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Blood, 15 September 2001, Vol. 98, No. 6, pp. 1852-1857
IMMUNOBIOLOGY
Immunotherapy using heat-shock protein preparations of leukemia
cells after syngeneic bone marrow transplantation in mice
Kazuya Sato,
Yoshihiro Torimoto,
Yasuaki Tamura,
Motohiro Shindo,
Hitoshi Shinzaki,
Katsuyuki Hirai, and
Yutaka Kohgo
From the Third Department of Internal Medicine,
Asahikawa Medical College, Hokkaido, Japan.
Heat-shock proteins (HSPs) act as molecular chaperones binding
endogenous antigenic peptides and transporting them to major histocompatibility complexes. HSPs chaperone a broad repertoire of
endogenous peptides including tumor antigens. For the immunotherapy of
tumors, a strategy using HSPs may be more advantageous than other
procedures because the identification of each tumor-specific antigen is
not necessary. In this study, the efficacy of immunotherapy against
minimal residual leukemia cells using HSP preparations was evaluated.
HSP70 and GP96 were purified from syngeneic leukemia cell line A20 and
immunized into BALB/c mice during the reconstitution period of the
immune system after syngeneic bone marrow transplantation. In this
procedure, all mice not immunized were dead within 60 days of A20
inoculation, whereas the survival times of HSP-immunized mice were
significantly prolonged. In addition, the depletion of either
CD4+ or CD8+ T lymphocyte significantly
abrogated this efficacy, indicating that both CD4+ and
CD8+ T lymphocytes were required for tumor cell rejection.
Moreover, the vaccination of HSPs elicited a specific response of
potent CD8+ T lymphocytes cytotoxic against A20 in vitro.
These observations suggest that immunization of the complex of HSPs and
peptides derived from leukemia cells leads to immune responses. These
immune responses are sufficient to reject minimal amounts of leukemia cells for relatively immunocompromised mice after syngeneic bone marrow transplantation.
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