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This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hochberg, E. P. Articles by Ritz, J. Search for Related Content PubMed PubMed Citation Articles by Hochberg, E. P. Articles by Ritz, J. Related Collections Immunobiology Transplantation Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2001, Vol. 98, No. 4, pp. 1116-1121 IMMUNOBIOLOGY Quantitation of T-cell neogenesis in vivo after allogeneic bone marrow transplantation in adults Ephraim P. Hochberg, Antoinette C. Chillemi, Catherine J. Wu, Donna Neuberg, Christine Canning, Kelly Hartman, Edwin P. Alyea, Robert J. Soiffer, Spyros A. Kalams, and Jerome Ritz From the Disease Center for Hematologic Oncology, Department of Adult Oncology, Department of Biostatistics, Dana-Farber Cancer Institute; Department of Medicine, Brigham and Women's Hospital; Partners AIDS Research Center, Massachusetts General Hospital; and Harvard Medical School, Boston MA. Following myeloablative therapy, it is unknown to what extent age-dependent thymic involution limits the generation of new T cells with a diverse repertoire. Normal T-cell receptor gene rearrangement in T-cell progenitors results in the generation of T-cell receptor rearrangement excision circles (TRECs). In this study, a quantitative assay for TRECs was used to measure T-cell neogenesis in adult patients with leukemia who received myeloablative therapy followed by transplantation of allogeneic hematopoietic stem cells. Although phenotypically mature T cells had recovered by 1 to 2 months after bone marrow transplantation (BMT), TREC levels remained low for 3 months after BMT. T-cell neogenesis became evident by 6 months, and normal levels of adult thymic function were restored at 6 to 12 months after BMT. Subsequent leukemia relapse in some patients was associated with reduced TREC levels, but infusion of mature donor CD4+ T cells resulted in rapid restoration of thymic function. These studies demonstrate that T-cell neogenesis contributes to immune reconstitution in adult patients and suggest that thymic function can be manipulated in vivo. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. Clave, M. Busson, C. Douay, R. Peffault de Latour, J. Berrou, C. Rabian, M. Carmagnat, V. Rocha, D. Charron, G. Socie, et al. 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