SEARCH:   Advanced

This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Burns, S. Articles by Jones, G. E. Search for Related Content PubMed PubMed Citation Articles by Burns, S. Articles by Jones, G. E. Related Collections Immunobiology Cell Adhesion and Motility Signal Transduction Social Bookmarking                   What's this?  Previous Article  |  Table of Contents  |  Next Article  Blood, 15 August 2001, Vol. 98, No. 4, pp. 1142-1149 IMMUNOBIOLOGY Configuration of human dendritic cell cytoskeleton by Rho GTPases, the WAS protein, and differentiation Siobhan Burns, Adrian J. Thrasher, Michael P. Blundell, Laura Machesky, and Gareth E. Jones From the Molecular Immunology Unit, Institute of Child Health, University College London; Great Ormond Street Hospital for Children NHS Trust, London; Department of Biochemistry, University of Birmingham; and Randall Centre for Molecular Mechanisms of Cell Function, Kings College London, United Kingdom. The cellular mechanisms that configure the cytoskeleton during migration of dendritic cells (DCs) are poorly understood. Immature DCs assemble specialized adhesion structures known as podosomes at their leading edge; these are associated with the localized recruitment of the Wiskott-Aldrich Syndrome protein (WASp) and the actin organizing actin-related protein 2/3 complex. In immature DCs lacking WASp, podosomes are absent, residual dysmorphic lamellipodia and filopodia are nonpolarized, and migration is severely compromised. Microinjection studies indicate that podosome assembly and polarization require concerted action of Cdc42, Rac, and Rho, thereby providing a link between sequential protrusive and adhesive activity. Formation of podosomes is restricted to cells with an immature phenotype, indicating a specific role for these structures during the early migratory phase. © 2001 by The American Society of Hematology.   CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Dovas, J.-C. Gevrey, A. Grossi, H. Park, W. Abou-Kheir, and D. Cox Regulation of podosome dynamics by WASp phosphorylation: implication in matrix degradation and chemotaxis in macrophages J. Cell Sci., November 1, 2009; 122(21): 3873 - 3882. [Abstract] [Full Text] [PDF] M. P. Blundell, G. Bouma, J. Metelo, A. Worth, Y. Calle, L. A. Cowell, L. S. Westerberg, D. A. Moulding, S. Mirando, C. Kinnon, et al. Phosphorylation of WASp is a key regulator of activity and stability in vivo PNAS, September 15, 2009; 106(37): 15738 - 15743. [Abstract] [Full Text] [PDF] S. Linder Invadosomes at a glance J. Cell Sci., September 1, 2009; 122(17): 3009 - 3013. [Full Text] [PDF] C. Albiges-Rizo, O. Destaing, B. Fourcade, E. Planus, and M. R. Block Actin machinery and mechanosensitivity in invadopodia, podosomes and focal adhesions J. Cell Sci., September 1, 2009; 122(17): 3037 - 3049. [Abstract] [Full Text] [PDF] D. R. Spurrell, N. A. Luckashenak, D. C. Minney, A. Chaplin, J. M. Penninger, R. S. Liwski, J. L. Clements, and K. A. West Vav1 Regulates the Migration and Adhesion of Dendritic Cells J. Immunol., July 1, 2009; 183(1): 310 - 318. [Abstract] [Full Text] [PDF] M. Bosticardo, F. Marangoni, A. Aiuti, A. Villa, and M. Grazia Roncarolo Recent advances in understanding the pathophysiology of Wiskott-Aldrich syndrome Blood, June 18, 2009; 113(25): 6288 - 6295. [Abstract] [Full Text] [PDF] T. Lammermann, J. Renkawitz, X. Wu, K. Hirsch, C. Brakebusch, and M. Sixt Cdc42-dependent leading edge coordination is essential for interstitial dendritic cell migration Blood, June 4, 2009; 113(23): 5703 - 5710. [Abstract] [Full Text] [PDF] C. Ocana-Morgner, C. Wahren, and R. Jessberger SWAP-70 regulates RhoA/RhoB-dependent MHCII surface localization in dendritic cells Blood, February 12, 2009; 113(7): 1474 - 1482. [Abstract] [Full Text] [PDF] Q. He, F. O. Eko, D. Lyn, G. A. Ananaba, C. Bandea, J. Martinez, K. Joseph, K. Kellar, C. M. Black, and J. U. Igietseme Involvement of LEK1 in Dendritic Cell Regulation of T Cell Immunity against Chlamydia J. Immunol., September 15, 2008; 181(6): 4037 - 4042. [Abstract] [Full Text] [PDF] M. A. West, A. R. Prescott, K. M. Chan, Z. Zhou, S. Rose-John, J. Scheller, and C. Watts TLR ligand-induced podosome disassembly in dendritic cells is ADAM17 dependent J. Cell Biol., September 8, 2008; 182(5): 993 - 1005. [Abstract] [Full Text] [PDF] J. Pulecio, E. Tagliani, A. Scholer, F. Prete, L. Fetler, O. R. Burrone, and F. Benvenuti Expression of Wiskott-Aldrich Syndrome Protein in Dendritic Cells Regulates Synapse Formation and Activation of Naive CD8+ T Cells J. Immunol., July 15, 2008; 181(2): 1135 - 1142. [Abstract] [Full Text] [PDF] A. Dorfleutner, Y. Cho, D. Vincent, J. Cunnick, H. Lin, S. A. Weed, C. Stehlik, and D. C. Flynn Phosphorylation of AFAP-110 affects podosome lifespan in A7r5 cells J. Cell Sci., July 15, 2008; 121(14): 2394 - 2405. [Abstract] [Full Text] [PDF] S. F. G. van Helden, M. M. Oud, B. Joosten, N. Peterse, C. G. Figdor, and F. N. van Leeuwen PGE2-mediated podosome loss in dendritic cells is dependent on actomyosin contraction downstream of the RhoA-Rho-kinase axis J. Cell Sci., April 1, 2008; 121(7): 1096 - 1106. [Abstract] [Full Text] [PDF] C. Nobile, M. Lind, F. Miro, K. Chemin, M. Tourret, G. Occhipinti, S. Dogniaux, S. Amigorena, and C. Hivroz Cognate CD4+ T-cell-dendritic cell interactions induce migration of immature dendritic cells through dissolution of their podosomes Blood, April 1, 2008; 111(7): 3579 - 3590. [Abstract] [Full Text] [PDF] G. Bouma, S. Burns, and A. J. Thrasher Impaired T-cell priming in vivo resulting from dysfunction of WASp-deficient dendritic cells Blood, December 15, 2007; 110(13): 4278 - 4284. [Abstract] [Full Text] [PDF] A. Chabadel, I. Banon-Rodriguez, D. Cluet, B. B. Rudkin, B. Wehrle-Haller, E. Genot, P. Jurdic, I. M. Anton, and F. Saltel CD44 and beta3 Integrin Organize Two Functionally Distinct Actin-based Domains in Osteoclasts Mol. Biol. Cell, December 1, 2007; 18(12): 4899 - 4910. [Abstract] [Full Text] [PDF] M. Kato, S. Khan, E. d'Aniello, K. J. McDonald, and D. N. J. Hart The Novel Endocytic and Phagocytic C-Type Lectin Receptor DCL-1/CD302 on Macrophages Is Colocalized with F-Actin, Suggesting a Role in Cell Adhesion and Migration J. Immunol., November 1, 2007; 179(9): 6052 - 6063. [Abstract] [Full Text] [PDF] J. M. Vyas, Y.-M. Kim, K. Artavanis-Tsakonas, J. C. Love, A. G. Van der Veen, and H. L. Ploegh Tubulation of Class II MHC Compartments Is Microtubule Dependent and Involves Multiple Endolysosomal Membrane Proteins in Primary Dendritic Cells J. Immunol., June 1, 2007; 178(11): 7199 - 7210. [Abstract] [Full Text] [PDF] M. S. Arredouani, F. Franco, A. Imrich, A. Fedulov, X. Lu, D. Perkins, R. Soininen, K. Tryggvason, S. D. Shapiro, and L. Kobzik Scavenger Receptors SR-AI/II and MARCO Limit Pulmonary Dendritic Cell Migration and Allergic Airway Inflammation J. Immunol., May 1, 2007; 178(9): 5912 - 5920. [Abstract] [Full Text] [PDF] T. Morita, T. Mayanagi, T. Yoshio, and K. Sobue Changes in the Balance between Caldesmon Regulated by p21-activated Kinases and the Arp2/3 Complex Govern Podosome Formation J. Biol. Chem., March 16, 2007; 282(11): 8454 - 8463. [Abstract] [Full Text] [PDF] S. Tsuboi Requirement for a Complex of Wiskott-Aldrich Syndrome Protein (WASP) with WASP Interacting Protein in Podosome Formation in Macrophages J. Immunol., March 1, 2007; 178(5): 2987 - 2995. [Abstract] [Full Text] [PDF] N. A. Luckashenak, R. L. Ryszkiewicz, K. D. Ramsey, and J. L. Clements The Src Homology 2 Domain-Containing Leukocyte Protein of 76-kDa Adaptor Links Integrin Ligation with p44/42 MAPK Phosphorylation and Podosome Distribution in Murine Dendritic Cells J. Immunol., October 15, 2006; 177(8): 5177 - 5185. [Abstract] [Full Text] [PDF] P. J. Ancliff, M. P. Blundell, G. O. Cory, Y. Calle, A. Worth, H. Kempski, S. Burns, G. E. Jones, J. Sinclair, C. Kinnon, et al. Two novel activating mutations in the Wiskott-Aldrich syndrome protein result in congenital neutropenia Blood, October 1, 2006; 108(7): 2182 - 2189. [Abstract] [Full Text] [PDF] S. F. G. van Helden, D. J. E. B. Krooshoop, K. C. M. Broers, R. A. P. Raymakers, C. G. Figdor, and F. N. van Leeuwen A Critical Role for Prostaglandin E2 in Podosome Dissolution and Induction of High-Speed Migration during Dendritic Cell Maturation J. Immunol., August 1, 2006; 177(3): 1567 - 1574. [Abstract] [Full Text] [PDF] A. P. Wheeler, C. M. Wells, S. D. Smith, F. M. Vega, R. B. Henderson, V. L. Tybulewicz, and A. J. Ridley Rac1 and Rac2 regulate macrophage morphology but are not essential for migration J. Cell Sci., July 1, 2006; 119(13): 2749 - 2757. [Abstract] [Full Text] [PDF] S. Tehrani, R. Faccio, I. Chandrasekar, F. P. Ross, and J. A. Cooper Cortactin Has an Essential and Specific Role in Osteoclast Actin Assembly Mol. Biol. Cell, July 1, 2006; 17(7): 2882 - 2895. [Abstract] [Full Text] [PDF] Y. Calle, N. O. Carragher, A. J. Thrasher, and G. E. Jones Inhibition of calpain stabilises podosomes and impairs dendritic cell motility J. Cell Sci., June 1, 2006; 119(11): 2375 - 2385. [Abstract] [Full Text] [PDF] P. Kopp, R. Lammers, M. Aepfelbacher, G. Woehlke, T. Rudel, N. Machuy, W. Steffen, and S. Linder The Kinesin KIF1C and Microtubule Plus Ends Regulate Podosome Dynamics in Macrophages Mol. Biol. Cell, June 1, 2006; 17(6): 2811 - 2823. [Abstract] [Full Text] [PDF] C. Varon, F. Tatin, V. Moreau, E. Van Obberghen-Schilling, S. Fernandez-Sauze, E. Reuzeau, I. Kramer, and E. Genot Transforming Growth Factor {beta} Induces Rosettes of Podosomes in Primary Aortic Endothelial Cells. Mol. Cell. Biol., May 1, 2006; 26(9): 3582 - 3594. [Abstract] [Full Text] [PDF] R. Eves, B. A. Webb, S. Zhou, and A. S. Mak Caldesmon is an integral component of podosomes in smooth muscle cells J. Cell Sci., May 1, 2006; 119(9): 1691 - 1702. [Abstract] [Full Text] [PDF] W. Cao, P. Tan, C. H. Lee, H. Zhang, and J. Lu A transforming growth factor-beta-induced protein stimulates endocytosis and is up-regulated in immature dendritic cells Blood, April 1, 2006; 107(7): 2777 - 2785. [Abstract] [Full Text] [PDF] S. Zhou, B. A. Webb, R. Eves, and A. S. Mak Effects of tyrosine phosphorylation of cortactin on podosome formation in A7r5 vascular smooth muscle cells Am J Physiol Cell Physiol, February 1, 2006; 290(2): C463 - C471. [Abstract] [Full Text] [PDF] B. A. Webb, R. Eves, S. W. Crawley, S. Zhou, G. P. Cote, and A. S. Mak PAK1 induces podosome formation in A7r5 vascular smooth muscle cells in a PAK-interacting exchange factor-dependent manner Am J Physiol Cell Physiol, October 1, 2005; 289(4): C898 - C907. [Abstract] [Full Text] [PDF] M. A. Chellaiah Regulation of Actin Ring Formation by Rho GTPases in Osteoclasts J. Biol. Chem., September 23, 2005; 280(38): 32930 - 32943. [Abstract] [Full Text] [PDF] N. Czeloth, G. Bernhardt, F. Hofmann, H. Genth, and R. Forster Sphingosine-1-Phosphate Mediates Migration of Mature Dendritic Cells J. Immunol., September 1, 2005; 175(5): 2960 - 2967. [Abstract] [Full Text] [PDF] O. Destaing, F. Saltel, B. Gilquin, A. Chabadel, S. Khochbin, S. Ory, and P. Jurdic A novel Rho-mDia2-HDAC6 pathway controls podosome patterning through microtubule acetylation in osteoclasts J. Cell Sci., July 1, 2005; 118(13): 2901 - 2911. [Abstract] [Full Text] [PDF] S. B. Snapper, P. Meelu, D. Nguyen, B. M. Stockton, P. Bozza, F. W. Alt, F. S. Rosen, U. H. von Andrian, and C. Klein WASP deficiency leads to global defects of directed leukocyte migration in vitro and in vivo J. Leukoc. Biol., June 1, 2005; 77(6): 993 - 998. [Abstract] [Full Text] [PDF] S. Linder and P. Kopp Podosomes at a glance J. Cell Sci., May 15, 2005; 118(10): 2079 - 2082. [Full Text] [PDF] G. V. Shurin, I. L. Tourkova, G. S. Chatta, G. Schmidt, S. Wei, J. Y. Djeu, and M. R. Shurin Small Rho GTPases Regulate Antigen Presentation in Dendritic Cells J. Immunol., March 15, 2005; 174(6): 3394 - 3400. [Abstract] [Full Text] [PDF] S. de Noronha, S. Hardy, J. Sinclair, M. P. Blundell, J. Strid, O. Schulz, J. Zwirner, G. E. Jones, D. R. Katz, C. Kinnon, et al. Impaired dendritic-cell homing in vivo in the absence of Wiskott-Aldrich syndrome protein Blood, February 15, 2005; 105(4): 1590 - 1597. [Abstract] [Full Text] [PDF] L. Westerberg, M. Larsson, S. J. Hardy, C. Fernandez, A. J. Thrasher, and E. Severinson Wiskott-Aldrich syndrome protein deficiency leads to reduced B-cell adhesion, migration, and homing, and a delayed humoral immune response Blood, February 1, 2005; 105(3): 1144 - 1152. [Abstract] [Full Text] [PDF] K. M. Kerksiek, F. Niedergang, P. Chavrier, D. H. Busch, and T. Brocker Selective Rac1 inhibition in dendritic cells diminishes apoptotic cell uptake and cross-presentation in vivo Blood, January 15, 2005; 105(2): 742 - 749. [Abstract] [Full Text] [PDF] R Alikhan, F Peters, R Wilmott, and A T Cohen Fatal pulmonary embolism in hospitalised patients: a necropsy review J. Clin. Pathol., December 1, 2004; 57(12): 1254 - 1257. [Abstract] [Full Text] [PDF] S. Burns, G. O. Cory, W. Vainchenker, and A. J. Thrasher Mechanisms of WASp-mediated hematologic and immunologic disease Blood, December 1, 2004; 104(12): 3454 - 3462. [Abstract] [Full Text] [PDF] M. A. West, R. P. A. Wallin, S. P. Matthews, H. G. Svensson, R. Zaru, H.-G. Ljunggren, A. R. Prescott, and C. Watts Enhanced Dendritic Cell Antigen Capture via Toll-Like Receptor-Induced Actin Remodeling Science, August 20, 2004; 305(5687): 1153 - 1157. [Abstract] [Full Text] [PDF] R. L. Berdeaux, B. Diaz, L. Kim, and G. S. Martin Active Rho is localized to podosomes induced by oncogenic Src and is required for their assembly and function J. Cell Biol., August 2, 2004; 166(3): 317 - 323. [Abstract] [Full Text] [PDF] S. Jaksits, W. Bauer, E. Kriehuber, M. Zeyda, T. M. Stulnig, G. Stingl, E. Fiebiger, and D. Maurer Lipid Raft-Associated GTPase Signaling Controls Morphology and CD8+ T Cell Stimulatory Capacity of Human Dendritic Cells J. Immunol., August 1, 2004; 173(3): 1628 - 1639. [Abstract] [Full Text] [PDF] A. Gismondi, L. Cifaldi, C. Mazza, S. Giliani, S. Parolini, S. Morrone, J. Jacobelli, E. Bandiera, L. Notarangelo, and A. Santoni Impaired natural and CD16-mediated NK cell cytotoxicity in patients with WAS and XLT: ability of IL-2 to correct NK cell functional defect Blood, July 15, 2004; 104(2): 436 - 443. [Abstract] [Full Text] [PDF] R. Badolato Leukocyte circulation: one-way or round-trip? Lessons from primary immunodeficiency patients J. Leukoc. Biol., July 1, 2004; 76(1): 1 - 6. [Abstract] [Full Text] [PDF] Y. Calle, G. E. Jones, C. Jagger, K. Fuller, M. P. Blundell, J. Chow, T. Chambers, and A. J. Thrasher WASp deficiency in mice results in failure to form osteoclast sealing zones and defects in bone resorption Blood, May 1, 2004; 103(9): 3552 - 3561. [Abstract] [Full Text] [PDF] H. Guan, G. Zu, Y. Xie, H. Tang, M. Johnson, X. Xu, C. Kevil, W.-C. Xiong, C. Elmets, Y. Rao, et al. Neuronal Repellent Slit2 Inhibits Dendritic Cell Migration and the Development of Immune Responses J. Immunol., December 15, 2003; 171(12): 6519 - 6526. [Abstract] [Full Text] [PDF] M. G. QUARANTA, B. MATTIOLI, F. SPADARO, E. STRAFACE, L. GIORDANI, C. RAMONI, W. MALORNI, and M. VIORA HIV-1 Nef triggers Vav-mediated signaling pathway leading to functional and morphological differentiation of dendritic cells FASEB J, November 1, 2003; 17(14): 2025 - 2036. [Abstract] [Full Text] [PDF] T. S. Strom, S. J. Turner, S. Andreansky, H. Liu, P. C. Doherty, D. K. Srivastava, J. M. Cunningham, and A. W. Nienhuis Defects in T-cell-mediated immunity to influenza virus in murine Wiskott-Aldrich syndrome are corrected by oncoretroviral vector-mediated gene transfer into repopulating hematopoietic cells Blood, November 1, 2003; 102(9): 3108 - 3116. [Abstract] [Full Text] [PDF] F. Granucci, F. Petralia, M. Urbano, S. Citterio, F. Di Tota, L. Santambrogio, and P. Ricciardi-Castagnoli The scavenger receptor MARCO mediates cytoskeleton rearrangements in dendritic cells and microglia Blood, October 15, 2003; 102(8): 2940 - 2947. [Abstract] [Full Text] [PDF] V. Moreau, F. Tatin, C. Varon, and E. Genot Actin Can Reorganize into Podosomes in Aortic Endothelial Cells, a Process Controlled by Cdc42 and RhoA Mol. Cell. Biol., October 1, 2003; 23(19): 6809 - 6822. [Abstract] [Full Text] [PDF] C. Lacout, E. Haddad, S. Sabri, F. Svinarchouk, L. Garcon, C. Capron, A. Foudi, R. Mzali, S. B. Snapper, F. Louache, et al. A defect in hematopoietic stem cell migration explains the nonrandom X-chromosome inactivation in carriers of Wiskott-Aldrich syndrome Blood, August 15, 2003; 102(4): 1282 - 1289. [Abstract] [Full Text] [PDF] J. R. Brown, D. Goldblatt, J. Buddle, L. Morton, and A. J. Thrasher Diminished production of anti-inflammatory mediators during neutrophil apoptosis and macrophage phagocytosis in chronic granulomatous disease (CGD) J. Leukoc. Biol., May 1, 2003; 73(5): 591 - 599. [Abstract] [Full Text] [PDF] R. Dong, K. Cwynarski, A. Entwistle, F. Marelli-Berg, F. Dazzi, E. Simpson, J. M. Goldman, J. V. Melo, R. I. Lechler, I. Bellantuono, et al. Dendritic cells from CML patients have altered actin organization, reduced antigen processing, and impaired migration Blood, May 1, 2003; 101(9): 3560 - 3567. [Abstract] [Full Text] [PDF] J. Magdalena, T. H. Millard, S. Etienne-Manneville, S. Launay, H. K. Warwick, and L. M. Machesky Involvement of the Arp2/3 Complex and Scar2 in Golgi Polarity in Scratch Wound Models Mol. Biol. Cell, February 1, 2003; 14(2): 670 - 684. [Abstract] [Full Text] [PDF] Y. Samstag, S. M. Eibert, M. Klemke, and G. H. Wabnitz Actin cytoskeletal dynamics in T lymphocyte activation and migration J. Leukoc. Biol., January 1, 2003; 73(1): 30 - 48. [Abstract] [Full Text] [PDF] G. O. C. Cory, R. Garg, R. Cramer, and A. J. Ridley Phosphorylation of Tyrosine 291 Enhances the Ability of WASp to Stimulate Actin Polymerization and Filopodium Formation J. Biol. Chem., November 15, 2002; 277(47): 45115 - 45121. [Abstract] [Full Text] [PDF] H. Falet, K. M. Hoffmeister, R. Neujahr, and J. H. Hartwig Normal Arp2/3 complex activation in platelets lacking WASp Blood, August 28, 2002; 100(6): 2113 - 2122. [Abstract] [Full Text] [PDF]

	Copyright © 2001 by American Society of Hematology         Online ISSN: 1528-0020