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Blood, 1 October 2001, Vol. 98, No. 7, pp. 2275-2278
BRIEF REPORT
Distinct regulatory elements are required for faithful expression
of human CD4 in T cells, macrophages, and dendritic cells of
transgenic mice
Zaher Hanna,
Najet Rebai,
Johanne Poudrier, and
Paul Jolicoeur
From the Laboratory of Molecular Biology, Clinical
Research Institute of Montreal, Montreal, Quebec, Canada; Departments
of Medicine or Microbiology and Immunology, Université de
Montréal, Montreal, Quebec, Canada; and the Division of
Experimental Medicine, McGill University, Montreal, Quebec, Canada.
To identify the regulatory elements controlling expression of the
human CD4 (hCD4) gene in different cell
types of the immune system, deletion and chimeric (human/murine)
reporter genes were constructed and tested in transgenic (Tg) mice.
Regulatory elements required for the proper hCD4 expression in the
immature double-positive thymic T cells were identified in the enhancer
and in the 3' end of intron 1. Expression of hCD4 in macrophages is
controlled by at least 2 sets of regulatory elements: one present in
front of exon 1 and the second at the 5' end of intron 1. The hCD4
elements required for expression on both myeloid and lymphoid
CD8 + dendritic cells (DCs) from lymph node and thymus
were found to be different from those required for macrophage
expression. The results indicate that expression of hCD4 in T cells,
macrophages, and DCs is controlled by distinct regulatory elements.
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