. M. Heemskerk
Platelet receptor interplay regulates collagen-induced thrombus formation in flowing human blood
Blood,
February 15, 2004;
103(4):
1333 - 1341.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X.-H. Leng, S. Y. Hong, S. Larrucea, W. Zhang, T.-T. Li, J. A. Lopez, and P. F. Bray
Platelets of Female Mice Are Intrinsically More Sensitive to Agonists Than Are Platelets of Males
Arterioscler. Thromb. Vasc. Biol.,
February 1, 2004;
24(2):
376 - 381.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. Best, Y. A. Senis, G. E. Jarvis, H. J. Eagleton, D. J. Roberts, T. Saito, S. M. Jung, M. Moroi, P. Harrison, F. R. Green, et al.
GPVI levels in platelets: relationship to platelet function at high shear
Blood,
October 15, 2003;
102(8):
2811 - 2818.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Goncalves, S. C. Hughan, S. M. Schoenwaelder, C. L. Yap, Y. Yuan, and S. P. Jackson
Integrin {alpha}IIb{beta}3-dependent Calcium Signals Regulate Platelet-Fibrinogen Interactions under Flow: INVOLVEMENT OF PHOSPHOLIPASE C{gamma}2
J. Biol. Chem.,
September 12, 2003;
278(37):
34812 - 34822.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Mangin, Y. Yuan, I. Goncalves, A. Eckly, M. Freund, J.-P. Cazenave, C. Gachet, S. P. Jackson, and F. Lanza
Signaling Role for Phospholipase C{gamma}2 in Platelet Glycoprotein Ib{alpha} Calcium Flux and Cytoskeletal Reorganization: INVOLVEMENT OF A PATHWAY DISTINCT FROM FcR{gamma} CHAIN AND Fc{gamma}RIIA
J. Biol. Chem.,
August 29, 2003;
278(35):
32880 - 32891.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Nieswandt and S. P. Watson
Platelet-collagen interaction: is GPVI the central receptor?
Blood,
July 15, 2003;
102(2):
449 - 461.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Lagadec, O. Dejoux, M. Ticchioni, F. Cottrez, M. Johansen, E. J. Brown, and A. Bernard
Involvement of a CD47-dependent pathway in platelet adhesion on inflamed vascular endothelium under flow
Blood,
June 15, 2003;
101(12):
4836 - 4843.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. K. Larson, H. Chen, M. L. Kahn, A. M. Taylor, J.-E. Fabre, R. M. Mortensen, P. B. Conley, and L. V. Parise
Identification of P2Y12-dependent and -independent mechanisms of glycoprotein VI-mediated Rap1 activation in platelets
Blood,
February 15, 2003;
101(4):
1409 - 1415.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Patel, H. Vaananen, M. Jirouskova, T. Hoffmann, C. Bodian, and B. S. Coller
Dynamics of GPIIb/IIIa-mediated platelet-platelet interactions in platelet adhesion/thrombus formation on collagen in vitro as revealed by videomicroscopy
Blood,
February 1, 2003;
101(3):
929 - 936.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Massberg, M. Gawaz, S. Gruner, V. Schulte, I. Konrad, D. Zohlnhofer, U. Heinzmann, and B. Nieswandt
A Crucial Role of Glycoprotein VI for Platelet Recruitment to the Injured Arterial Wall In Vivo
J. Exp. Med.,
January 6, 2003;
197(1):
41 - 49.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Leo, J. Di Paola, B. A. Judd, G. A. Koretzky, and S. R. Lentz
Role of the adapter protein SLP-76 in GPVI-dependent platelet procoagulant responses to collagen
Blood,
September 26, 2002;
100(8):
2839 - 2844.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. F. Garner, K. Campbell, P. Metcalfe, J. Keidan, E. Huiskes, J.-F. Dong, J. A. Lopez, and W. H. Ouwehand
Glycoprotein V: the predominant target antigen in gold-induced autoimmune thrombocytopenia
Blood,
June 17, 2002;
100(1):
344 - 346.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Holtkotter, B. Nieswandt, N. Smyth, W. Muller, M. Hafner, V. Schulte, T. Krieg, and B. Eckes
Integrin alpha 2-Deficient Mice Develop Normally, Are Fertile, but Display Partially Defective Platelet Interaction with Collagen
J. Biol. Chem.,
March 22, 2002;
277(13):
10789 - 10794.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. J. Kunicki
The Influence of Platelet Collagen Receptor Polymorphisms in Hemostasis and Thrombotic Disease
Arterioscler. Thromb. Vasc. Biol.,
January 1, 2002;
22(1):
14 - 20.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
