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Blood, 15 September 2001, Vol. 98, No. 6, pp. 1862-1871

IMMUNOBIOLOGY

Long-lived polyclonal B-cell lines derived from midgestation mouse embryo lymphohematopoietic progenitors reconstitute adult immunodeficient mice

José A. Martínez-M., Susana Minguet, Pilar Gonzalo, Pilar G. Soro, Belén de Andrés, Ana Ízcue, Miguel A. R. Marcos, and María-Luisa Gaspar

From the Centro Nacional de Biología Fundamental, Instituto de Salud Carlos III (ISCIII), Majadahonda 28220, Spain; and the Centro de Biología Molecular S.O. (CBMSO), CSIC-UAM, Campus de Cantoblanco, Madrid, Spain.

Lymphohematopoietic progenitors derived from midgestation mouse embryos were established in long-term cultures with stromal cell monolayers and interleukin 7 (IL-7), giving rise to B-lineage cell lines. The initial emergence and in vitro establishment of these early embryo cell lines were highly sensitive to IL-7-mediated signals, in comparison to cell lines similarly obtained using precursors from late fetal liver (> 13 days postcoitum) and adult bone marrow. The early embryo-derived progenitors spontaneously differentiated in vitro to CD19+IgM+ immature B cells in the presence of optimal concentrations of IL-7, in contrast to those progenitors obtained from late gestation and adult mice, whose differentiation only occurred in the absence of IL-7. The newly in vitro-generated B cells of the early embryo cell lines repopulated adult immunodeficient severe combined immunodeficient mice on their adoptive transfer in vivo and generated specific humoral immune responses after immunization.

© 2001 by The American Society of Hematology.
 

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